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Review

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

by
Ana Villares
*,
Laura Mateo-Vivaracho
and
Eva Guillamón
Centre for the Food Quality, National Institute for Agriculture and Food Research and Technology (INIA), Campus Universitario Duques de Soria, c/José Tudela s/n, 42004, Soria, Spain
*
Author to whom correspondence should be addressed.
Agriculture 2012, 2(4), 452-471; https://doi.org/10.3390/agriculture2040452
Submission received: 19 October 2012 / Revised: 12 November 2012 / Accepted: 10 December 2012 / Published: 18 December 2012
(This article belongs to the Special Issue Healthy Benefits of Nutraceutical, Phytochemicals in Agricultural Products)
Browse Figure
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Abstract

:
Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

Graphical Abstract

1. Introduction

Edible mushrooms have been traditionally appreciated due to their excellent sensory characteristics, including their unique aroma and taste. Mushrooms contain a wide range of molecules responsible for the fungal flavor comprising of volatile compounds [1] and unsaturated fatty acids, as well as amino acids [2,3]. Besides their excellent flavor, mushrooms have attracted much attention due to their proven healthy properties. Mushrooms contain low fat levels and low energy values, though they contain high amounts of proteins and essential amino acids. Furthermore, edible mushrooms can be considered as a source of dietary fiber, since the contents usually range from 4% to 55% (dried matter) [4,5,6].
Both cultivated and wild edible mushrooms have been a matter of research as therapeutic agents, and several bioactive substances have been identified showing health-promoting benefits [7]. Different functional compounds have been found in mushrooms, including phenolic compounds, sterols, terpenes, ceramides, etc. Among them, polysaccharides can be considered as one of the main groups of compounds occurring in mushrooms that are responsible for a great range of healthy properties. Recently, several polysaccharides from mushrooms have been isolated and characterized, such as lentinan from Lentinus edodes, pleuran from Pleurotus mushrooms, schizophyllan from Schizophyllum commune, or ganoderan and ganopoly from Ganoderma lucidum. Generally, bioactive polysaccharides from mushrooms consist of a β-linked glucose backbone displaying different patterns and degrees of branching. However, other polysaccharides formed by galactose or mannose have been also found in mushrooms, as well as heteropolysaccharides and glucan-protein complexes.
Polysaccharides from mushrooms have shown a great range of healthy properties, being the most important modulation of the immune system. Bioactive carbohydrates, including β-glucans, are usually viewed as biological response modifiers (BRM) [8]. Immunological modulation is involved in the prevention of several diseases, including cancer or pathogen infections, since the polysaccharide may enhance the immune system. The molecular mechanism of biological activity of polysaccharides may involve several pathways. Physical-chemical properties, such as molecular weight, primary structure, solution conformation, and polymer charge, among others, may play a role in determining whether and with what affinity polysaccharides bind to the receptors and show the biological activity.
This article reviews the main methods for polysaccharide extraction, isolation and purification, as well as the most important analytical techniques used for the structural characterization of the carbohydrates. In addition, some of the most important polysaccharides from mushrooms are described along with the healthy benefits they provide.

2. Extraction of Polysaccharides

Higher Basidiomycete mushrooms contain biologically active polysaccharides in the fruiting bodies, the cultured mycelium, and the cultured broth. The extraction yield of polysaccharides from fungal material depends not only on the sample nature, but also on different parameters, for instance, temperature, pressure, pH and ionic strength of the solvent, and fineness of solid particle grinding. Polysaccharides extraction is commonly performed in aqueous solvents, generally water or basic aqueous solutions. Mushroom samples are first freeze-dried, and then ground to a fine powder.

2.1. Conventional Extraction

Conventional methods usually involve stirring the sample into aqueous solutions. Generally, samples are subjected to successive extractions [9]. Firstly, polysaccharide extraction is carried out with water at room temperature. After centrifugation, a clear supernatant is separated from the residue. The residue is secondly extracted with boiling water and again separated from the liquid by centrifugation. The remainder solid may be subsequently extracted with aqueous basic solutions (NaOH or KOH, 2% w/v) at 100 °C. Again, the residue is separated by centrifugation and the solid is discarded.
Water or basic aqueous solutions may not be strong enough for separating water-insoluble polysaccharides. For this purpose, acidic solutions may be employed. Glucans extracted with acid are typically non-branched or branched at a very low frequency. Several acids, including acetic, formic, hydrochloric, and phosphoric, have been tested for the isolation of water insoluble β-(1→3)-glucans from Saccharomyces cerevisiae [10]. Results showed that the primary structure, in terms of monosaccharide composition and side-chain branching, was not substantially altered, regardless of the acid employed. On the contrary, molecular mass, size, polydispersity and intrinsic viscosity of the glucan obtained were influenced by the pKa of the protic acid employed. The results suggested that the weaker the protic acid used, the greater the molecular mass and polydispersity of the glucan.

