Therapeutic Applications of Native and Engineered Saccharomyces Yeasts

Round 1

Reviewer 1 Report

The benefits of Saccharomyces cerevisiae, both native and modified, var. boulardii and other S.cerevisiae in therapeutic uses are explained in the review. This is a nice review and an important subject. I just have a couple of comments.

1 Line 71

The description for innate health benefits is inaccurate in figure 1, for instance, it repeated between “toxin proteolysis” and “inactivation of toxin”, as well as “interference with toxin binding” and “interference with toxicity mechanism”. Please revise this section to reflect properly.

2 Line 87

I'm not sure what the “target disorder” stands for. Perhaps it would be better to say it another way.

3 Line 149

It is advised to combine sections 2.1 and 2.2 into one. The contents of "2.2 innate non-probiotic benefits" also discuss the innate probiotic benefits.

4 Line 317

It is suggested that "biosensing and expression systems" should be used instead of "biosensing and signaling systems."

5 Line 152,388, 394,399,401,430,439,476

It should say "polysaccharides" instead of "oligosaccharides."

The english of this manuscript should be improved before resubmission. 

Author Response

Dear Reviewer 1,

I very much appreciate your kind comment and detailed suggestion. All items were edited following your comments, and corresponding revised areas were highlighted in green. Please find the attached document (20240106-fermentation_2804591_responses-SK.pdf); pages 1-3 contain my responses to your suggestions and queries, and page 7 describes additional edits. Also, please find the revised manuscript (20240106-fermentation_2804591_revision-SK.docx, uploaded separately).

Sincerely,

Suryang Kwak

------------------------------------------------------------------------------------------
Suryang Kwak, Ph. D.
Assistant Professor (Kwak Lab: https://kwaklab.info)
Kookmin University
Department of Bio & Fermentation Convergence Technology
77 Jeongneung-Ro, Seongbuk-Gu, Seoul 02707, Republic of Korea
[p] +82-2-910-5735 [i] https://skwak.link [t] @yeastalmighty

Author Response File: Author Response.pdf

Reviewer 2 Report

Well-written manuscript, but could be improved for better impact to get accepted. Section 2 speaks about the health benefits of Sb and Sc. Although the section is divided into innate probiotic and non-probiotic benefits, it needs further details and modifications regarding the level/type of evidence. The evidence needs to be clarified whether they are based on in vitro or in vivo (animal or human) studies. A table with a list of studies describing the study design, methodology, and main outcomes will add additional value to the manuscript.

An additional section related to any safety aspects related to native and engineered Saccharomyces yeasts is needed.

Author Response

Dear Reviewer 2,

Thank you very much for taking the time to review this manuscript and for your kind comment. The areas edited following your suggestion were highlighted in turquoise to sort the items from other edits in the revised manuscript (uploaded separately). Please find the attached document; pages 4-6 contain my responses to your suggestions and queries, and page 7 describes additional edits.

Sincerely,

Suryang Kwak

------------------------------------------------------------------------------------------
Suryang Kwak, Ph. D.
Assistant Professor (Kwak Lab: https://kwaklab.info)
Kookmin University
Department of Bio & Fermentation Convergence Technology
77 Jeongneung-Ro, Seongbuk-Gu, Seoul 02707, Republic of Korea
[p] +82-2-910-5735 [i] https://skwak.link [t] @yeastalmighty

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

The author has responded to all the comments appropriately. No further comments.