Biomimetics, Volume 6, Issue 4 (December 2021) – 19 articles

Molecularly imprinted polymer-based surface plasmon resonance sensor prepared using silver nanoparticles was designed for the selective recognition of Penicillin G (PEN-G) antibiotic from both aqueous solution and milk sample. PEN-G imprinted sensors (NpMIPs) SPR sensor was fabricated using poly (2-hydroxyethyl methacrylate-N-methacroyl-(L)-cysteine methyl ester)-silver nanoparticles-N-methacryloyl-L-phenylalanine methyl ester polymer by embedding silver nanoparticles (AgNPs) into the polymeric film structure. In addition, a non-imprinted (NpNIPs) SPR sensor was prepared by utilizing the same polymerization recipe without addition of the PEN-G template molecule to evaluate the imprinting effect. FTIR-ATR spectrophotometer, ellipsometer, contact angle measurements were used for the characterization of NpMIPs SPR sensors. The linear concentration range of 0.01–10 ng/mL PEN-G was studied for kinetic analyses. The augmenting effect of AgNPs used to increase the surface plasmon resonance signal response was examined using polymer-based PEN-G imprinted (MIPs) sensor without the addition of AgNPs. The antibiotic amount present in milk chosen as a real sample was measured by spiking PEN-G into the milk. According to the Scatchard, Langmuir, Freundlich and Langmuir–Freundlich adsorption models, the interaction mechanism was estimated to be compatible with the Langmuir model. Full article

Desertification is a growing risk for humanity. Studies show that water access will be the leading cause of massive migration in the future. For this reason, significant research efforts are devoted to identifying new sources of water. Among this work, one of the more interesting strategies takes advantage of atmospheric non-liquid water using water harvesting. Various strategies exist to harvest water, but many suffer from low yield. In this work, we take inspiration from a Mexican plant (Echeveria pulvinate) to prepare a material suitable for future water harvesting applications. Observation of E. pulvinate reveals that parahydrophobic properties are favorable for water harvesting. To mimic these properties, we leveraged a combination of 3D printing and post-functionalization to control surface wettability and obtain parahydrophobic properties. The prepared surfaces were investigated using IR and SEM. The surface roughness and wettability were also investigated to completely describe the elaborated surfaces and strongly hydrophobic surfaces with parahydrophobic properties are reported. This new approach offers a powerful platform to develop parahydrophobic features with desired three-dimensional shape. Full article

Differences in the surface interactions of non-stoichiometric nanocrystalline B-type carbonate-substituted hydroxyapatite (n-cHAp) with the amino acids L-Lysine hydrochloride (L-LysHCl) and L-Arginine hydrochloride (L-ArgHCl) in acidic and alkaline media were determined using structural and spectroscopic analysis methods. The obtained data confirm that hydroxyapatite synthesized using our technique, which was used to develop the n-cHAp/L-LysHCl and n-cHAp/L-ArgHCl composites, is nanocrystalline. Studies of molecular composition of the samples by Fourier transform infrared spectroscopy under the change in the charge state of L-Lysine in environments with different alkalinity are consistent with the results of X-ray diffraction analysis, as evidenced by the redistribution of the modes’ intensities in the spectra that is correlated with the side chains, i.e., amide and carboxyl groups, of the amino acid. During the formation of a biomimetic composite containing L-Lysine hydrochloride and n-cHAp, the interaction occurred through bonding of the L-Lysine side chain and the hydroxyl groups of hydroxyapatite, which created an anionic form of L-Lysine at pH ≤ 5. In contrast, in biocomposites based on L-Arginine and n-cHAp, the interaction only slightly depends on pH value, and it proceeds by molecular orientation mechanisms. The X-ray diffraction and infrared spectroscopy results confirm that changes in the molecular composition of n-cHAp/L-ArgHCl biomimetic composites are caused by the electrostatic interaction between the L-ArgHCl molecule and the carbonate-substituted calcium hydroxyapatite. In this case, the bond formation was detected by Fourier transform infrared (FTIR) spectroscopy; the vibrational modes attributed to the main carbon chain and the guanidine group of L-Arginine are shifted during the interaction. The discovered interaction mechanisms between nanocrystalline carbonate-substituted hydroxyapatite that has physicochemical properties characteristic of the apatite in human dental enamel and specific amino acids are important for selecting the formation conditions of biomimetic composites and their integration with the natural dental tissue. Full article

