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Bioengineering
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Advances in Microseparation Technology
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Advances in Microseparation Technology

A special issue of Bioengineering (ISSN 2306-5354).

Deadline for manuscript submissions: closed (30 September 2018) | Viewed by 9971

Special Issue Editor

Prof. Dr. Jochen Strube

E-Mail Website
Guest Editor
Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstr. 15, D-38678 Clausthal-Zellerfeld, Germany
Interests: biologics and botanical manufacturing technology; green technology; digital twins and process analytical technology under quality by design
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues

Needs in novel white and red biotechnology process applications, which are derived from large social needs related to, e.g., the bio-based world and innovative medicine initiatives, are causing new challenging separation tasks of high volume/low cost, as well as low volume/high value products at a reduced cost-of-goods. Therefore, improvements in efficient downstream processing technologies for biotechnology process development and manufacturing are still key-issues under research and development. Process intensification focuses on process integration using consistent design methods for hybrid separations and optimal total process integration methods, based on process modeling, as well as process enhancement activities, such as micro-/milli-structured devices, ultra-sound, microwave, pulsed-electric-field enhances mass transfer and/or novel separation media, e.g., nano-scaled and/or selectivity/capacity enhanced.

Recently, micro-/milli-structured devices have been invented and characterized for different process applications, such as chromatography, membranes, precipitation/crystallization, as well as liquid–liquid extraction and distillation. Novel media have been introduced and mass transfer enhancing energy input described.

Nevertheless, further activities are needed to make design methods, materials and devices more applicable for the community in order to speed up industrialization. Especially in regulated industries, any change in process development and manufacturing technology has to be convincing for regulatory authorities and, therefore, applicants based on sound scientific methods related to consistent transparent data-driven decisions.

This Special Issue will help to bridge the gap between fundamental academia research and industrial utilization. Therefore, any contributions related to the above-mentioned topics and applications are welcome.

Prof. Dr. Jochen Strube
Guest Editor

The first round submission deadline: 30 September 2017

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Biotechnology
  • Process Intensification
  • Micro Technology
  • Downstream Processing

Published Papers (1 paper)

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Research

1915 KiB  
Article
Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells
by Yuxi Sun and Palaniappan Sethu
Bioengineering 2017, 4(3), 67; https://doi.org/10.3390/bioengineering4030067 - 02 Aug 2017
Cited by 16 | Viewed by 9537
Abstract
Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer [...] Read more.
Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer as a narrow band between the density-gradient medium and platelet-rich plasma. We hypothesized that microfluidic adaptation of this technique could transform this process into a rapid fractionation approach where samples are separated in a continuous fashion while being exposed to lower levels of stress (<100 g) for shorter durations of time (<3 min). To demonstrate proof-of-concept, we designed a microfluidic density-gradient centrifugation device and constructed a setup to introduce samples and medium like Ficoll in a continuous, pump-less fashion where cells and particles can be exposed to centrifugal force and separated via different outlets. Proof-of-concept studies using binary mixtures of low-density polystyrene beads (1.02 g/cm3) and high-density silicon dioxide beads (2.2 g/cm3) with Ficoll–Paque (1.06 g/cm3) show that separation is indeed feasible with >99% separation efficiency suggesting that this approach can be further adapted for separation of cells. Full article
(This article belongs to the Special Issue Advances in Microseparation Technology)
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