2.2. Pressurized Water Extraction

Sophisticated techniques, although not used extensively, may be a good strategy for isolating polysaccharides. Traditional solvent extraction techniques use large quantities of toxic organic solvents, are generally very laborious, and possess low selectivity and/or low extraction yields. Furthermore, sometimes conventional extraction can expose the extracts to excessive heat, light, and oxygen, and requires long extraction times, which may favor the activity of glucan-degrading enzymes.
Pressurized liquid extraction (PLE) is an emerging technique that presents important advantages over traditional extractions. During the extraction process, the solvents inside the PLE extraction cartridge are at high temperature and high pressure, near their supercritical region. The high temperature makes possible the high solubility and high diffusion rate while the high pressure keeps the solvent below its boiling point. PLE uses less solvent quantities in a shorter period of time since at high pressures and high temperatures the solvents penetrate the solid samples at a much higher rate permitting a fast and efficient extraction process with minimal solvent usage. Furthermore, PLE is automated, and involves retaining the sample in an oxygen and light-free environment. In this field, water has been employed to isolate polysaccharides from Lentinus edodes. Several pressures and times of extraction were tested in order to optimize the extraction conditions. When time was increased from 10 to 70 min, the polysaccharide recovery increased from 80.6% to 88.6%. Further increases in time did not improve the yield. In regards to pressure of extraction, the greater the pressure, the higher the yield until 15.2 MPa, where it no longer produces an increase in polysaccharide recovery. Therefore, a pressure of 10.1 MPa for 70 min at 28 °C seem to be the optimal conditions to increase polysaccharide recovery for the isolation of α-(1→4) and β-(1→6) glucans [11]. Depending on the polysaccharide structure and source, extraction operating conditions may vary. For instance, other authors have described the isolation of β-(1→3)-glucans from seaweeds. In this case, pressurized water extraction operated at 150 °C for 20 min and the recovery was 13.8% [12].

2.3. Supercritical Fluid Extraction

Supercritical fluid extraction (SFE) has been previously used for the extraction of bioactive compounds [13]. SFE consists of the extraction by using a solvent at a temperature and a pressure above its supercritical point. Near to the critical point, small changes in pressure or temperature result in large changes in density, which allows for the control of many properties of the supercritical fluid. Carbon dioxide is commonly used as a solvent since CO2 is environmentally friendly, has a relatively high liquid-like density, low viscosity and high diffusivity. These properties provide a very special solvent that is effective both at dissolving materials and penetrating solid matrices. During supercritical carbon dioxide (SC-CO2) extraction, the temperature is relatively low and organic solvents are excluded, so decomposition of the active components is avoided. In addition, during the dynamic process, fresh supercritical CO2 flows through materials continuously and the mass transfer is intensive, which may result in a satisfactory performance.
Process parameters, including operating pressure, temperature, time and CO2 flow rate, must be investigated for the optimization of the SC-CO2 conditions. This technique was used to isolate carbohydrates from Ganoderma lucidum. When the flow rate was between 4 and 10 kg/h, the polysaccharide recoveries increased with the CO2 flow rate; however, when it exceeded 10 kg/h, the yields did not increase much further. The polysaccharide recoveries increased when the operating pressure changed from 27 to 35 MPa. Differently, the effect of temperature was influenced by the operating pressure. Thus, at low pressures, the higher the temperature, the greater the polysaccharide recovery. In contrast, at high pressures, the effect of the temperature (25–35 °C) was not substantial. Regarding extraction time, the recovery increased with time (4 h). Therefore, the optimum conditions for the extraction of polysaccharides from Ganoderma lucidum were pressure 35 MPa, temperature 25 °C, time 4 h and CO2 flow rate 10 kg/h [14].

2.4. Ultrasound-Assisted Extraction

Ultrasonic-assisted extraction (UAE) is an expeditious, inexpensive and efficient alternative to traditional extraction techniques and, in some cases, even to supercritical fluid and microwave-assisted extraction. The sound waves propagate into the extraction solvent and result in alternating high-pressure (compression) and low-pressure (rarefaction) cycles. During the low-pressure cycle, high-intensity ultrasonic waves create vacuum bubbles in the solvent, and when the bubbles attain a volume at which they can no longer absorb energy, they collapse violently during the high-pressure cycle so as to disrupt molecular interactions and favor extraction. This technique has been widely used in the fractionation of plant materials [15]. Operating parameters (ultrasonic power, ratio of water to sample, extraction time and extraction temperature) must be optimized to increase polysaccharide recovery. For instance, for the separation of polysaccharides from black fungus, the operating extraction conditions were ultrasonic power 350 W, a ratio of water to sample 5, and 35 min of extraction time at 90 °C [16]. Polysaccharides from Agaricus bisporus mushrooms were extracted at the following conditions: ultrasonic power 230 W, at 70 °C for 62 min, and a ratio of water volume to raw material weight of 30 mL/g, achieving a yield of 6.02% [17].