Recently, biological nanostructures became an important source of inspiration for plasmonics, with many described implementations and proposed applications. Among them are brochosome-inspired plasmonic microstructures—roughly spherical core-shell particles with submicrometer diameters and with indented surfaces. Our intention was to start from the nanoplasmonic point of view and to systematically classify possible alternative forms of brochosome-inspired metal-containing particles producible by the state-of-the-art nanofabrication. A wealth of novel structures arises from this systematization of bioinspired metal-containing nanocomposites. Besides various surface nanoapertures, we consider structures closely related to them in electromagnetic sense like surface nano-protrusions, shell reliefs obtained by nano-sculpting, and various combinations of these. This approach helped us build a new design toolbox for brochosome-inspired structures. Additionally, we used the finite elements method to simulate the optical properties of simple brochosome-inspired structures. We encountered a plethora of advantageous optical traits, including enhanced absorption, antireflective properties, and metamaterial behavior (effective refractive index close to zero or negative). We conclude that the presented approach offers a wealth of traits useful for practical applications. The described research represents our attempt to outline a possible roadmap for further development of bioinspired nanoplasmonic particles and to offer a source of ideas and directions for future research. Full article

Bio-based materials have found their way to the design and fabrication in the architectural context in recent years. Fungi-based materials, especially mycelium-based composites, are a group of these materials of growing interest among scholars due to their light weight, compostable and regenerative features. However, after about a decade of introducing this material to the architectural community, the proper ways of design and fabrication with this material are still under investigation. In this paper, we tried to integrate the material properties of mycelium-based composites with computational design and digital fabrication methods to offer a promising method of construction. Regarding different characteristics of the material, we found additive manufacturing parallel to bio-welding is an appropriate fabrication method. To show the feasibility of the proposed method, we manufactured a small-scale prototype, a tilted arch, made of extruded biomass bound with bio-welding. The project is described in the paper. Full article

According to the National Energy Plan in Panama, the construction sector is one of the most prosperous and impactful sectors in the economy and it is expected to expand due to population growth by almost 300% by 2050. However, this sector must work on the transition towards sustainability and resilience in the face of climate change, since its growth implies a high consumption of resources and the contribution of greenhouse gases. The need to establish practices and strategies that embrace the dimension of sustainability and a circular economy is imminent. Currently, there is little guidance in the reference framework beyond certifications in planning, management and evaluation tools for its implementation. Different studies vary in the number of phases and considerations for projects. Therefore, the present work proposes the development of a unified road map, with defined phases, practices and indicators based on principles inspired by nature, such as biomimicry (Greek words: “bio” means life and “mimesis”, imitation), and focuses on a circular economy, validated by construction professionals, where strengths, opportunities, skills and threats are identified with a high level of acceptance. This contributes to strengthening the field of sustainable construction project management and a precedent for Panama. Full article

Multifunctional biomaterials can pave a way to novel types of micro- and nanoelectromechanical systems providing benefits in mimicking of biological functions in implantable, wearable structures. The production of biocomposites that hold both superior electrical and mechanical properties is still a challenging task. In this study, we aim to fabricate 3D printed hydrogel from a biocomposite of bovine serum albumin with graphene oxide (BSA@GO) using femtosecond laser processing. We have developed the method for functional BSA@GO composite nanostructuring based on both two-photon polymerization of nanofilaments and direct laser writing. The atomic-force microscopy was used to probe local electrical and mechanical properties of hydrogel BSA@GO nanowires. The improved local mechanical properties demonstrate synergistic effect in interaction of femtosecond laser pulses and novel composite structure. Full article

Biomimetics is an emerging field of science that adapts the working principles from nature to fine-tune the engineering design aspects to mimic biological structure and functions. The application mainly focuses on the development of medical implants for hard and soft tissue replacements. Additive manufacturing or 3D printing is an established processing norm with a superior resolution and control over process parameters than conventional methods and has allowed the incessant amalgamation of biomimetics into material manufacturing, thereby improving the adaptation of biomaterials and implants into the human body. The conventional manufacturing practices had design restrictions that prevented mimicking the natural architecture of human tissues into material manufacturing. However, with additive manufacturing, the material construction happens layer-by-layer over multiple axes simultaneously, thus enabling finer control over material placement, thereby overcoming the design challenge that prevented developing complex human architectures. This review substantiates the dexterity of additive manufacturing in utilizing biomimetics to 3D print ceramic, polymer, and metal implants with excellent resemblance to natural tissue. It also cites some clinical references of experimental and commercial approaches employing biomimetic 3D printing of implants. Full article

