Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea
Abstract
:1. Introduction
2. Results
2.1. Energy Metabolite Analysis
2.2. Steroid Profiling
2.3. Saccharide (Polar Metabolite) Analysis by GC-MS
3. Discussion
3.1. Cassytha–Ipomoea Interaction
3.2. Cuscuta–Momordica Interaction
3.3. Cuscuta–Cassytha Interaction
3.4. Plant Interaction Comparison
4. Materials and Methods
4.1. Plant Culture and Sample Preparation
4.2. Extraction of Energy Metabolites, Polar Metabolites Analysis
4.3. Extraction of Steroids
4.4. UFLC Condition
4.5. GC/MS Condition
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
ADP | Adenosine diphosphate |
AMP | Adenosine monophosphate |
ATP | Adenosine triphosphate |
EI | Electron ionization |
FAME | Fatty acid methyl ester |
FI | Field ionization |
GC-MS | Gas chromatography-mass spectrometry |
HCl | Hydrochloric acid |
HR | Hypersensitive response |
ODS | Octadecyl-silica |
PAD | Photo diode array detector |
SAR | Systemic acquired resistance |
TCA | Tricarboxylic acid cycle |
TFA | Trifluoro acetic acid |
UFLC | Ultra-high performance liquid chromatography |
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Furuhashi, T.; Nakamura, T.; Iwase, K. Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea. Plants 2016, 5, 43. https://doi.org/10.3390/plants5040043
Furuhashi T, Nakamura T, Iwase K. Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea. Plants. 2016; 5(4):43. https://doi.org/10.3390/plants5040043
Chicago/Turabian StyleFuruhashi, Takeshi, Takemichi Nakamura, and Koji Iwase. 2016. "Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea" Plants 5, no. 4: 43. https://doi.org/10.3390/plants5040043