2.5. Microwave-Assisted Extraction

Microwave-assisted extraction (MAE) is an alternative approach to the traditional extraction methods. This technique, widely used in the extraction of bioactive compounds, provides a rapid sample preparation with reduced amounts of solvent. Microwaves heat up the molecules by ionic conduction and dipole rotation. If the extraction medium offers a resistance to the ion migration, a friction is generated and the minute microscopic traces of moisture occurring in the sample are heated. This process generates tremendous pressure on the cell wall until rupture and release of active compounds from the sample. Microwaves have been employed for the extraction of polysaccharides from Agaricus blazei Murrill [18]. The optimum conditions for a 12.35% recovery were extraction time 29.37 min, microwave power 400 W, extraction temperature 74.64 °C and ratio of water to material 32.7:1. In the case of Cordyceps militaris, the optimal conditions to obtain the highest polysaccharide yield were a microwave power of 744.8 W for 4.2 min and a ratio of solution to solid of 31.1 mL/g [19]. Similarly, three polysaccharide fractions were separated from Lycoris aurea [20].
The microwaves technique can be hyphenated with ultrasound-assisted extraction in order to reduce extraction time, increase efficiency and save energy. The experimental results confirm that ultrasonic/microwave assisted extraction (UMAE) of polysaccharides had great potential and efficiency compared with traditional hot water extraction. This coupled method was employed for the separation of polysaccharides from Lycium barbarum at the following optimized operating conditions: microwave power of 500 W for 10 min and ultrasonication for 30 min at 50 °C and pH 9.0 [21]. In the case of polysaccharides from Ganoderma lucidum, the optimal extraction conditions were ultrasonic power of 50 W, microwave power of 284 W, extraction time of 701 s and water/solid ratio of 11.6:1, respectively [22]. Using this coupled method, the yield of polysaccharides could be significantly improved compared with that of classical hot water extraction, reaching nearly 100% yield, and increased by 27.7% as against ultrasound-assisted extraction. Polysaccharides were also separated from Inonotus obliquus by microwave and ultrasound-assisted extraction at 90 W microwave power with 40 kHz ultrasonic frequency, and solid/water ratio was 1:20 (w/v) for 19 min [23]. Under these conditions, the recovery and purity of polysaccharides were 3.25% and 73.16%, respectively, which are above those of traditional hot water extraction.

3. Purification

Polysaccharides extracted from the source material are generally dissolved in water or aqueous solutions. Nevertheless, other macromolecules, such as proteins, may be also present in the medium. Therefore, several purification steps must be carried out to remove other substances. The samples may be subjected to a previous step consisting of a methanolic extraction in order to remove phenolic compounds, monosaccharides, amino acids and other related molecules. The elimination of phenolic derivatives is successful [24], and increases the effectiveness of extraction [25]. Proteins can be removed by precipitation with trifluoroacetic acid (20%, w/v) or by treatment with the enzyme protease at 40 °C for 1 h (pH 7.5). Then, proteins are separated by centrifugation.
After protein removal, polysaccharides are precipitated from the supernatants by the addition of ethanol in 2:1 ratio (v/v). Concentrated sodium chloride solutions can be added to favor the precipitation, and the solid can be washed with organic solvents, such as acetone or ethanol.
Once polysaccharides have been separated from other compounds, pure carbohydrate fractions are obtained; however, each fraction may contain several polysaccharides showing different molecular sizes. Fractionation can be performed by precipitation with ammonium sulfate. This method has been employed for polysaccharides from cereals and the dominant mechanism of fractionation is the molecular size [26,27].
Size exclusion chromatography (SEC) allows the separation of polysaccharides according to their size, and the subsequent determination of the molecular weight. Columns composed of hydroxylated polymers with residual carboxyl functionality are usually employed for the separation of polysaccharides. Elution is performed with diluted NaCl, NaNO3 or NaH2PO4 solutions at low flow rates. Polysaccharide detection is achieved by refractive index detectors or by postcolumn derivatization with calcofluor [28,29].

