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Genes 2017, 8(1), 27; doi:10.3390/genes8010027

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
Gene-marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 18 October 2016 / Revised: 24 December 2016 / Accepted: 5 January 2017 / Published: 11 January 2017
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [1047 KB, uploaded 11 January 2017]   |  


Keratin-associated proteins (KAPs) are structural components of wool and hair fibers. To date, eight high glycine/tyrosine KAP (HGT-KAP) families have been identified in humans, but only three have been identified in sheep. In this study, the putative ovine homolog of the human KAP22-1 gene (KRTAP22-1) was amplified using primers designed based on a human KRTAP22-1 sequence. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) was used to screen for variation in KRTAP22-1 in 390 Merino × Southdown-cross lambs and 75 New Zealand (NZ) Romney sheep. Three PCR-SSCP banding patterns were detected and DNA sequencing revealed that the banding patterns represented three different nucleotide sequences (A–C). Two single nucleotide polymorphisms (SNPs) were identified in these sequences. Variant B was most common with a frequency of 81.3% in NZ Romney sheep, while in the Merino × Southdown-cross lambs, A was more common with a frequency of 51.8%. The presence of B was found to be associated with increased wool yield and decreased mean fiber curvature (MFC). Sheep of genotype BB or AB had a higher wool yield than those of genotype AA. These results suggest that ovine KRTAP22-1 variation may be useful when developing breeding programs based on increasing wool yield, or decreasing wool curvature. View Full-Text
Keywords: Keratin-associated protein KAP22-1; variation; mean fiber curvature (MFC); wool yield; sheep Keratin-associated protein KAP22-1; variation; mean fiber curvature (MFC); wool yield; sheep

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, S.; Zhou, H.; Gong, H.; Zhao, F.; Wang, J.; Liu, X.; Luo, Y.; Hickford, J.G.H. Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits. Genes 2017, 8, 27.

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