Next Article in Journal
Adaptation of an Existing Intake Structure Caused by Increased Sediment Level
Previous Article in Journal
The Application of a Modified Version of the SWAT Model at the Daily Temporal Scale and the Hydrological Response unit Spatial Scale: A Case Study Covering an Irrigation District in the Hei River Basin
Previous Article in Special Issue
Soil Media CO2 and N2O Fluxes Dynamics from Sand-Based Roadside Bioretention Systems
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Water 2018, 10(8), 1065; https://doi.org/10.3390/w10081065

Dynamics and Functional Potential of Stormwater Microorganisms Colonizing Sand Filters

Environmental Health and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
*
Author to whom correspondence should be addressed.
Received: 29 June 2018 / Revised: 24 July 2018 / Accepted: 9 August 2018 / Published: 10 August 2018
(This article belongs to the Special Issue Plant and Microbial Processes in Stormwater Treatment Systems)
View Full-Text   |   Download PDF [1549 KB, uploaded 10 August 2018]   |  

Abstract

Stormwater management is increasingly relying on engineered infiltration systems (EIS) to reduce the volume and improve the quality of managed stormwater. Yet, EIS in the field will be colonized by a diverse array of environmental microorganisms that change the physiochemical properties of the EIS and provide a habitat for microorganisms with harmful or beneficial qualities. Understanding factors influencing the composition and stability of microbial communities could open up strategies for more efficient management of stormwater. Here, we analyzed the potential pathogenic and metabolic capabilities of stormwater microorganisms colonizing idealized EIS (i.e., sand columns) under laboratory conditions over time. The diversity of microbial communities was analyzed using 16S rRNA gene sequencing, and potential pathogens and denitrifying microbes were identified from taxonomic match to known species. Denitrification potential as determined by nosZ abundance was also assessed with quantitative polymerase chain reaction PCR. Our findings demonstrate that replicate microbial communities colonizing sand columns change in a similar way over time, distinct from control columns and the source community. Potential pathogens were initially more abundant on the columns than in the stormwater but returned to background levels by 24 days after inoculation. The conditions within sand columns select for potential denitrifying microorganisms, some of which were also potential pathogens. These results demonstrate that a diverse suite of stormwater microorganisms colonize sand filters, including a transient population of potential pathogens and denitrifiers. Manipulating the inoculating microbial community of EIS could prove an effective mechanism for changing both potential pathogens and denitrifying bacteria. View Full-Text
Keywords: 16S rRNA gene; microbial community; diversity; potential pathogen; denitrification 16S rRNA gene; microbial community; diversity; potential pathogen; denitrification
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Fraser, A.N.; Zhang, Y.; Sakowski, E.G.; Preheim, S.P. Dynamics and Functional Potential of Stormwater Microorganisms Colonizing Sand Filters. Water 2018, 10, 1065.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Water EISSN 2073-4441 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top