4. Available Techniques for Structural Elucidation

Structural analysis of polysaccharides requires the determination of molecular mass, chain composition, configuration and conformational isomers, sequence of monosaccharide residues, presence and position of branches and functional groups, and presence of interglycosidic linkages. Nowadays, there is a wide range of characterization techniques available to obtain structural details of polysaccharides. Chemical methods involve the use of reactions to get more insight into the chemical structure and arrangement. In contrast, analytical techniques are gaining more importance since sample preparation is straightforward and they give precise information.

4.1. Chain Composition of Monosaccharides

Monosaccharide composition is usually studied by the acid hydrolysis of the polysaccharides and the subsequent analysis of the resultant monosaccharides. Hydrolysis is usually performed with trifluoroacetic acid (TFA) at temperatures up to 120 °C. Other acids, such as H2SO4 or HCl, could also be employed [30].
Monosaccharide profile can be determined by high performance liquid chromatography (HPLC) or, after derivatization, by gas chromatography. Gas chromatography of alditol acetates is widely used for the identification and quantification of sugars forming the chain. Monosaccharides are first reduced to their corresponding alditols with sodium borohydride and then they are acetylated. Separation is usually achieved in polar stationary phases [31].

4.2. Presence and Position of Branches

O-methylation of polysaccharides is an important sample preparation step in structural analysis of complex carbohydrates. Other derivatives, such as trimethylsilyl, acetyl, trifluoroacetyl, methaneboronate, acetal, or a combination of them, can also be employed; however, O-methylated carbohydrates have been widely studied since the reaction confers to the structural analysis several advantages in the determination of the monosaccharide composition, the sequence of monosaccharide residues, and the position of branches. The permethylated carbohydrates are acid hydrolized to partially methylated monosaccharides and reduced to their corresponding alditols. The newly formed hydroxyl groups are acetylated to partially methylated and acetylated alditols, which are identified and quantified by gas chromatography coupled to mass spectrometry (GC-MS) [32]. MS is an indispensable technique that requires sample amounts in the range of picograms or less and not a high purity of polysaccharides. Although structural analysis by MS can be performed on many underivatized olygosaccharides and glycoconjugates, O-methylated polysaccharides are still preferred in order to increase the stability of ions and sensitivity of MS detection. Gas chromatography coupled to mass spectrometry provides useful information regarding the position of glycosidic linkage, the occurrence of branches, and monosaccharide composition in complex carbohydrates. Nevertheless, the MS analysis itself does not provide complete information about anomeric configuration, ring form and conformational isomers [33].
Nuclear magnetic resonance (NMR) can theoretically perform almost full structural analysis of underivatized complex carbohydrates. Nevertheless, the technique has some limits in its applicability. The major weakness of NMR spectroscopy as a method for structure determination is its poor sensitivity. Great sample amounts are usually required, and sample material should be homogeneous to avoid ambiguous assignments. NMR may provide information about pattern of branching; however, the interpretation of NMR data is limited by the complexity of the spectra obtained.

4.3. Configuration of the Glycosidic Bond

Polysaccharides occur in nature both in α and β configurations; however, the physiological properties are substantially different depending on the linkage type. Therefore, the elucidation of bond configuration is an important matter of research in the structural study of polysaccharides. NMR is a powerful tool for obtaining information about the monosaccharide composition and anomeric configuration. Comparison of chemical shifts and coupling constants with spectral data found in the literature for related polysaccharides provides relevant information about the anomeric configuration. At the 1H-NMR spectrum, the anomeric protons resonate at the region 4.3–5.9 ppm. If the configuration is α, the chemical shift is larger than β [34]. Furthermore, the α anomeric configuration shows 3J1,2 coupling constants of less than 4 Hz whereas β configuration presents coupling constants greater than 7.5 Hz [35]. Anomeric carbon signals resonate between 90 and 112 ppm at 13C-NMR experiments. Similarly to 1H-NMR chemical shifts, α anomers appear at 2–7 ppm higher field relative to the β anomer.
Infrared spectroscopy (IR) also provides information of anomeric configuration. For instance, the IR band at 850 cm−1 suggests an α configuration of the glucosyl motif [36] whereas higher frequencies (890 cm−1) are due to a β anomeric configuration [37].
Anomeric configuration can also be elucidated by chemical procedures. In this field, calcofluor has been found to be a useful and rapid chemofluorescent stain for detection of β configuration in polysaccharides. Calcofluor white, namely cellufluor, binds nonspecifically to β-linked polysaccharides and the sample fluoresces an intense green whereas α configurations do not exhibit fluorescence [38].