To understand the sustainability problem for Panama’s metropolitan area, its urban metabolism was investigated. A way to evaluate its current state was obtained by estimating a sustainable indicator based on the Green City Index. With the abstraction of the identified problems, the biomimetic strategy “problem-based approach” was carried out, where different pinnacles of nature were selected as a reference for the design of regenerative solutions. These were inspired by the understanding of the living world and how to include ecosystems in urban designs. Therefore, a framework was proposed for positive generation and natural solutions in cities to take advantage of the regenerative potential in Panama City. Using ecosystem services, a set of indicators were developed to measure regeneration over the years at the city scale. The results indicate that from the 11 selected pinnacles, 17 solutions inspired in nature were proposed to regenerate cities. Consequently, a SWOT analysis was realized along with a questionnaire by experts from different fields. The findings obtained show that the feasible solutions were: arborization, green facades, solar roofs, e-mobility, green corridors, bicycle lanes, sidewalks, and biofilters. This research represents a step towards creating and developing regenerative cities, thus improving the quality of life of living beings and ecosystems present in society. Full article

Quantifying movement variability is a crucial aspect for clinical and laboratory investigations in several contexts. However, very few studies have assessed, in detail, the intra-subject variability across movements and the inter-subject variability. Muscle synergies are a valuable method that can be used to assess such variability. In this study, we assess, in detail, intra-subject and inter-subject variability in a scenario based on a comprehensive dataset, including multiple repetitions of multi-directional reaching movements. The results show that muscle synergies are a valuable tool for quantifying variability at the muscle level and reveal that intra-subject variability is lower than inter-subject variability in synergy modules and related temporal coefficients, and both intra-subject and inter-subject similarity are higher than random synergy matching, confirming shared underlying control structures. The study deepens the available knowledge on muscle synergy-based motor function assessment and rehabilitation applications, discussing their applicability to real scenarios. Full article

Liver tissue engineering is a rapidly developing field which combines the novel use of liver cells, appropriate biochemical factors, and engineering principles, in order to replace or regenerate damaged liver tissue or the organ. The aim of this review paper is to critically investigate different possible methods to tackle issues related with liver diseases/disorders mainly using regenerative medicine. In this work the various regenerative treatment options are discussed, for improving the prognosis of chronic liver disorders. By reviewing existing literature, it is apparent that the current popular treatment option is liver transplantation, although the breakthroughs of stem cell-based therapy and bioartificial liver technology make them a promising alternative. Full article

Titanium has been successfully used in dental implantology for a long time. Due to the osseointegration process, titanium implants are able to withstand the chewing load. This article is devoted to the study of surface treatment methods of titanium alloys and the study of their interaction with mesenchymal stem cells (MSCs). The surface microrelief can influence MSC differentiation in different ways, which subsequently gives it osteogenic potential. The paper proposes modes of surface modification of titanium alloys on Grade 4 and Grade 1 by chemical and electrochemical (anodizing) etching. The possibility of modifying the surface of titanium alloys using the synthesis of graphene layers has been proposed in this paper for the first time. The osteogenic potential of a particular surface was assessed by the number of mesenchymal stem cells cultured on them under identical conditions. Full article

Construction materials and techniques have witnessed major advancements due to the application of digital tools in the design and fabrication processes, leading to a wide array of possibilities, especially in additive digital manufacturing tools and 3D printing techniques, scales, and materials. However, possibilities carry responsibilities with them and raise the question of the sustainability of 3D printing applications in the built environment in terms of material consumption and construction processes: how should one use digital design and 3D printing to achieve minimum material use, minimum production processes, and optimized application in the built environment? In this work, we propose an optimized formal design of “Biodigital Barcelona Clay Bricks” to achieve sustainability in the use of materials. These were achieved by using a bottom-up methodology of biolearning to extract the formal grammar of the bricks that is suitable for their various applications in the built environment as building units, thereby realizing the concept of formal physiology, as well as employing the concept of fractality or pixilation by using 3D printing to create the bricks as building units on an architectural scale. This enables the adoption of this method as an alternative construction procedure instead of conventional clay brick and full-scale 3D printing of architecture on a wider and more democratic scale, avoiding the high costs of 3D printing machines and lengthy processes of the one-step, 3D-printed, full-scale architecture, while also guaranteeing minimum material consumption and maximum forma–function coherency. The “Biodigital Barcelona Clay Bricks” were developed using Rhinoceros 3D and Grasshopper 3D + Plugins (Anemone and Kangaroo) and were 3D printed in clay. Full article

