UV-LEDs Efficiently Inactivate DNA and RNA Coliphages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Purification of Coliphages
2.2. Identification of Genetic Material of Coliphages with RNase Spot Test
2.3. UV Experiments
2.4. Calculations and Statistical Analyses
3. Results
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Coliphage Strains According to [22] | Host ATCC Strain | Genetic Material | Resistant (R), Intermediate Resistant (I) or Sensitive (S) | |
---|---|---|---|---|
Hg-UV [25] | Cl [32] | |||
MS2 | 15597 | RNA | R | I |
1 | 15597 | RNA | R | R |
5 | 15597 | RNA | R | R |
7 | 13706 | DNA | S | R |
17 | 13706 | DNA | R | R |
Coliphage Strains | Linear Regression Equations of UV-LEDs | Linear Regression Equations of Hg-UV | |
---|---|---|---|
y = Log10-Reduction x = Time (0–15 min) | y = Log10-Reduction x = Time (0–10 min) | y = Log10-Reduction x = Time (0–10 min) | |
MS2 | y = −(0.09 ± 0.030)x (R2 = 0.82) | y = −(0.11 ± 0.047)x (R2 = 0.91) | y = −(0.32 ± 0.003)x (R2 = 0.95) |
1 | y = −(0.25 ± 0.057)x (R2 = 0.68) | y = −(0.53 ± 0.081)x (R2 = 0.60) | y = −(0.69 ± 0.016)x (R2 = 0.96) |
5 | y = −(0.42 ± 0.057)x (R2 = 0.93) | y = −(0.54 ± 0,094)x (R2 = 0.99) | y = −(0.51 ± 0.012)x (R2 = 0.97) |
7 | y = −(0.33 ± 0.046)x (R2 = 0.79) | y = −(0.59 ± 0.044)x (R2 = 0.71) | y = −(0.93 ± 0.013)x (R2 = 0.85) |
17 | y = −(0.30 ± 0.049)x (R2 = 0.82) | y = −(0.49 ± 0.079)x (R2 = 0.94) | y = −(0.56 ± 0.019)x (R2 = 0.91) |
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Zyara, A.M.; Heinonen-Tanski, H.; Veijalainen, A.-M.; Torvinen, E. UV-LEDs Efficiently Inactivate DNA and RNA Coliphages. Water 2017, 9, 46. https://doi.org/10.3390/w9010046
Zyara AM, Heinonen-Tanski H, Veijalainen A-M, Torvinen E. UV-LEDs Efficiently Inactivate DNA and RNA Coliphages. Water. 2017; 9(1):46. https://doi.org/10.3390/w9010046
Chicago/Turabian StyleZyara, Alyaa M., Helvi Heinonen-Tanski, Anna-Maria Veijalainen, and Eila Torvinen. 2017. "UV-LEDs Efficiently Inactivate DNA and RNA Coliphages" Water 9, no. 1: 46. https://doi.org/10.3390/w9010046
APA StyleZyara, A. M., Heinonen-Tanski, H., Veijalainen, A. -M., & Torvinen, E. (2017). UV-LEDs Efficiently Inactivate DNA and RNA Coliphages. Water, 9(1), 46. https://doi.org/10.3390/w9010046