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Insects 2012, 3(3), 763-788; doi:10.3390/insects3030763

Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management

Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Woodbridge Road, Menangle, NSW 2568, Australia
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Received: 13 June 2012 / Revised: 31 July 2012 / Accepted: 1 August 2012 / Published: 14 August 2012
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Abstract

Parasitoid detection and identification is a necessary step in the development and implementation of fruit fly biological control strategies employing parasitoid augmentive release. In recent years, DNA-based methods have been used to identify natural enemies of pest species where morphological differentiation is problematic. Molecular techniques also offer a considerable advantage over traditional morphological methods of fruit fly and parasitoid discrimination as well as within-host parasitoid identification, which currently relies on dissection of immature parasitoids from the host, or lengthy and labour-intensive rearing methods. Here we review recent research focusing on the use of molecular strategies for fruit fly and parasitoid detection and differentiation and discuss the implications of these studies on fruit fly management. View Full-Text
Keywords: Diptera; Tephritidae; parasitoid; biological control; DNA barcode; PCR; microsatellites Diptera; Tephritidae; parasitoid; biological control; DNA barcode; PCR; microsatellites
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Jenkins, C.; Chapman, T.A.; Micallef, J.L.; Reynolds, O.L. Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management. Insects 2012, 3, 763-788.

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