Bioengineering

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Special Issue Editors

Dr. Sabine Kleinsteuber

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Guest Editor

Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany
Interests: environmental microbiology; microbial ecology; biotechnology; anaerobic microbes; microbial communities; molecular methods; anaerobic digestion; fermentation pathways; metagenomics; biodegradation
Special Issues, Collections and Topics in MDPI journals

Prof. Dr. Susan Sharfstein

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College of Nanoscale Science and Engineering, The State University of New York Polytechnic Institute, Albany, NY 12203, USA
Interests: recombinant protein production; monoclonal antibodies; Chinese hamster ovary cells; omics, biomanufacturing; biosimilars; protein glycosylation; bioreactors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is part of the MDPI journal initiative to promote and support the contributions of women in research. We cordially invite researchers to submit their work on topics across all areas of “Biochemical Engineering”. Both original research articles and comprehensive review papers are welcome.

For this Special Issue, we welcome all research led by female scientists, where male scientists may offer support for the initiative as co-authors. We welcome submissions from all authors, irrespective of gender.

Dr. Sabine Kleinsteuber
Prof. Dr. Susan Sharfstein
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Women’s Special Issue Series

This Special Issue is part of Bioengineering's Women’s Special Issue Series, hosted by women editors for women researchers. The Series advocates the advancement of women in science. We invite contributions to the Special Issue whose lead authors identify as women. The submission of articles with all-women authorship is especially encouraged. However, we do welcome articles from all authors, irrespective of gender.

Keywords

biotechnology
biofuels and biorefinery
pharmaceuticals
water purification and food

Published Papers (2 papers)

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Research

14 pages, 2143 KiB

Open AccessArticle

Investigating the Processing Potential of Ethiopian Agricultural Residue Enset/Ensete ventricosum for Biobutanol Production

by Nebyat Seid, Pia Griesheimer and Anke Neumann

Bioengineering 2022, 9(4), 133; https://doi.org/10.3390/bioengineering9040133 - 24 Mar 2022

Cited by 4 | Viewed by 2812

Abstract

The Enset plant is a potential food source for about 20 million Ethiopians. A massive amount of residual byproduct is discarded from traditional Ethiopian Enset food processing. This study shows a compositional analysis of Enset biomass and its use for biobutanol production. The Enset biomass was pretreated with 2% (w/v) NaOH or 2% (v/v) H₂SO₄ and subjected to enzymatic hydrolysis. The enzymatic hydrolysates were then fermented anaerobically by C. saccharoperbutylacetonicum DSM 14923. The majority of Enset biomass waste samples contained 36–67% cellulose, 16–20% hemicelluloses, and less than 6.8% lignin. In all alkali-pretreated Enset biomass samples, the enzyme converted 80–90% of the biomass to glucose within 24 h, while it took 60 h to convert 48–80% of the acid-pretreated Enset biomass. In addition, the alkali pretreatment method released more glucose than the acid pretreatment in all Enset biomass samples. After 72 h of ABE fermentation, 2.8 g/L acetone, 9.9 g/L butanol, and 1.6 g/L ethanol were produced from mixed Enset waste hydrolysate pretreated with alkali, achieving an ABE yield of 0.32 g/g and productivity of 0.2 g × L⁻¹ × h⁻¹, showing the first value of butanol produced from Enset biomass in the literature. Full article

(This article belongs to the Special Issue Women's Special Issue Series: Biochemical Engineering)

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Graphical abstract

13 pages, 11648 KiB

Open AccessArticle

Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins

by Seçkin Akgül, Carolin Offenhäuser, Anja Kordowski and Bryan W. Day

Bioengineering 2022, 9(3), 91; https://doi.org/10.3390/bioengineering9030091 - 25 Feb 2022

Viewed by 3401

Abstract

Lentiviral vectors are unique and highly efficient genetic tools to incorporate genetic materials into the genome of a variety of cells whilst conserving biosafety. Their rapid acceptance made it necessary to improve existing protocols, including molecular engineering and cloning, production of purified lentiviral particles, and efficient infection of target cells. In addition to traditional protocols, which can be time-consuming, several biotechnology companies are providing scientists with commercially available lentiviral constructs and particles. However, these constructs are limited by their original form, tend to be costly, and lack the flexibility to re-engineer based on the ever-changing needs of scientific projects. Therefore, the current study organizes the existing methods and integrates them with novel ideas to establish a protocol that is simple and efficient to implement. In this study we, (i) generated an innovative site-directed nucleotide attachment/replacement and DNA insertion method using unique PCR primers, (ii) improved traditional methods by integrating plasmid clarification steps, (iii) utilized endogenous mRNA as a resource to construct new lentiviruses, and (iv) identified an existing purification method and incorporated it into an organized workflow to produce high-yield lentiviral particle collection. Finally, (v) we verified and demonstrated the functional validity of our methods using an infection strategy. Full article

(This article belongs to the Special Issue Women's Special Issue Series: Biochemical Engineering)

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