4.4. Conformational Arrangement

Methods such as 13C-NMR spectroscopy [39], multi-angle laser light scattering [40] or x-ray crystallography [41] have been previously used for the study of the tertiary structure of polysaccharides; however, sometimes their use is limited by availability, cost, and efficacy for evaluating carbohydrate structure in solution.
2D NMR spectroscopy provides valuable structural information related to sugar attachment sites [42]. Several techniques are available, for instance, Correlated Spectroscopy (COSY), Rotating frame Overhauser Enhancement Spectroscopy (ROESY), Nuclear Overhauser Effect Spectroscopy (NOESY), Heteronuclear Multiple Quantum Coherence (HMQC), or Heteronuclear Multiple Bond Coherence (HMBC), among others. These techniques give more insight into the correlation between atoms, which could help to elucidate the connectivities and dispositions of sugars.
Depending on their branching pattern, β-linked polysaccharides would arrange according to three main conformations: single helix, triple helix or random coil. These conformations are known to be related in an interconvertable manner in aqueous solution under certain physical and chemical treatments [43]. Chemical dyes specifically interact with certain conformations; therefore, complexation of polysaccharides with these dyes is a good strategy to get information about the polysaccharide conformation. For instance, single helix conformations are commonly found in β-(1→3)-linked polysaccharides. The presence of a single helix conformation may be detected by reaction with aniline blue in solution [44]. Glucans with high degrees of β-(1→6)-glycosidic bonds usually arrange into a triple helix. Polysaccharides showing a triple helix conformation form a complex with Congo red in alkaline solutions, which is stabilized by strong hydrogen bonds and/or hydrophobic interactions between the polysaccharide and the dye molecule. Complexation is characterized by the red shift of the absorption maximum of the Congo red solutions (489 nm) to longer wavelengths up to 520 nm [45].

