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The Molecular Basis of Extracellular Vesicles in Health and Diseases

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (30 September 2024) | Viewed by 19896

Special Issue Editor


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Guest Editor
1. Department of Neurology, Molecular Neurogenetics Unit-West, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA
2. Department of Biomedicine, University of Bergen, Bergen, Norway
Interests: extracellular vesicles; neuro-oncology; tumor immunology; tumor microenvironment; microRNAs; gene and cell therapy
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Special Issue Information

Dear Colleagues,

Extracellular vesicles (EVs), i.e., nanoparticles with a bilayer membrane, are secreted by almost all cell types and can be found in body fluids. EVs are heterogeneous and play a crucial role in cell-to-cell communication by carrying bioactive molecules that can modulate the behavior of target cells at both close and distant sites. The composition of EVs is determined by their biogenetic molecular pathway and the microenvironment of the parent cell. Regardless of their origin, EVs carry a diverse set of bioactive molecules, including lipids, nucleic acids, and proteins, either inside the vesicle or exposed on their surface. Recent studies have provided valuable insight into the physiological and pathological roles of EVs. Technological advances in the detection, isolation, and characterization of EVs are significantly expanding and supporting research on their potential in the diagnosis and therapy of various diseases, such as cancer, cardiovascular disease, autoimmune disorders, neurological diseases, and infectious diseases.

This Special Issue aims to provide comprehensive and critical knowledge on the pre-clinical and clinical applications of EVs in relation to the pathophysiology of different diseases, evaluate their diagnostic and therapeutic potential, and review the progress in this field. The published research articles will also provide new insights into the genetic and epigenetic predictors driven by EVs involved in the etiology of multiple diseases.

Scope and information for Authors:

This Special Issue covers various areas of research on the involvement of EVs in diseases, including genetics, epigenetics, RNAs, proteomics, metabolomics, and microbiome studies. The scope ranges from the most basic to the most clinically applied, incorporating methodology, applications, and implications. We welcome submissions of research articles on the following sub-themes: extracellular vesicles and circulating nucleic acids, extracellular vesicles for the transport of genetic information and gene therapy, the transfer of nucleic acid to mediate communication by extracellular vesicles, the role of extracellular vesicles in epigenetics, microbiota-derived extracellular vesicles and their molecular cargo, extracellular vesicles as biomarkers and therapeutic targets, the heterogeneity of EVs in diseases, and new technology to isolate and characterize EVs.

The Special Issue is open access and both reviews and original research articles are welcome for submissions.

Dr. Taral R. Lunavat
Guest Editor

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Keywords

  • exosomes
  • microvesicles
  • extracellular vesicles
  • genetic material
  • therapeutics
  • diagnostics
  • biomarkers
  • molecular pathway
  • liquid biopsy
  • metabolism
  • omics

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Published Papers (12 papers)

