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Biosensors
Special Issues
Electrochemical Based Biosensors
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Electrochemical Based Biosensors

A special issue of Biosensors (ISSN 2079-6374).

Deadline for manuscript submissions: closed (30 April 2012) | Viewed by 73760

Special Issue Editor

Prof. Dr. Chung-Chiun Liu

E-Mail Website
Guest Editor
Department of Chemical and Biomolecular Engineering and Electronics Design Center, Case Western Reserve University, Cleveland, OH 44106, USA
Interests: electrochemical-based chemical and bio-sensors; microfabrication technology; nano-catalysts and sensing meterials; CRISPR-related biosensing strategy; bio-conjugation technology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Electrochemical based biosensors have been used extensively in various laboratory, clinical, home care, and point-of-care applications. Nano-catalysts, microfabrication manufacturing processing, the advancement of computer sciences and electronic technology, as well as the identification of newly discovered biomarkers have led the advancement of electrochemical based biosensors to new dimensions.

This special issue invites contributions particularly relating to application-oriented electrochemical based biosensors using innovative development techniques. Examples of application areas include, but are not limited to, different methods of electrochemical detection which can be applied to useful biosensing applications. Many enzymatic reactions produce electrochemically active species such as H2O2, NADH and others which can be used as a foundation to monitor various analytes (biomarkers). Microfabrication processing, including thick- and thin-film techniques, are often employed in the fabrication of biosensor prototypes, and the electrochemical-based biosensors that are produced provide new means in biosensing. Extension of these biosensors to real time, single use, disposable point of care biosensors and minute in vivo biosensors will be of interest to the readers of this special issue.

Prof. Dr. Chung-Chiun Liu
Guest Editor

Keywords

  • electrochemical based biosensors
  • microfabication
  • nano-catalysts
  • in vitro biosensors
  • in vivo biosensors
  • enzymes
  • H2O2
  • NADH

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Published Papers (7 papers)

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Editorial

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26 KiB  
Editorial
Electrochemical Based Biosensors
by Chung Chiun Liu
Biosensors 2012, 2(3), 269-272; https://doi.org/10.3390/bios2030269 - 24 Jul 2012
Cited by 18 | Viewed by 7058
Abstract
This editorial summarizes the general approaches of the electrochemical based biosensors described in the manuscripts published in this Special Issue. Electrochemical based biosensors are scientifically and economically important for the detection and early diagnosis of many diseases, and they will be increasing used [...] Read more.
This editorial summarizes the general approaches of the electrochemical based biosensors described in the manuscripts published in this Special Issue. Electrochemical based biosensors are scientifically and economically important for the detection and early diagnosis of many diseases, and they will be increasing used and developed in the coming years. The importance of the selection of recognition processes, fabrication techniques and biosensor materials will be introduced. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)

