The Effect of Chronic Exposure of Graphene Nanoplates on the Viability and Motility of A549 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Nanomaterials
2.2. Cell Culture and Treatment
2.3. Cytotoxicity Assays
2.3.1. WST-1 Assay
2.3.2. alamarBlue™ Assay
2.4. Determination of Number Cell Nuclei by ImageXpress Micro XLS
2.5. Cell Cycle Distribution Analysis
2.6. Annexin V/Propidium Iodide Cell Death Assay
2.7. Chemotactic Migration Using the xCELLigence CIM-Plate-16
2.8. Spontaneous Motility of the A549 Cells
2.9. Analysis of Metabolism
2.10. Analysis of Cell Morphology by Transmission Electron Microscopy (TEM)
2.11. Statistical Analysis
3. Results
3.1. Short-Term Cytotoxicity of Graphene
3.2. Long-Term Effects of the GP1 Nanomaterial
3.2.1. In Vitro Cytotoxicity
3.2.2. Analysis of Metabolism
3.2.3. Viability Analysis
3.2.4. Cell Cycle Distribution Analysis
3.2.5. Analysis of Cell Morphology by Transmission Electron Microscopy (TEM)
Acute Graphene Exposure
Long-Term Graphene Exposure
3.2.6. Effect of GP1 on A549 Cell Migration
3.2.7. Effect of GP1 on Spontaneous A549 Cell Motility
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Suspension Diluted in | Particle Size (nm) (Z-Average) | PdI | Average ζ-Potential (mV) |
---|---|---|---|
Sterile water | 179 ± 103 | 0.188 | −42.5 ± 6.9 |
Full medium | 208 ± 103 | 0.203 | −8.52 ± 15.3 |
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Šestáková, B.; Schröterová, L.; Bezrouk, A.; Čížková, D.; Elkalaf, M.; Havelek, R.; Rudolf, E.; Králová, V. The Effect of Chronic Exposure of Graphene Nanoplates on the Viability and Motility of A549 Cells. Nanomaterials 2022, 12, 2074. https://doi.org/10.3390/nano12122074
Šestáková B, Schröterová L, Bezrouk A, Čížková D, Elkalaf M, Havelek R, Rudolf E, Králová V. The Effect of Chronic Exposure of Graphene Nanoplates on the Viability and Motility of A549 Cells. Nanomaterials. 2022; 12(12):2074. https://doi.org/10.3390/nano12122074
Chicago/Turabian StyleŠestáková, Blanka, Ladislava Schröterová, Aleš Bezrouk, Dana Čížková, Moustafa Elkalaf, Radim Havelek, Emil Rudolf, and Věra Králová. 2022. "The Effect of Chronic Exposure of Graphene Nanoplates on the Viability and Motility of A549 Cells" Nanomaterials 12, no. 12: 2074. https://doi.org/10.3390/nano12122074