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Biosensors
Special Issues
Development of Novel Biosensors for Point-of-Care Detection
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Development of Novel Biosensors for Point-of-Care Detection

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 4967

Special Issue Editors

Dr. Jiasi Wang

E-Mail Website
Guest Editor
School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen, China
Interests: digital PCR; POCT; single-moleclue detection
Dr. Lu Huang

E-Mail Website
Guest Editor
School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, China
Interests: microfluidic chip; single-cell analysis; high-throughput technology
Dr. Xinming Huo

E-Mail Website
Guest Editor
School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, China
Interests: Portable in-situ detection devices and instruments; MEMS sensors

Special Issue Information

Dear Colleagues,

Novel infectious pathogens such as SARS-CoV2 present a tremendous threat to human society. Traditional detection methods based on sophisticated instrumentation in central labs are time-consuming and require trained personnel. There is an urgent need for biosensors that can rapidly and accurately detect pathogens at the point-of-care (PoC) to improve responsiveness to outbreaks. PoC diagnostic devices could also be used in human healthcare testing and food/environmental monitoring in low-resource settings. There has been remarkable progress in PoC biosensors in recent years in terms of miniaturization, multiplex analysis, and readout signal enhancement, thus improving analytical performance. The advancing field of diagnostic devices integrating microfluidics, nanomaterials, optics, and electronics offers new possibilities for the rapid, low-cost detection of various pathogens and biomarkers. This Special Issue will present the latest developments in PoC biosensors for applications. Topics of interest include but are not limited to the following:

  • Low-cost portable devices for in situ measurement;
  • “Lab-on-a-chip” sensors;
  • Nanomaterials-based biosensors;
  • Paper-based biosensors;
  • Wearable biosensors.

Dr. Jiasi Wang
Dr. Lu Huang
Dr. Xinming Huo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • POCT
  • biosensor
  • lab-on-chip
  • low-cost portable devices

Published Papers (4 papers)