Research

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378 KiB  
Article
Effect of Toxic Components on Microbial Fuel Cell-Polarization Curves and Estimation of the Type of Toxic Inhibition
by Nienke E. Stein, Hubertus V. M. Hamelers, Gerrit Van Straten and Karel J. Keesman
Biosensors 2012, 2(3), 255-268; https://doi.org/10.3390/bios2030255 - 11 Jul 2012
Cited by 36 | Viewed by 9497
Abstract
Polarization curves are of paramount importance for the detection of toxic components in microbial fuel cell (MFC) based biosensors. In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl [...] Read more.
Polarization curves are of paramount importance for the detection of toxic components in microbial fuel cell (MFC) based biosensors. In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide. The experimental polarization curves show that toxic components have an effect on the electrochemically active bacteria in the cell. (Extended) Butler Volmer Monod (BVM) models were used to describe the polarization curves of the MFC under nontoxic and toxic conditions. It was possible to properly fit the (extended) BVM models using linear regression techniques to the polarization curves and to distinguish between different types of kinetic inhibitions. For each of the toxic components, the value of the kinetic inhibition constant Ki was also estimated from the experimental data. The value of Ki indicates the sensitivity of the sensor for a specific component and thus can be used for the selection of the biosensor for a toxic component. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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397 KiB  
Article
Peroxide-Dependent Analyte Conversion by the Heme Prosthetic Group, the Heme Peptide “Microperoxidase-11” and Cytochrome c on Chitosan Capped Gold Nanoparticles Modified Electrodes
by Aysu Yarman, Bettina Neumann, Maria Bosserdt, Nenad Gajovic-Eichelmann and Frieder W. Scheller
Biosensors 2012, 2(2), 189-204; https://doi.org/10.3390/bios2020189 - 14 May 2012
Cited by 13 | Viewed by 9483
Abstract
In view of the role ascribed to the peroxidatic activity of degradation products of cytochrome c (cyt c) in the processes of apoptosis, we investigate the catalytic potential of heme and of the cyt c derived heme peptide MP-11 to catalyse the cathodic [...] Read more.
In view of the role ascribed to the peroxidatic activity of degradation products of cytochrome c (cyt c) in the processes of apoptosis, we investigate the catalytic potential of heme and of the cyt c derived heme peptide MP-11 to catalyse the cathodic reduction of hydrogen peroxide and to oxidize aromatic compounds. In order to check whether cyt c has an enzymatic activity in the native state where the protein matrix should suppress the inherent peroxidatic activity of its heme prosthetic group, we applied a biocompatible immobilization matrix and very low concentrations of the co-substrate H2O2. The biocatalysts were entrapped on the surface of a glassy carbon electrode in a biocompatible chitosan layer which contained gold nanoparticles. The electrochemical signal for the peroxide reduction is generated by the redox conversion of the heme group, whilst a reaction product of the substrate oxidation is cathodically reduced in the substrate indication. The catalytic efficiency of microperoxidase-11 is sufficient for sensors indicating HRP substrates, e.g., p-aminophenol, paracetamol and catechol, but also the hydroxylation of aniline and dehalogenation of 4-fluoroaniline. The lower limit of detection for p-aminophenol is comparable to previously published papers with different enzyme systems. The peroxidatic activity of cyt c immobilized in the chitosan layer for catechol was found to be below 1 per mill and for p-aminophenol about 3% as compared with that of heme or MP-11. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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1025 KiB  
Article
Enzyme-Gelatin Electrochemical Biosensors: Scaling Down
by Karolien De Wael, Stijn De Belder, Sanaz Pilehvar, Geert Van Steenberge, Wouter Herrebout and Hendrik A. Heering
Biosensors 2012, 2(1), 101-113; https://doi.org/10.3390/bios2010101 - 15 Mar 2012
Cited by 18 | Viewed by 7758
Abstract
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a [...] Read more.
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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293 KiB  
Article
Poly(lactic acid)/Carbon Nanotube Fibers as Novel Platforms for Glucose Biosensors
by Juliano Elvis Oliveira, Luiz Henrique Capparelli Mattoso, Eliton Souto Medeiros and Valtencir Zucolotto
Biosensors 2012, 2(1), 70-82; https://doi.org/10.3390/bios2010070 - 27 Feb 2012
Cited by 42 | Viewed by 9306
Abstract
The focus of this paper is the development and investigation of properties of new nanostructured architecture for biosensors applications. Highly porous nanocomposite fibers were developed for use as active materials in biosensors. The nanocomposites comprised poly(lactic acid)(PLA)/multi-walled carbon nanotube (MWCNT) fibers obtained via [...] Read more.
The focus of this paper is the development and investigation of properties of new nanostructured architecture for biosensors applications. Highly porous nanocomposite fibers were developed for use as active materials in biosensors. The nanocomposites comprised poly(lactic acid)(PLA)/multi-walled carbon nanotube (MWCNT) fibers obtained via solution-blow spinning onto indium tin oxide (ITO) electrodes. The electrocatalytic properties of nanocomposite-modified ITO electrodes were investigated toward hydrogen peroxide (H2O2) detection. We investigated the effect of carbon nanotube concentration and the time deposition of fibers on the sensors properties, viz., sensitivity and limit of detection. Cyclic voltammetry experiments revealed that the nanocomposite-modified electrodes displayed enhanced activity in the electrochemical reduction of H2O2, which offers a number of attractive features to be explored in development of an amperometric biosensor. Glucose oxidase (GOD) was further immobilized by drop coating on an optimized ITO electrode covered by poly(lactic acid)/carbon nanotube nanofibrous mats. The optimum biosensor response was linear up to 800 mM of glucose with a sensitivity of 358 nA·mM−1 and a Michaelis-Menten constant (KM) of 4.3 mM. These results demonstrate that the solution blow spun nanocomposite fibers have great potential for application as amperometric biosensors due to their high surface to volume ratio, high porosity and permeability of the substrate. The latter features may significantly enhance the field of glucose biosensors. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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425 KiB  
Article
ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin
by Zafar Hussain Ibupoto, Syed Muhammad Usman Ali, Kimleang Khun, Chan Oeurn Chey, Omer Nur and Magnus Willander
Biosensors 2011, 1(4), 153-163; https://doi.org/10.3390/bios1040153 - 27 Oct 2011
Cited by 38 | Viewed by 10009
Abstract
In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized [...] Read more.
In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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Review

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3586 KiB  
Review
Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro
by Jun Wang, Chengxiong Wu, Ning Hu, Jie Zhou, Liping Du and Ping Wang
Biosensors 2012, 2(2), 127-170; https://doi.org/10.3390/bios2020127 - 25 Apr 2012
Cited by 54 | Viewed by 19668
Abstract
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the [...] Read more.
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology. Full article
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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