Fluorescent Sensors for Biological Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: closed (14 April 2024) | Viewed by 1493

Special Issue Editors


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Guest Editor
Department of Chemistry, University of Bath, Bath BA2 7AY, UK
Interests: fluorescent sensor design; molecular recognition; theranostic systems; probes for redox imbalance

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Guest Editor
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Interests: enzymatic biosensors; optical substrates; hydrolases; cytochrome P450 enzymes; transferases
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Special Issue Information

Dear Colleagues,

The past few decades have witnessed extraordinary advances in fluorescent sensors that have revolutionized the way biology can be studied. Fluorescent sensors are usually based on fluorescent organic molecules, nanoparticles, proteins, or combinations of organic molecules, nanoparticles, and proteins. They are designed and engineered to change their fluorescent colors or intensities in response to external stimuli or physiological changes, including pH fluctuations, metal ion homeostasis, cell signaling, membrane potential differences, phosphorylation, ubiquitination, redox reactions, and apoptosis.

In particular, the 2008 Nobel Prize in Chemistry was awarded to three scientists who discovered and developed fluorescent proteins, which have now been developed into a very large group of biosensors.

This Special Issue will focus on the use of fluorescent sensors in biological applications through an interdisciplinary approach. Our goal is to bring together researchers active in the development of innovative optically activated materials for the advancement of biophotonics. Both review articles and original research papers are welcome. There is a particular interest in papers concerning new biomedicine frontiers that may be surpassed through fluorescence imaging or sensing.

We look forward to your submissions.

Dr. Luling Wu
Prof. Dr. Guang-Bo Ge
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescent probes
  • fluorescence imaging
  • fluorescent labeling
  • multiple-responsive
  • fluorescent prodrugs

Published Papers (1 paper)

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Research

13 pages, 2871 KiB  
Article
A Ratiometric Biosensor Containing Manganese Dioxide Nanosheets and Nitrogen-Doped Quantum Dots for 2,4-Dichlorophenoxyacetic Acid Monitoring
by Yang Guo, Jingran Sun, Mingzhu Liu, Jin Wu, Zunquan Zhao, Ting Ma and Yanjun Fang
Biosensors 2024, 14(2), 63; https://doi.org/10.3390/bios14020063 - 24 Jan 2024
Viewed by 1177
Abstract
Nanomaterials are desirable for sensing applications. Therefore, MnO2 nanosheets and nitrogen-doped carbon dots (NCDs) were used to construct a ratiometric biosensor for quantification of 2,4-dichlorophenoxyacetic acid. The MnO2 nanosheets drove the oxidation of colorless o-phenylenediamine to OPDox, which exhibits fluorescence emission [...] Read more.
Nanomaterials are desirable for sensing applications. Therefore, MnO2 nanosheets and nitrogen-doped carbon dots (NCDs) were used to construct a ratiometric biosensor for quantification of 2,4-dichlorophenoxyacetic acid. The MnO2 nanosheets drove the oxidation of colorless o-phenylenediamine to OPDox, which exhibits fluorescence emission peaks at 556 nm. The fluorescence of OPDox was efficiently quenched and the NCDs were recovered as the ascorbic acid produced by the hydrolyzed alkaline phosphatase (ALP) substrate increased. Owing to the selective inhibition of ALP activity by 2,4-D and the inner filter effect, the fluorescence intensity of the NCDs at 430 nm was suppressed, whereas that at 556 nm was maintained. The fluorescence intensity ratio was used for quantitative detection. The linear equation was F = 0.138 + 3.863·C 2,4-D (correlation coefficient R2 = 0.9904), whereas the limits of detection (LOD) and quantification (LOQ) were 0.013 and 0.040 μg/mL. The method was successfully employed for the determination of 2,4-D in different vegetables with recoveries of 79%~105%. The fluorescent color change in the 2,4-D sensing system can also be captured by a smartphone to achieve colorimetric detection by homemade portable test kit. Full article
(This article belongs to the Special Issue Fluorescent Sensors for Biological Applications)
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