Natural Products: Phytochemical Extraction, Analysis and Application

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Agricultural Product Quality and Safety".

Deadline for manuscript submissions: closed (31 August 2024) | Viewed by 2333

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Department of Chromatographic Methods, Maria Curie-Skłodowska University, 20-031 Lublin, Poland
Interests: antioxidant properties; chromatography; sample preparation; food chemistry
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Special Issue Information

Dear Colleagues,

Phytochemicals are bioactive substances synthesized by plants to protect them against external factors. In addition, they determine color and aroma and influence the regulation of their growth. Phytochemicals also have other beneficial properties: bactericidal, fungicidal, insecticidal and antioxidant. The antioxidant properties are related to their chemical structure and are responsible for, among others, neutralizing free radicals, which means that they can directly affect the human body. Their protective and healing effects can be used to fight many diseases, including oxidative stress. Due to these properties, phytochemicals should be important components of the diet. Therefore, progress in techniques for the effective isolation of these compounds is very important. From traditional solvent extraction methods to innovative approaches, researchers continually strive to optimize extraction efficiency, selectivity and sustainability. By improving extraction techniques, researchers can effectively analyze these compounds qualitatively and quantitatively.

The aim of this Special Issue is to present the latest developments in phytochemical extraction techniques. By contributing to this Special Issue, you will contribute to the development of extraction methods, improving the identification, characterization and determination of phytochemicals for their effective use in human life. We look forward to your contributions and support for meaningful discussions in this important area of research.

Dr. Małgorzata Olszowy-Tomczyk
Guest Editor

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Keywords

  • natural compounds
  • phytochemistry
  • traditional extraction techniques
  • assisted extraction techniques
  • antioxidant compounds
  • chromatographic analysis

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Published Papers (2 papers)

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Research

13 pages, 1177 KiB  
Article
An In Vitro Study on the Antioxidant Properties of Cistus incanus Extracts
by Małgorzata Olszowy-Tomczyk and Dorota Wianowska
Agriculture 2024, 14(9), 1559; https://doi.org/10.3390/agriculture14091559 - 9 Sep 2024
Viewed by 729
Abstract
This paper concerns the evaluation of the antioxidant activity (AA) of extracts obtained from cistus herbs grown in Albania and Turkey. The extracts were prepared in a Soxhlet apparatus, as well as by the maceration and infusion methods, similar to the home method [...] Read more.
This paper concerns the evaluation of the antioxidant activity (AA) of extracts obtained from cistus herbs grown in Albania and Turkey. The extracts were prepared in a Soxhlet apparatus, as well as by the maceration and infusion methods, similar to the home method of preparing herbal teas. AA was determined using the DPPH (2,2′-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (ferric reducing antioxidant power) and β-carotene methods. It was proven that the AA of extracts depends not only on the extraction technique and AA assessment method but also on the place of plant cultivation. The smallest activity was determined using the β-carotene bleaching method, while the highest values were obtained using the FRAP method. On the other hand, the ABTS method showed a greater activity of the Albanian herb prepared using the Soxhlet technique. In addition, the antioxidant properties of extracts were compared with those of rutin standard solutions, showing that this characteristic component of cistus is not the only one that determines AA of extracts. As a result, the rutin content is not an indicator of the antioxidant properties of extracts. The other polyphenolic compounds, although occurring at lower concentration levels compared to rutin, modify the resultant AA of extracts. These studies confirmed the biological activity of cistus as a valuable source of polyphenolic compounds in the human diet. Full article
(This article belongs to the Special Issue Natural Products: Phytochemical Extraction, Analysis and Application)
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12 pages, 1072 KiB  
Article
Improvement in Natural Antioxidant Recovery from Sea Buckthorn Berries Using Predictive Model-Based Optimization
by Seunghee Kim, Jeongho Lee, Hyerim Son, Kang Hyun Lee, Youngsang Chun, Ja Hyun Lee, Taek Lee and Hah Young Yoo
Agriculture 2024, 14(7), 1095; https://doi.org/10.3390/agriculture14071095 - 7 Jul 2024
Viewed by 1154
Abstract
Sea buckthorn berries (SBB) are well known for being rich in natural bioactive compounds with high pharmacological activity. In this study, the optimization of extraction parameters was performed to recover phenolic compounds with high antioxidant activity from SBB. This study involved a systematic [...] Read more.
Sea buckthorn berries (SBB) are well known for being rich in natural bioactive compounds with high pharmacological activity. In this study, the optimization of extraction parameters was performed to recover phenolic compounds with high antioxidant activity from SBB. This study involved a systematic optimization approach, including screening for a variety of parameters, including temperature, time, ethanol concentration, agitation, and solid loading. On the basis of Plackett–Burman design (PBD) model, the two most significant parameters (agitation and solid loading) were selected, and the correlation model between those parameters and multiple responses was derived via response surface methodology (RSM). As a result, the optimal extraction condition for maximizing phenolic content and antioxidant activity was determined to be agitation at 109.54 rpm and a solid loading of 172.67 g/L. Under optimal conditions, SBB extract showed a total phenolic content of 0.21 mg/mL and ABTS and DPPH activities of 27.27% and 58.16%, respectively. The SBB extract prepared under optimal conditions was found to contain caffeic acid, vanillic acid, rutin, and vitamin B1 (thiamine). This work is the first challenge to design an optimization model for the efficient recovery of antioxidants from SBB and is significant in that the model can be applied simply and economically to conventional extraction processes. Full article
(This article belongs to the Special Issue Natural Products: Phytochemical Extraction, Analysis and Application)
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