Effect of Circadian Clock and Light–Dark Cycles in Onchidium reevesii: Possible Implications for Long-Term Memory
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Training Experiments
2.3. Cloning of cry1 and per2 Genes and Quantitative Analysis of OnCREB1, cry1, and per2 Genes
3. Results
3.1. Learning/Memory Acquisition and Long-Term Memory Expressed a Certain Rhythm in Different Conditions
3.2. Expressions of cry1, per2, and OnCREB1 under The Control of Light–Dark (LD) and Constant Darkness (DD) Cycles
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Usage | Primer Name | Primer Sequence (5′-3′) | Description |
---|---|---|---|
RT-PCR | Test-1F | GTTTAAAACGCCACCCCCAC | Used to amplify one part of the per2 fragment |
Test-1R | ATGCTGAACTGAGTGGGTGG | ||
Test-2F | TTCGAAAACTGGGTGCAGGT | Used to amplify one part of the per2 fragment | |
Test-2R | GTCTCAGGCTCTCTCATGCC | ||
Test-3F | ATGAGAGAGCCTGAGACCGT | Used to amplify one part of the per2 fragment | |
Test-3R | TGCGCATGTTCAGAAGGAGT | ||
Test-4F | TCGTGAATCAGCGGACAACA | Used to amplify one part of the per2 fragment | |
Test-4R | GAGGGCGATAGAAGCCAGTC | ||
Test-5F | ACATCCCACCAGAAACGTCC | Used to amplify one part of the per2 fragment | |
Test-5R | ATGGGCTCCTGAAACATGGG | ||
Test-6F | CAGGTGATGACTGGCTTCTATC | Used to amplify one part of the per2 fragment | |
Test-6R | CTGGTGTGTCGTAGTTCTCATC | ||
Test-7F | CCCTAAGGATGGGTCCCTCA | Used to amplify one part of the per2 fragment | |
Test-7R | CACCGTGGCTGTTGTTGATG | ||
Test-8F | AAACCCTCACTGCCCAGATG | Used to amplify one part of the cry1 fragment | |
Test-8R | GTGAGAAAGCAAGCCACAGC | ||
Test-9F | GTGTACAAGAGGCTGGTGCT | Used to amplify one part of the cry1 fragment | |
Test-9R | AAAAGCCCTCAGGAGCTGAC | ||
Test-10F | GTCAGCTCCTGAGGGCTTTT | Used to amplify one part of the cry1 fragment | |
Test-10R | CCTTGTCTGCGTCACAAAGC | ||
Test-11F | GGTGCCTCACAAGTAGGAATTA | Used to amplify one part of the cry1 fragment | |
Test-11R | GATTGTGTTCCCACGGTATCT | ||
Test-12F | GATGCTGACTGGAGCGTAAA | Used to amplify one part of the cry1 fragment | |
Test-12R | CCCTCAGGAGCTGACTAATAAAC | ||
Test-13F | AGACTGCAGACCCTGTTAATG | Used to amplify one part of the cry1 fragment | |
Test-13R | ATGGGCTAGAGAGCTGATACT | ||
Test-14F | ATGGAAACAGCCCACCACTC | Used to amplify one part of the cry1 fragment | |
Test-14R | TCCACCACGAAACTGAGCTG | ||
Test-15F | AGCTCAGTTTCGTGGTGGAG | Used to amplify one part of the cry1 fragment | |
Test-15R | GGGGATAGTCCTTGCCGATG | ||
RACE | 3′RACE-F1 | CCCTAAGGATGGGTCCCTCA | Gene-specific outer primer for per2 |
3′RACE-F2 | AATGTGTCTCTCAGCCCTGC | Gene-specific inner primer for per2 | |
3′RACE-F3 | GTCAGCTCCTGAGGGCTTTT | Gene-specific outer primer for cry1 | |
3′RACE-F4 | AGACTGCAGACCCTGTTAATG | Gene-specific inner primer for cry1 | |
3′RACE outer primer | TACCGTCGTTCCACTAGTGATTT | Primers from kit | |
3′RACE inner primer | CGCGGATCCTCCACTAGTGATTTCACTATAGG | ||
5′RACE-R1 | ATGCTGAACTGAGTGGGTGG | Gene-specific outer primer for per2 | |
5′RACE-R2 | AGACGAATGCTGCTGCTCTT | Gene-specific inner primer for per2 | |
5′RACE-R3 | GATTGTGTTCCCACGGTATCT | Gene-specific outer primer for cry1 | |
5′RACE-R4 | TCCACCACGAAACTGAGCTG | Gene-specific inner primer for cry1 | |
5′RACE outer primer | CATGGCTACATGCTGACAGCCTA | Primers from kit | |
5′RACE inner primer | CGCGGATCCACAGCCTACTGATGATCAGTCGATG | ||
qRT-PCR | qRT-PCR primer F | TGTTGAGTCCGCCAACCTTT | Used to amplify the per2 fragment for real-time PCR |
qRT-PCR primer R | AGTGGCTGCTCCTCTGAAAC | ||
qRT-PCR primer F | GATGCTGACTGGAGCGTAAA | Used to amplify the Cry1 fragment for real-time PCR | |
qRT-PCR primer R | CCAAAGCCCACAGGACAATA | ||
qRT-PCR primer F | CCAGTTGGAGGAACCAATGT | Used to amplify the OnCREB1 fragment for real-time PCR | |
qRT-PCR primer R | CATGTGCTGTGGACTTGAAATAG | ||
18S primer F | TCCGCAGGAGTTGCTTCGAT | Used to amplify the 18S fragment for real-time PCR | |
18S primer R | ATTAAGCCGCAGGCTCCACT |
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Xu, G.; Yang, T.; Shen, H. Effect of Circadian Clock and Light–Dark Cycles in Onchidium reevesii: Possible Implications for Long-Term Memory. Genes 2019, 10, 488. https://doi.org/10.3390/genes10070488
Xu G, Yang T, Shen H. Effect of Circadian Clock and Light–Dark Cycles in Onchidium reevesii: Possible Implications for Long-Term Memory. Genes. 2019; 10(7):488. https://doi.org/10.3390/genes10070488
Chicago/Turabian StyleXu, Guolyu, Tiezhu Yang, and Heding Shen. 2019. "Effect of Circadian Clock and Light–Dark Cycles in Onchidium reevesii: Possible Implications for Long-Term Memory" Genes 10, no. 7: 488. https://doi.org/10.3390/genes10070488