5. Structural Features of Polysaccharides Occurring in Mushrooms

Bioactive polysaccharides have been isolated from several plant materials including yeasts and bacteria; in this field, the fungal kingdom was demonstrated as being a good source of bioactive carbohydrates. Among medicinal mushrooms, a polysaccharide isolated from Geastrum saccatum, consisting of a β-linked glucopyranosyl residue attached to a protein, has shown both antioxidant and anti-inflammatory activities [46]. Other mushrooms, such as Flammulina velutipes, contain complex carbohydrates. This fungus showed a polysaccharide composed of a 6-O-substituted galactopyranosyl main chain, partially substituted at O-2 by 3-O-D-mannopyranosyl-L-fucopyranosyl, α-D-mannopyranosyl, and, in a minor proportion, α-L-fucopyranosyl groups [47]. The moiety (1→3),(1→6)-linked D-glucose has been found in several fungi. The polysaccharides have been fully characterized and some of them even have a descriptive name. For instance, the (1→3),(1→6)-glucan isolated from Schizophyllum commune is termed schizophyllan [48]. Other mushrooms, such as Sparassis crispa, Phellinus linteus [25], and some of the genus Termitomyces [49,50], have also shown to possess β-D-glucans with different linkage patterns, depending on the fungal species.
Edible mushrooms could represent an excellent source of bioactive carbohydrates since they can be easily included in the diet, thus avoiding the isolation and purification processes required for the inedible species. In this field, a β-(1→3)-linked D-glucan with single unit glucosyl branches attached at O-6 of every third backbone residue has been isolated from Hericium erinaceus [51]. Heteropolysaccharides have been also isolated from this mushroom; thus, an α-(1→6)-linked D-galactopyranosyl with rhamnose and glucose branches at O-2 has been described [52]. A linear water-insoluble β-(1→3)-D-glucan have been extracted from the fruit body of Ganoderma lucidum [53], as well as a water-soluble (1→6)-linked glucan with (1→4) branches at O-4 [54], or a heteropolysaccharide composed of 1,4-linked α-D-glucopyranosyl and 1,6-linked β-D-galactopyranosyl residues with branches at O-6 of glucose and O-2 of galactose residues [55]. More complex structures showing antioxidant properties, such as a protein-bound polysaccharide has been also isolated from the genus Ganoderma, specifically from Ganoderma atrum [56]. A water-soluble neutral polysaccharide was extracted from Auricularia auricula-judae consisting of a β-(1→3)-D-glucan with two β-(1→6)-D-glucosyl residues for every three main chain glucose residues, showing a comb-branched structure [57].
The genus Agaricus has been widely studied. Hence, several polysaccharides have been found in the cell walls of Agaricus bisporus. The fractions contained the mucilage fraction composed of glucose and galactose, a second fraction with a xylomannan associated with the glucan and several fractions of glucans showing different attachment sites [32]. The Agaricus bitorquis mushrooms contained a water-soluble β-(1→3)-linked glucan [58], and a glucan-protein complex was isolated from Agaricus blazei mushrooms by extraction with water at 100 °C for 5 h. The glucan moieties showed both α and β linkages, being the β the more abundant [59].
Similarly, Lentinus edodes has attracted much interest. Besides lentinan, which is a (1→3),(1→6)-linked D-polysaccharide [60], a heteropolysaccharide consisting of a fucomannogalactan with a main chain of (1→6)-linked α-D-galactopyranosyl units branched at O-2 has been extracted [61], as well as a glucogalactan bound by (1→3),(1→4) linkages [62].
The genus Pleurotus has been also investigated; for instance, pleuran, isolated from Pleurotus ostreatus consists of a backbone of (1→3)-linked D-glucan every fourth residue, being substituted at O-6 with single D-glucopyranosyl groups [63]. The occurrence of different structural patterns between polysaccharides from Pleurotus species has been previously observed, for instance, a linear α-(1→3)-linked D-glucan has been isolated from P. ostreatus and P. eryngii [64], and a β-(1→3),(1→6)-linked glucan has been extracted from P. ostreatus [65]. Furthermore, the mushroom Pleurotus florida has shown different types of glucans, which are a water-soluble αβ-polysaccharide consisting of a main chain of α-(1→3)-linked D-glucan partially substituted at O-3 and O-6 by β-D-glucose [66] and a water-insoluble (1→3),(1→6)-linked β-D-glucan [67]. Complex carbohydrates have been found in the genus Pleurotus, for instance, a 3-O-methylated α-galactan [68], and a partially 3-O-methylated α-(1→4)-linked D-galactan and D-mannan [69].
Polysaccharides from Calocybe indica have received attention due to their immunoenhancing properties. Homopolysaccharides composed of glucose have been isolated from this mushroom, for instance a water-soluble αβ-(1→4),(1→6)-glucan [70], and a water-insoluble β-(1→3),(1→4)-glucan (termed calocyban) [71]. Mixed polysaccharides were also found in this mushroom and the structural analysis revealed that they were composed of α-(1→3)-linked galactose, β-(1→4),(1→6)-linked glucose and fucose branches [72].
Among the wild mushrooms, some fungi, such as Boletus erythropus, have also shown water-soluble β-glucans [73]. The structural elucidation showed that the polysaccharides are composed of (1→3)-linked glucose units with branches at O-6.
Table 1 reviews the main important structural features of polysaccharides isolated from mushrooms.
Table
Table 1. Structural features of polysaccharides isolated from mushrooms.
Table 1. Structural features of polysaccharides isolated from mushrooms.
MushroomType of polysaccharideStructural featuresRef.
Agaricus bisporusHeteropolysaccharideMucilage composed of glucose and galactose[32]
Agaricus bisporusHeteropolysaccharideXylomannan[32]
Agaricus bitorquisHomopolysaccharideβ-(1→3)-linked glucan[58]
Agaricus blazeiGlucan-Protein complexαβ-glucan[59]
Auricularia auricula-judaeHomopolysaccharideβ-(1→3)- D-glucan with branches at (1→6)[57]
Boletus erythropusHomopolysaccharide(1→3)-linked glucose with branches at O-6[73]
Calocybe indicaHomopolysaccharideαβ-(1→4),(1→6)-glucan[70]
Calocybe indicaHomopolysaccharideβ-(1→3),(1→4)-glucan[71]
Calocybe indicaHeteropolysaccharideα-(1→3)-linked galactose, with β-(1→4),(1→6)-glucose and fucose branches[72]
Flammulina velutipesHeteropolysaccharide6- O-galactopyranoses substituted at O-2 by 3-O-D-mannopyranosyl-L-fucopyranosyl, α-D-mannopyranosyl, and α-L-fucopyranosyl[47]
Ganoderma atrumProtein-glucan complexβ-linked mannose, galactose and glucose[56]
Ganoderma lucidumHomopolysaccharideβ-(1→3)-linked D-glucan[53]
Ganoderma lucidumHomopolysaccharide(1→6)-glucan with (1→4) branches at O-4[54]
Ganoderma lucidumHeterolpolysaccharideα-(1→4)-D-glucopyranosyl and β-(1→6)-D-galactopyranosyl with branches at O-6 of glucose and O-2 of galactose [55]
Geastrum saccatumGlucan-Protein complexβ-linked glucan[46]
Hericium erinaceusHomopolysaccharideβ-(1→3)-D-glucan with branches at O-6[51]
Hericium erinaceusHeterolpolysaccharideα-(1→6)-D-galactopyranosyl with rhamnose and glucose branches at O-2[52]
Lentinus edodesHomopolysaccharide(1→3),(1→6)-D-polysaccharide[60]
Lentinus edodesHeteropolysaccharideFucomannogalactan of (1→6)-linked α-D-galactopyranoses branched at O-2[61]
Phellinus linteusHomopolysaccharideβ-(1→3)-linked D-glucan[25]
Pleurotus eryngiiHomopolysaccharideα-(1→3)-linked D-glucan[64]
Pleurotus floridaHomopolysaccharideα-(1→3)-D-glucan branched at O-3 and O-6 by β-D-glucose[66]
Pleurotus floridaHomopolysaccharideβ-(1→3),(1→6)-D-glucan[67]
Pleurotus ostreatusHomopolysaccharide(1→3),(1→6)-D-polysaccharide[63]
Pleurotus ostreatusHomopolysaccharideα-(1→3)-D-glucan[64]
Schizophyllum communeHomopolysaccharide(1→3),(1→6)-D-glucan[48]
Sparasis crispaHomopolysaccharideβ-(1→3)-D-glucan[25]
Termitomyces eurhizusHomopolysaccharideβ-(1→3)-D-glucan[49]
Termitomyces microcapusHomopolysaccharideβ-D-glucan[50]