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Research

14 pages, 3096 KiB  
Article
Real-Time Monitoring of a Nucleic Acid Amplification Reaction Using a Mass Sensor Based on a Quartz-Crystal Microbalance
by Hideto Kumagai and Hiroyuki Furusawa
Biosensors 2024, 14(4), 155; https://doi.org/10.3390/bios14040155 - 25 Mar 2024
Viewed by 879
Abstract
Nucleic acid amplification reactions such as polymerase chain reaction (PCR), which uses a DNA polymerase to amplify individual double-stranded DNA fragments, are a useful technique for visualizing the presence of specific genomes. Although the fluorescent labeling method is mainly used with DNA amplification, [...] Read more.
Nucleic acid amplification reactions such as polymerase chain reaction (PCR), which uses a DNA polymerase to amplify individual double-stranded DNA fragments, are a useful technique for visualizing the presence of specific genomes. Although the fluorescent labeling method is mainly used with DNA amplification, other detection methods should be considered for further improvements, such as miniaturization and cost reduction, of reaction-monitoring devices. In this study, the quartz-crystal microbalance (QCM) method, which can measure nanogram-order masses, was applied for the real-time detection of DNA fragments in a solution with nucleic acids. This was combined with an isothermal nucleic acid amplification reaction based on the recombinase polymerase amplification (RPA) method, which allowed DNA amplification at a constant temperature. When the DNA amplification reaction was initiated on a QCM sensor plate with an immobilized primer DNA strand, a significant increase in mass was observed compared to when the primer DNA was not immobilized. QCM was shown to be sufficiently sensitive for the in situ detection of amplified DNA fragments. Combining a portable QCM device and RPA offers a sensitive point-of-care method for detecting nucleic acids. Full article
(This article belongs to the Special Issue Development of Novel Biosensors for Point-of-Care Detection)
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22 pages, 9533 KiB  
Article
Microwave Imaging System Based on Signal Analysis in a Planar Environment for Detection of Abdominal Aortic Aneurysms
by Andrea Martínez-Lozano, Roberto Gutierrez, Carlos G. Juan, Carolina Blanco-Angulo, Héctor García-Martínez, Germán Torregrosa, José María Sabater-Navarro and Ernesto Ávila-Navarro
Biosensors 2024, 14(3), 149; https://doi.org/10.3390/bios14030149 - 18 Mar 2024
Viewed by 999
Abstract
A proof-of-concept of a microwave imaging system for the fast detection of abdominal aortic aneurysms is shown. This experimental technology seeks to overcome the factors hampering the fast screening for these aneurysms with the usual equipment, such as high cost, long-time operation or [...] Read more.
A proof-of-concept of a microwave imaging system for the fast detection of abdominal aortic aneurysms is shown. This experimental technology seeks to overcome the factors hampering the fast screening for these aneurysms with the usual equipment, such as high cost, long-time operation or hazardous exposure to chemical substances. The hardware system is composed of 16 twin antennas mastered by a microcontroller through a switching network, which connects the antennas to the measurement instrument for sequential measurement. The software system is run by a computer, mastering the whole system, automatizing the measurement process and running the signal processing and medical image generation algorithms. Two image generation algorithms are tested: Delay-and-Sum (DAS) and Improved Delay-and-Sum (IDAS). Own-modified versions of these algorithms adapted to the requirements of our system are proposed. The system is carefully calibrated and fine-tuned with known objects placed at known distances. An experimental proof-of-concept is shown with a human torso phantom, including an aorta phantom and an aneurysm phantom placed in different positions. The results show good imaging capabilities with the potential for detecting and locating possible abdominal aortic aneurysms and reporting acceptable errors. Full article
(This article belongs to the Special Issue Development of Novel Biosensors for Point-of-Care Detection)
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14 pages, 3894 KiB  
Article
Mill Scale-Derived Magnetite Nanoparticles: A Novel Substrate for Lactate Oxidase-Based Biosensors
by Hamid Khosravi, Oscar Carreras-Gallo and Jasmina Casals-Terré
Biosensors 2023, 13(11), 957; https://doi.org/10.3390/bios13110957 - 27 Oct 2023
Cited by 1 | Viewed by 1295
Abstract
Recycling and revalorization of waste are currently essential for sustainable growth. Mill scale, a waste product from steel production industries, which contains high levels of iron and minimal impurities, is proposed in this study as the source to synthesize magnetite nanoparticles (Fe3 [...] Read more.
Recycling and revalorization of waste are currently essential for sustainable growth. Mill scale, a waste product from steel production industries, which contains high levels of iron and minimal impurities, is proposed in this study as the source to synthesize magnetite nanoparticles (Fe3O4) for an enhancement of a lactate biosensor range. The synthesized Fe3O4 nanoparticles were coated with polydopamine (PDA) to prevent aggregation and degradation, creating a stable platform for immobilizing lactate oxidase enzyme (LOx) on their surfaces. The characterization of the Fe3O4@PDA material was carried out using transmission electron microscopy (TEM), dynamic light scattering (DLS), and measurement of the polydispersity index (PdI). The Fe3O4@PDA-LOx material was then deposited on a screen-printed carbon electrode modified with Prussian blue (SPCE-PB) for lactate detection. The biosensor exhibited a broad, dual linear concentration-response range, one from 0.1 to 4.62 mM with a limit of detection of 0.32 mM and sensitivity of 1.54 μAmM−1cm−2, and another one from 4.62 to 149.21 mM with a limit of detection of 6.31 mM and sensitivity of 0.08 μAmM−1cm−2. The dual-range concentration response of the biosensor makes it an ideal tool for lactate determination in various applications, including sports medicine, clinical diagnosis, and industrial bioprocessing. Full article
(This article belongs to the Special Issue Development of Novel Biosensors for Point-of-Care Detection)
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10 pages, 2244 KiB  
Communication
Rapid On-Site Detection of SARS-CoV-2 Using RT-LAMP Assay with a Portable Low-Cost Device
by Quanying Fu, Xueyuan Pang, Zhenning Su, Yuxiao Yang, Yiren Liu, Ziyue Zhang, Yuqiu Fu, Jiasi Wang and Jianhua Zhou
Biosensors 2023, 13(7), 724; https://doi.org/10.3390/bios13070724 - 12 Jul 2023
Viewed by 1377
Abstract
Emerging infectious diseases pose a serious threat to human health and affect social stability. In recent years, the epidemic situation of emerging infectious diseases is very serious; among these infectious diseases, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected many countries and [...] Read more.
Emerging infectious diseases pose a serious threat to human health and affect social stability. In recent years, the epidemic situation of emerging infectious diseases is very serious; among these infectious diseases, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected many countries and regions in a short time. The prevention and treatment of these diseases require rapid on-site detection methods. However, the common detection method, RT-PCR, requires expensive instruments, complex operations, and professional operators. Here, we developed a portable low-cost assay for rapid on-site detection of viral nucleic acid using reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The SARS-CoV-2 RNA can be successfully amplified within 15 min in a thermos, and the detection result is read rapidly in a portable low-cost device with a sensitivity of 100 copies/µL. The portable low-cost device consists of a black box, a laser or LED and a filter, costing only a few cents. The rapid on-site detection method can provide strong support for the control of biological threats such as infectious diseases. It is also an emergency detection method for low-resource settings, relieving the huge pressure on health care. Full article
(This article belongs to the Special Issue Development of Novel Biosensors for Point-of-Care Detection)
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