Advances in Liquid Biopsy for the Diagnosis of Breast Cancer

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Pathology and Molecular Diagnostics".

Deadline for manuscript submissions: 30 November 2024 | Viewed by 1068

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Guest Editor
Department of Surgery, Hanyang University College of Medicine, Seoul, Republic of Korea
Interests: hereditary breast cancer; male breast cancer; lymph node metastasis; tumor microenvironment
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Special Issue Information

Dear Colleagues,

Liquid biopsy refers to the process of obtaining tumor-derived material, such as tumor DNA, RNA, or extracellular vesicles, from body fluids such as blood, urine, and saliva. The relatively non-invasive nature of the material obtained using methods such as blood or urine collection makes it an attractive method of investigation compared to traditional tumor biopsy. It also has the advantage of overcoming the problem of tumor heterogeneity by sampling the entire genomic environment of the tumor present in the patient's body, and it provides the possibility of repeating the test over time, allowing for longitudinal monitoring of the tumor and anti-inflammatory drugs.

It may also have potential for the early detection of cancer, predicting prognosis, and predicting response to treatment. In recent years, the development of highly sensitive assays capable of detecting tumor-derived material in body fluids, often in minutes, has made liquid biopsy a viable alternative to conventional tumor biopsy.

Dr. Chihwan David Cha
Guest Editor

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Keywords

  • breast cancer
  • diagnosis
  • metastasis
  • ctDNA
  • liquid biopsy

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Published Papers (1 paper)

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Research

15 pages, 1746 KiB  
Article
Bone Marrow Disseminated Tumor Cell Detection Is Beneficial for the Early Finding of Bone Metastasis and Prognosis
by Yulan Wang, Jun Liu, Yanping Gong, Binjie Hu, Jianzhu Xie, Jin Cheng and Qian Huang
Diagnostics 2024, 14(15), 1629; https://doi.org/10.3390/diagnostics14151629 - 29 Jul 2024
Viewed by 771
Abstract
Background: Disseminated tumor cells (DTCs) are thought to be the initiators of tumor recurrence and metastasis. However, based on the current imaging examination methods, early detection of DTCs is extremely difficult due to their small number and dormant state. Methods: We used the [...] Read more.
Background: Disseminated tumor cells (DTCs) are thought to be the initiators of tumor recurrence and metastasis. However, based on the current imaging examination methods, early detection of DTCs is extremely difficult due to their small number and dormant state. Methods: We used the SE-iFISH approach to detect bone marrow DTCs (mDTCs) in patients with breast or prostate cancer, and compared it with various imaging examination methods to explore its role in predicting metastasis and prognosis. Results: Fifteen patients were enrolled in this study. Among them, 11 patients showed imaging-confirmed bone metastases in different sites of the body, of which seven patients had iliac mDTCs and signs of iliac bone metastases on imaging. For the remaining four patients, imaging confirmed that the bone metastatic foci were far from the ilium, but in one patient, mDTCs were detected in the ilium. Interestedly, iliac mDTCs were also detected in two out of four patients who had no sign of bone metastases on imaging. Furthermore, the epithelial marker, CK18, was ubiquitously expressed in mDTCs, but its expression was very low in peripheral circulating tumor cells (pCTCs). The Kaplan–Meier plot suggested that CK18+ mDTCs ≥ 5 was related to poor overall survival (OS) compared with that of CK18+ mDTCs < 5 in breast cancer patients (median OS: 22.1 vs. 46.9 months; log-rank, p = 0.035). Conclusions: SE-iFISH examination for mDTCs is more sensitive than the conventional methods used for detecting bone metastases. mDTC detection facilitated the early finding of tumor cells in the bone marrow and ≥5 CK18+ mDTCs was associated with a poor prognosis in breast cancer patients. Full article
(This article belongs to the Special Issue Advances in Liquid Biopsy for the Diagnosis of Breast Cancer)
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