6. Healthy Properties

Mushrooms have been traditionally employed as medicinal agents due to their proven healthy properties. Among the occurring bioactive compounds, polysaccharides are responsible for a wide range of biological activities, the modulation of the immune system being the most studied. Polysaccharides from mushrooms showing a β-linkage have demonstrated a boost in the human immune system and the modulation of the immunological response under certain circumstances, thus they are commonly termed biological response modifiers (BRM). As a result of the activation of the host’s immune system, these polysaccharides show significant antitumor, antiviral and antimicrobial activity, among other effects.

6.1. Tumor Therapy

Polysaccharides from fungi have been widely employed in tumor therapies due to their properties as immunological enhancers. Research studies have demonstrated that polysaccharides isolated from different mushroom genera are capable of providing anti-tumoral activity, for instance, Agaricus, Calocybe, Ganoderma, Grifola, Inonotus, Lentinus, Phellinus, Pholiota, Pleurotus, etc. [74,75,76,77,78,79,80,81] The mitogenic activity of fungal polysaccharides involves several mechanisms, such as the antiproliferation of cancer cells and induction of apoptosis, the regulation of the immune system and the antimetastatic effects. These include the activation of macrophages, T lymphocytes and natural killer cells (NK), which are able to secrete inflammatory mediators and cytokines such as the α tumor necrosis factor (TNF-α), γ interferon (IFN-γ), or 1β interleukin (IL-1β), among others. Polysaccharides can depress the E-selectin protein and gene expressions, which inhibit tumoral cell-to-cell adhesion [74]. Other mechanisms include antiproliferative effects, apoptosis induction, and differentiation of the tumoral cells [80].
Fungal polysaccharides have been widely employed as coadjuvants in tumoral therapies. In this field, lentinan, aβ-(1→3),(1→6)-linked polysaccharide from Lentinus edodes, has been used in a combined treatment of patients with advanced or recurrent gastric or colorectal cancer. In a study carried out with 89 patients of stomach cancer, lifespan increased in 80 days when 2 mg of intravenous lentinan per week were used [82,83]. Similarly, lifespan increased in 106 days in patients with breast cancer when similar doses of lentinan were employed [84].
In a study carried out with 30 patients of breast cancer, the combination of lentinan with chemoendocrine therapy was more effective than chemoendocrine therapy alone on the improvement of hormonal parameters such as serum levels of estradiol, follicle-stimulating hormone, luteinizing hormone and prolactin. Lentinan seemed to exert a dose-reductive effect on epirubicin (EPIR) therapy for breast cancer patients, and that combination therapy of lentinan and low-dose EPIR was more effective for the augmentation of the host defense activity than that of lentinan and high-dose EPIR [85].
A combination of lentinan and interleukin 2 (IL-2) has shown a synergistic effect in the reduction of lung metastasis colony numbers (85%). Furthermore, 3 of 13 mice given the pre-operative combination treatment achieved complete cure. The cellular mechanism of the antimetastatic activity involved a tumor-associated antigen-specific delayed-type hypersensitivity response. These data suggested that the life-prolonging effect of the combination of lentinan and IL-2 was mediated by antigen-specific T cells and that the combination of pre- and post-operative therapy with lentinan and IL-2 may be effective to prevent cancer recurrence and metastasis after surgical resection [86].

