Next Article in Journal
Computational Methods for Metabolomic Data Analysis of Ion Mobility Spectrometry Data—Reviewing the State of the Art
Previous Article in Journal
Differentiating Hepatocellular Carcinoma from Hepatitis C Using Metabolite Profiling
Article Menu

Export Article

Open AccessArticle
Metabolites 2012, 2(4), 717-732; doi:10.3390/metabo2040717

Influence of the RelA Activity on E. coli Metabolism by Metabolite Profiling of Glucose-Limited Chemostat Cultures

Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
Centre for Microbial Innovation, School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1142, New Zealand
Author to whom correspondence should be addressed.
Received: 3 August 2012 / Revised: 13 September 2012 / Accepted: 28 September 2012 / Published: 12 October 2012
View Full-Text   |   Download PDF [755 KB, uploaded 12 October 2012]   |  


Metabolite profiling of E. coli W3110 and the isogenic DrelA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography–mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were assessed in the levels of amino acids and fatty acids and their precursor metabolites, especially when growing at the lower dilution rates, demonstrating differences in their metabolic behavior. Despite the fatty acid biosynthesis being the most affected due to the lack of the RelA activity, other metabolic pathways involving succinate, lactate and threonine were also affected. Overall, metabolite profiles indicate that under nutrient-limiting conditions the RelA-dependent stringent response may be elicited and promotes key changes in the E. coli metabolism. View Full-Text
Keywords: metabolomics; metabolite profiles; stringent response; relaxed phenotype metabolomics; metabolite profiles; stringent response; relaxed phenotype

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Carneiro, S.; Villas-Bôas, S.G.; Ferreira, E.C.; Rocha, I. Influence of the RelA Activity on E. coli Metabolism by Metabolite Profiling of Glucose-Limited Chemostat Cultures. Metabolites 2012, 2, 717-732.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top