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Research

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13 pages, 9121 KiB  
Article
PTEN Deficiency Induced by Extracellular Vesicle miRNAs from Clonorchis sinensis Potentiates Cholangiocarcinoma Development by Inhibiting Ferroptosis
by Lijia Wen, Meng Li and Jigang Yin
Int. J. Mol. Sci. 2024, 25(19), 10350; https://doi.org/10.3390/ijms251910350 - 26 Sep 2024
Viewed by 659
Abstract
The human phosphatase and tensin homolog (PTEN) is a tumor suppressor. A slight deficiency in PTEN might cause cancer susceptibility and progression. Infection by the liver fluke Clonorchis sinensis could lead to persistent loss of PTEN in cholangiocarcinoma. However, the mechanism of PTEN [...] Read more.
The human phosphatase and tensin homolog (PTEN) is a tumor suppressor. A slight deficiency in PTEN might cause cancer susceptibility and progression. Infection by the liver fluke Clonorchis sinensis could lead to persistent loss of PTEN in cholangiocarcinoma. However, the mechanism of PTEN loss and its malignant effect on cholangiocarcinoma have not yet been elucidated. Extracellular vesicles secreted by Clonorchis sinensis (CS-EVs) are rich in microRNAs (miRNAs) and can mediate communication between hosts and parasites. Herein, we delved into the miRNAs present in CS-EVs, specifically those that potentially target PTEN and modulate the progression of cholangiocarcinoma via ferroptosis mechanisms. CS-EVs were extracted by differential ultra-centrifugation for high-throughput sequencing of miRNA. Lentiviral vectors were used to construct stably transfected cell lines. Erastin was used to construct ferroptosis induction models. Finally, 36 miRNAs were identified from CS-EVs. Among them, csi-miR-96-5p inhibited PTEN expression according to the predictions and dual luciferase assay. The CCK-8 assay, xenograft tumor assays and transwell assay showed that csi-miR-96-5p overexpression and PTEN knockout significantly increased the proliferation and migration of cholangiocarcinoma cells and co-transfection of PTEN significantly reversed the effect. In the presence of erastin, the cell proliferation and migration ability of the negative transfection control group were significantly impaired, although they did not significantly change with transfection of csi-miR-96-5p and PTEN knockout, indicating that they obtained ferroptosis resistance. Mechanistically, csi-miR-96-5p and PTEN knockout significantly inhibited ferroptosis through a decrease in ferrous ion (Fe2+) and malondialdehyde (MDA), and an increase in glutathione reductase (GSH), Solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4). In conclusion, loss of PTEN promoted the progression of cholangiocarcinoma via the ferroptosis pathway and csi-miR-96-5p delivered by CS-EVs may mediate this process. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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11 pages, 1212 KiB  
Article
Clodronate Reduces ATP-Containing Microvesicle Releasing Induced by Nociceptive Stimuli in Human Keratinocytes
by Filippo Renò, Marco De Andrea, Stefano Raviola, Mario Migliario and Marco Invernizzi
Int. J. Mol. Sci. 2024, 25(15), 8435; https://doi.org/10.3390/ijms25158435 - 2 Aug 2024
Viewed by 699
Abstract
Clodronate (Clod), a first-generation bisphosphonate, acts as a natural analgesic inhibiting vesicular storage of the nociception mediator ATP by vesicular nucleotide transporter (VNUT). Epidermal keratinocytes participate in cutaneous nociception, accumulating ATP within vesicles, which are released following different stimulations. Under stress conditions, keratinocytes [...] Read more.
Clodronate (Clod), a first-generation bisphosphonate, acts as a natural analgesic inhibiting vesicular storage of the nociception mediator ATP by vesicular nucleotide transporter (VNUT). Epidermal keratinocytes participate in cutaneous nociception, accumulating ATP within vesicles, which are released following different stimulations. Under stress conditions, keratinocytes produce microvesicles (MVs) by shedding from plasma membrane evagination. MV secretion has been identified as a novel and universal mode of intercellular communication between cells. The aim of this project was to evaluate if two nociceptive stimuli, Capsaicin and Potassium Hydroxide (KOH), could stimulate MV shedding from human keratinocytes, if these MVs could contain ATP, and if Clod could inhibit this phenomenon. In our cellular model, the HaCaT keratinocyte monolayer, both Capsaicin and KOH stimulated MV release after 3 h incubation, and the released MVs contained ATP. Moreover, Clod (5 µM) was able to reduce Caps-induced MV release and abolish the one KOH induced, while the Dansylcadaverine, an endocytosis inhibitor of Clod uptake, partially failed to block the bisphosphonate activity. Based on these new data and given the role of the activation of ATP release by keratinocytes as a vehicle for nociception and pain, the “old” bisphosphonate Clodronate could provide the pharmacological basis to develop new local analgesic drugs. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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20 pages, 6068 KiB  
Article
COL6A3 Exosomes Promote Tumor Dissemination and Metastasis in Epithelial Ovarian Cancer
by Chih-Ming Ho, Ting-Lin Yen, Tzu-Hao Chang and Shih-Hung Huang
Int. J. Mol. Sci. 2024, 25(15), 8121; https://doi.org/10.3390/ijms25158121 - 25 Jul 2024
Viewed by 869
Abstract