6.2. Cardiovascular Disease

Fungal polysaccharides may be beneficial for protection against cardiovascular diseases and its complications. Edible mushrooms are good candidates to be included in a healthy diet for the prevention of atherosclerosis, due to their high fiber and low fat content. Indeed, the mechanisms of action involve anticoagulation and antiaggregation activity in the blood platelets, resulting in low total triglyceride and lipid levels. Furthermore, the hypocholesterolemic effect can be associated to the antioxidant activity. Administration of black fungus polysaccharides had significantly enhanced myocardium and blood antioxidant enzyme activities and reduced lipid peroxidation levels in high fat mice [16]. Studies with humans revealed that diets rich in β-glucans decreased the total cholesterol and LDL levels in serum, as well as the visceral fat area and the waist circumference in hypercholesterolemic patients [87,88]. At the intestinal level, β-glucans reduced the absorption of cholesterol and long chain fatty acids and, furthermore, these polysaccharides downregulated the genes involved in lipogenesis and lipid transport [89].

6.3. Antiviral Activity (HIV)

Although the present results are promising, there is a dearth of studies involving HIV treatment with polysaccharides from mushrooms. A study was carried out with 88 HIV-positive patients with CD4 levels of 200–500 cells/mm. The patients were treated with a combination of lentinan from Lentinus edodes and didanosine (ddI). The combination caused significant increases in CD4 levels up to 38 weeks, whereas ddI alone was significant at the 5% level at 14 weeks [90].

6.4. Antimicrobial Activity

The ability of polysaccharides from mushrooms to modulate the immune response makes them potential candidates for combination therapy with Calmette-Guerin bacillus (BCG), or as adjunct for BCG vaccination, especially in high-risk individuals. The combined intranasal application of lentinan (at a dose of 1 mg/kg), followed by administration of BCG, produced high level of alveolar macrophage activation. Pre-treatment with lentinan enhanced the local immunohistological response to BCG in lung and reduced the generalized side effects [91].

6.5. Antioxidant Activity

Oxidative damage is related to the prevalence of a great number of common diseases. Therefore, substances capable of acting as antioxidant agents usually prevent the development of certain diseases. Polysaccharides from different mushrooms showed free radical scavenging activity [92], superoxide radical scavenging activity, reducing properties, lipid peroxidation inhibition, suppression of proliferation and oxidative stress, etc. [93] The activity not only depended on the fungal species but also on the chemical structure and arrangement of the active polysaccharide [75,94,95,96]. Similarly, mixed carbohydrates, such as polysaccharide–peptide complexes, have also shown a potent antioxidant activity [97].

6.6. Other Effects

Besides the biological effects described previously, polysaccharides from mushrooms have attracted much attention due to their behavior as dietary fiber. On the one hand, a diet rich in β-linked polysaccharides improved the gastrointestinal tract motility and, on the other hand, they seemed to improve insulin resistance in patients with type 2 diabetes [98]. Furthermore, the β-linked polysaccharides produced a satiating effect and the studies showed a reduced energy intake in volunteers [99].
Finally, polysaccharides have shown other healthy effects, for instance anti-aging effects [100], or hepatoprotective activity [101].

7. Conclusions

Mushrooms can be viewed as an important source of bioactive polysaccharides. The most common pattern consists of a β-linked glucose backbone displaying branches at certain sugar residues; nevertheless, other polysaccharides, such as galactans or mannans, or even polysaccharide-protein complexes can also be found in mushrooms. These carbohydrates have been considered as biological response modifiers due to their ability to enhance the immune system and, therefore, prevent and treat several common diseases and promote health. Cancer, cardiovascular diseases, and viral and bacterial infections are among the most studied diseases treated with polysaccharides from mushrooms and the results show that these bioactive carbohydrates may be successfully used in their treatments.

Acknowledgments

The authors are grateful to Ministerio de Ciencia e Innovación and INIA (projects RTA2009-00049 and AT07-003) for financial support of this work.

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Villares, A.; Mateo-Vivaracho, L.; Guillamón, E. Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms. Agriculture 2012, 2, 452-471. https://doi.org/10.3390/agriculture2040452

AMA Style

Villares A, Mateo-Vivaracho L, Guillamón E. Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms. Agriculture. 2012; 2(4):452-471. https://doi.org/10.3390/agriculture2040452

Chicago/Turabian Style

Villares, Ana, Laura Mateo-Vivaracho, and Eva Guillamón. 2012. "Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms" Agriculture 2, no. 4: 452-471. https://doi.org/10.3390/agriculture2040452

APA Style

Villares, A., Mateo-Vivaracho, L., & Guillamón, E. (2012). Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms. Agriculture, 2(4), 452-471. https://doi.org/10.3390/agriculture2040452

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