Annexin A2 Egress during Calcium-Regulated Exocytosis in Neuroendocrine Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies and Reagents
2.2. Chromaffin Cell Culture and Transfection
2.3. Cell Stimulation and Extracellular AnxA2 and t-PA Measurements
2.4. Immunofluorescence and Confocal Microscopy
2.5. Plasma Membrane Sheet Preparation and Transmission Electron Microscopy Observation
2.6. Statistical Analysis
3. Results
3.1. AnxA2 Crosses the Plasma Membrane in Stimulated Chromaffin Cells in a Ca2+-Dependent Manner and Accumulates on the Extracellular Membrane Leaflet
3.2. Tyr 23 Phosphorylated AnxA2 Tetramer Binds the External Face of the Plasma Membrane
3.3. The Egress of AnxA2 is Linked to Exocytosis
3.4. Cell Surface Membrane-Associated t-PA is Present Close to Exocytotic Sites
3.5. AnxA2 and t-PA are Side by Side at Cell Surface of Stimulated Chromaffin Cells
4. Discussion
4.1. By Which Mechanism is AnxA2 Translocated to the Cell Surface in Chromaffin Cells?
4.2. What Might be the Role of Cell Surface AnxA2?
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gabel, M.; Royer, C.; Thahouly, T.; Calco, V.; Gasman, S.; Bader, M.-F.; Vitale, N.; Chasserot-Golaz, S. Annexin A2 Egress during Calcium-Regulated Exocytosis in Neuroendocrine Cells. Cells 2020, 9, 2059. https://doi.org/10.3390/cells9092059
Gabel M, Royer C, Thahouly T, Calco V, Gasman S, Bader M-F, Vitale N, Chasserot-Golaz S. Annexin A2 Egress during Calcium-Regulated Exocytosis in Neuroendocrine Cells. Cells. 2020; 9(9):2059. https://doi.org/10.3390/cells9092059
Chicago/Turabian StyleGabel, Marion, Cathy Royer, Tamou Thahouly, Valérie Calco, Stéphane Gasman, Marie-France Bader, Nicolas Vitale, and Sylvette Chasserot-Golaz. 2020. "Annexin A2 Egress during Calcium-Regulated Exocytosis in Neuroendocrine Cells" Cells 9, no. 9: 2059. https://doi.org/10.3390/cells9092059