Identification and Active Evaluation of Antioxidant Peptides from Protein Hydrolysates of Skipjack Tuna (Katsuwonus pelamis) Head
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of the Protein Hydrolysate (KPH) of Kipjack Tuna Heads
2.3. Preparation of APs from KPH
2.3.1. Ultrafiltration
2.3.2. Separation of APs by Chromatography Methods
2.4. Analysis of Amino Acid Sequence and MW
2.5. Antioxidant Activity
2.6. Stability Properties
2.7. Statistical Analysis
3. Results and Discussion
3.1. Preparation of the Protein Hydrolysate (KPH) of Kipjack Tuna Heads
3.2. Purification of APs from KPH
3.2.1. Ultrafiltration
3.2.2. Anion-Exchange Chromatography
3.2.3. Gel Filtration Chromatography (GFC)
3.2.4. Purification of APs from GC-3 by Reversed-Phase HPLC (RP-HPLC)
3.3. Molecular Structural Analysis
3.4. Antioxidant Activity
3.4.1. Radical Scavenging Activity
DPPH Radical Scavenging Activity
Hydroxyl Radical Scavenging Activity
Superoxide Anion Radical Scavenging Assay
3.4.2. Reducing power
3.4.3. Lipid Peroxidation Inhibition Assay
3.5. Effects of Thermal and pH Treatments on the Stability of P2, P4 and P6
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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No. | RT (min) | Theoretical Mass/Observed Mass (Da) | Amino Acid Sequence |
---|---|---|---|
P1 | 9.05 | 375.37/375.39 | VEE |
P2 | 11.36 | 783.89/783.90 | WMFDW |
P3 | 12.63 | 788.84/788.85 | DAGPYGPI |
P4 | 13.25 | 652.76/652.79 | WMGPY |
P5 | 16.78 | 861.94/861.95 | ERGPLGPH |
P6 | 17.22 | 503.57/503.58 | EMGPA |
No. | EC50 (mg/mL) | ||
---|---|---|---|
DPPH Radical | Hydroxyl Radical | Superoxide Anion Radical | |
P1 | 3.76 ± 0.16 a | 2.43 ± 0.11 a | 1.79 ± 0.11 a |
P2 | 0.31 ± 0.02 b | 0.30 ± 0.03 b | 0.56 ± 0.04 b |
P3 | 1.33 ± 0.09 c | 1.71 ± 0.06 c | 1.51 ± 0.11 c |
P4 | 0.33 ± 0.02 b | 0.43 ± 0.02 d | 0.38 ± 0.03 d |
P5 | 0.93 ± 0.04 d | 0.81 ± 0.04 e | 3.04 ± 0.18 e |
P6 | 0.46 ± 0.04 e | 0.52 ± 0.04 d | 0.71 ± 0.04 b |
GSH | 0.22 ± 0.01 b | 0.12 ± 0.01 f | 0.09 ± 0.01 f |
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Zhang, L.; Zhao, G.-X.; Zhao, Y.-Q.; Qiu, Y.-T.; Chi, C.-F.; Wang, B. Identification and Active Evaluation of Antioxidant Peptides from Protein Hydrolysates of Skipjack Tuna (Katsuwonus pelamis) Head. Antioxidants 2019, 8, 318. https://doi.org/10.3390/antiox8080318
Zhang L, Zhao G-X, Zhao Y-Q, Qiu Y-T, Chi C-F, Wang B. Identification and Active Evaluation of Antioxidant Peptides from Protein Hydrolysates of Skipjack Tuna (Katsuwonus pelamis) Head. Antioxidants. 2019; 8(8):318. https://doi.org/10.3390/antiox8080318
Chicago/Turabian StyleZhang, Lun, Guo-Xu Zhao, Yu-Qin Zhao, Yi-Ting Qiu, Chang-Feng Chi, and Bin Wang. 2019. "Identification and Active Evaluation of Antioxidant Peptides from Protein Hydrolysates of Skipjack Tuna (Katsuwonus pelamis) Head" Antioxidants 8, no. 8: 318. https://doi.org/10.3390/antiox8080318