Novel Techniques for Analysis and Determination of Mycotoxins in Food

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Food Science and Technology".

Deadline for manuscript submissions: closed (20 November 2022) | Viewed by 2156

Special Issue Editor


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Guest Editor
Department of Preventive Medicine and Public Health, Food Sciences, Forensic Medicine and Toxicology, University of Valencia | UV, E-46100 Valencia, Spain
Interests: food safety; mass spectrometry analysis; proteomics; risk assessment; toxicology
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Special Issue Information

Dear Colleagues,

Mycotoxins contaminate crops worldwide and can play a role in animal health and performance, and crucial importance for ensuring food safety and quality. Furthermore, multiple mycotoxins may be present together in food and feeds which could increase the negative impacts on the human and animal. A highly sensitive system for the accurate analysis of mycotoxins are of paramount importance. So that novel techniques to assess the occurrence of mycotoxins in food and feed are necessary to successfully detect and identify important (legislated and none legislated) mycotoxins. Some of them have proven to be particularly effective in not only the detection of mycotoxins, but also in detecting mycotoxin-producing fungi such as masked mycotoxins. Chromatographic techniques such as high-performance liquid chromatography coupled with various detectors like fluorescence, diode array, UV, liquid chromatography coupled with mass spectrometry, liquid chromatography-tandem mass spectrometry and liquid chromatography coupled with time of flight-mass spectrometry have been powerful tools for analyzing and detecting major mycotoxins. Recent progress of the development of rapid immunoaffinity-based detection techniques such as immunoassays and biosensors, as well as emerging technologies like proteomic and genomic methods, molecular techniques, electronic nose, aggregation-induced emission dye, quantitative NMR and hyperspectral imaging for the detection of mycotoxins in foods, are also welcoming. Novelties in extraction, purification and isolation, including some derivatization are interesting improvement strategies of analysis.

Prof. Dr. Cristina Juan García
Guest Editor

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Keywords

  • mycotoxin in food
  • detection techniques
  • determination
  • analysis
  • food safety
  • food quality
  • chromatographic techniques

Published Papers (1 paper)

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Research

15 pages, 4045 KiB  
Article
Multimycotoxin Analysis in Oat, Rice, Almond and Soy Beverages by Liquid Chromatography-Tandem Mass Spectrometry
by Cristina Juan, Jordi Mañes, Ana Juan-García and Juan Carlos Moltó
Appl. Sci. 2022, 12(8), 3942; https://doi.org/10.3390/app12083942 - 13 Apr 2022
Cited by 7 | Viewed by 1687
Abstract
This study developed and validated an analytical methodology for the determination of aflatoxins, enniatins, beauvericin, zearalenone, ochratoxin-A, alternariols, HT-2 and T-2 toxin in soy, oat, rice and almond beverages, based on solid phase extraction columns (SPE) and analyzed by liquid chromatography coupled to [...] Read more.
This study developed and validated an analytical methodology for the determination of aflatoxins, enniatins, beauvericin, zearalenone, ochratoxin-A, alternariols, HT-2 and T-2 toxin in soy, oat, rice and almond beverages, based on solid phase extraction columns (SPE) and analyzed by liquid chromatography coupled to mass spectrometry in tandem. C18 SPE was successfully applied, obtaining recoveries that range from 72 ± 12% (ochratoxin-A) to 99 ± 4% (ENA1) at high level (L1) and 65 ± 8% (T-2) to 128 ± 9% (alternariol monomethyl ether) at low levels (L3). The methodology was validated according to Commission Decision 2002/657/EC, with limits of quantification ranging from 0.3 (AFs in oat beverages) to 18 ng/mL (HT-2 in rice beverage). The analysis of 56 beverage samples purchased from Valencia (Spain) showed at least one mycotoxin occurring in 95% of samples, including carcinogenic aflatoxins, and oat beverage was the most contaminated. This is a newest validated methodology for the quantification of sixty mycotoxins in oat, rice, almond and soy beverages. Full article
(This article belongs to the Special Issue Novel Techniques for Analysis and Determination of Mycotoxins in Food)
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