(1) Significance of geometry for bio-inspired hygroscopically actuated bilayer structures is well studied and can be used to fine-tune curvatures in many existent material systems. We developed a material design space to find new material combinations that takes into account unequal effective widths of the layers, as commonly used in fused filament fabrication, and deflections under self-weight. (2) For this purpose, we adapted Timoshenko’s model for the curvature of bilayer strips and used an established hygromorphic 4D-printed bilayer system to validate its ability to predict curvatures in various experiments. (3) The combination of curvature evaluation with simple, linear beam deflection calculations leads to an analytical solution space to study influences of Young’s moduli, swelling strains and densities on deflection under self-weight and curvature under hygroscopic swelling. It shows that the choice of the ratio of Young’s moduli can be crucial for achieving a solution that is stable against production errors. (4) Under the assumption of linear material behavior, the presented development of a material design space allows selection or design of a suited material combination for application-specific, bio-inspired bilayer systems with unequal layer widths. Full article

Worm-like robots have demonstrated great potential in navigating through environments requiring body shape deformation. Some examples include navigating within a network of pipes, crawling through rubble for search and rescue operations, and medical applications such as endoscopy and colonoscopy. In this work, we developed path planning optimization techniques and obstacle avoidance algorithms for the peristaltic method of locomotion of worm-like robots. Based on our previous path generation study using a modified rapidly exploring random tree (RRT), we have further introduced the Bézier curve to allow more path optimization flexibility. Using Bézier curves, the path planner can explore more areas and gain more flexibility to make the path smoother. We have calculated the obstacle avoidance limitations during turning tests for a six-segment robot with the developed path planning algorithm. Based on the results of our robot simulation, we determined a safe turning clearance distance with a six-body diameter between the robot and the obstacles. When the clearance is less than this value, additional methods such as backward locomotion may need to be applied for paths with high obstacle offset. Furthermore, for a worm-like robot, the paths of subsequent segments will be slightly different than the path of the head segment. Here, we show that as the number of segments increases, the differences between the head path and tail path increase, necessitating greater lateral clearance margins. Full article

Organisms and their features represent a complex system of solutions that can efficiently inspire the development of original and cutting-edge design applications: the related discipline is known as biomimetics. From the smallest to the largest, every species has developed and adapted different working principles based on their relative dimensional realm. In nature, size changes determine remarkable effects in organismal structures, functions, and evolutionary innovations. Similarly, size and scaling rules need to be considered in the biomimetic transfer of solutions to different dimensions, from nature to artefacts. The observation of principles that occur at very small scales, such as for nano- and microstructures, can often be seen and transferred to a macroscopic scale. However, this transfer is not always possible; numerous biological structures lose their functionality when applied to different scale dimensions. Hence, the evaluation of the effects and changes in scaling biological working principles to the final design dimension is crucial for the success of any biomimetic transfer process. This review intends to provide biologists and designers with an overview regarding scale-related principles in organismal design and their application to technical projects regarding mechanics, optics, electricity, and acoustics. Full article

This article proposes the integration of structural sizing, topology, and aerodynamic optimization for a morphing variable span of tapered wing (MVSTW) with the aim to minimize its weight. In order to evaluate the feasibility of the morphing wing optimization, this work creates a numerical environment by incorporating simultaneous structural sizing and topology optimization based on its aerodynamic analysis. This novel approach is proposed for an MVSTW. A problem-specific optimization approach to determine the minimum weight structure of the wing components for its fixed and moving segments is firstly presented. The optimization was performed using the OptiStruct solver inside HyperMesh. This investigation seeks to minimize total structure compliance while maximizing stiffness in order to satisfy the structural integrity requirements of the MVSTW. The aerodynamic load distribution along the wingspan at full wingspan extension and maximum speed were considered in the optimization processes. The wing components were optimized for size and topology, and all of them were built from aluminum alloy 2024-T3. The optimization results show that weight savings of up to 51.2% and 55.7% were obtained for fixed and moving wing segments, respectively. Based on these results, the optimized variable-span morphing wing can perform certain flight missions perfectly without experiencing any mechanical failures. Full article

This study aims to evaluates how an adaptive winglet during flight can improve aircraft aerodynamic characteristics of the CRJ700. The aircraft geometry was slightly modified to integrate a one-rotation axis adaptive winglet. Aerodynamic characteristics of the new adaptive design were computed using a validated high-fidelity aerodynamic model developed with the open-source code OpenFoam. The aerodynamic model successively uses the two solvers simpleFoam and rhoSimpleFoam based on Reynold Averaged Navier Stokes equations. Characteristics of the adaptive winglet design were studied for 16 flight conditions, representative of climb and cruise usually considered by the CRJ700. The adaptive winglet can increase the lift-to-drag ratio by up to 6.10% and reduce the drag coefficient by up to 2.65%. This study also compared the aerodynamic polar and pitching moment coefficients variations of the Bombardier CRJ700 equipped with an adaptive versus a fixed winglet. Full article