Our study explores the role of cancer-derived extracellular exosomes (EXs), particularly focusing on collagen alpha-3 (VI; COL6A3), in facilitating tumor dissemination and metastasis in epithelial ovarian cancer (EOC). We found that COL6A3 is expressed in aggressive ES2 derivatives, SKOV3 overexpressing COL6A3 (SKOV3/COL6A3), and [...] Read more.
Our study explores the role of cancer-derived extracellular exosomes (EXs), particularly focusing on collagen alpha-3 (VI; COL6A3), in facilitating tumor dissemination and metastasis in epithelial ovarian cancer (EOC). We found that COL6A3 is expressed in aggressive ES2 derivatives, SKOV3 overexpressing COL6A3 (SKOV3/COL6A3), and mesenchymal-type ovarian carcinoma stromal progenitor cells (MSC-OCSPCs), as well as their EXs, but not in less aggressive SKOV3 cells or ES2 cells with COL6A3 knockdown (ES2/shCOL6A3). High COL6A3 expression correlates with worse overall survival among EOC patients, as evidenced by TCGA and GEO data analysis. In vitro experiments showed that EXs from MSC-OCSPCs or SKOV3/COL6A3 cells significantly enhance invasion ability in ES2 or SKOV3/COL6A3 cells, respectively (both, p <0.001). In contrast, ES2 cells with ES2/shCOL6A3 EXs exhibited reduced invasion ability (p < 0.001). In vivo, the average disseminated tumor numbers in the peritoneal cavity were significantly greater in mice receiving intraperitoneally injected SKOV3/COL6A3 cells than in SKOV3 cells (p < 0.001). Furthermore, mice intravenously (IV) injected with SKOV3/COL6A3 cells and SKOV3/COL6A3-EXs showed increased lung colonization compared to mice injected with SKOV3 cells and PBS (p = 0.007) or SKOV3/COL6A3 cells and PBS (p = 0.039). Knockdown of COL6A3 or treatment with EX inhibitor GW4869 or rapamycin-abolished COL6A3-EXs may suppress the aggressiveness of EOC. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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16 pages, 3606 KiB  
Article
Urinary Extracellular Vesicles as a Readily Available Biomarker Source: A Simplified Stratification Method
by Lidija Filipović, Milica Spasojević Savković, Radivoje Prodanović, Suzana Matijašević Joković, Sanja Stevanović, Ario de Marco, Maja Kosanović, Goran Brajušković and Milica Popović
Int. J. Mol. Sci. 2024, 25(15), 8004; https://doi.org/10.3390/ijms25158004 - 23 Jul 2024
Viewed by 1086
Abstract
Urine, a common source of biological markers in biomedical research and clinical diagnosis, has recently generated a new wave of interest. It has recently become a focus of study due to the presence of its content of extracellular vesicles (EVs). These uEVs have [...] Read more.
Urine, a common source of biological markers in biomedical research and clinical diagnosis, has recently generated a new wave of interest. It has recently become a focus of study due to the presence of its content of extracellular vesicles (EVs). These uEVs have been found to reflect physiological and pathological conditions in kidney, urothelial, and prostate tissue and can illustrate further molecular processes, leading to a rapid expansion of research in this field In this work, we present the advantages of an immunoaffinity-based method for uEVs’ isolation with respect to the gold standard purification approach performed by differential ultracentrifugation [in terms of purity and antigen presence. The immunoaffinity method was made feasible by combining specific antibodies with a functionalized polymethacrylate polymer. Flow cytometry indicated a significant fluorescence shift, validating the presence of the markers (CD9, CD63, CD81) and confirming the effectiveness of the isolation method. Microscopy evaluations have shown that the morphology of the vesicles remained intact and corresponded to the expected shapes and dimensions of uEVs. The described protocol is inexpensive, fast, easy to process, has good reproducibility, and can be applied to further biological samples. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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18 pages, 3463 KiB  
Article
Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice
by Béatrice Schaack, Corinne Mercier, Maya Katby, Dalil Hannani, Julien Vollaire, Julie Suzanne Robert, Clément Caffaratti, Françoise Blanquet, Olivier Nicoud, Véronique Josserand and David Laurin
Int. J. Mol. Sci. 2024, 25(3), 1821; https://doi.org/10.3390/ijms25031821 - 2 Feb 2024
Cited by 5 | Viewed by 1830
Abstract
A cell’s ability to secrete extracellular vesicles (EVs) for communication is present in all three domains of life. Notably, Gram-negative bacteria produce a specific type of EVs called outer membrane vesicles (OMVs). We previously observed the presence of OMVs in human blood, which [...] Read more.
A cell’s ability to secrete extracellular vesicles (EVs) for communication is present in all three domains of life. Notably, Gram-negative bacteria produce a specific type of EVs called outer membrane vesicles (OMVs). We previously observed the presence of OMVs in human blood, which could represent a means of communication from the microbiota to the host. Here, in order to investigate the possible translocation of OMVs from the intestine to other organs, the mouse was used as an animal model after OMVs administration. To achieve this, we first optimized the signal of OMVs containing the fluorescent protein miRFP713 associated with the outer membrane anchoring peptide OmpA by adding biliverdin, a fluorescence cofactor, to the cultures. The miRFP713-expressing OMVs produced in E. coli REL606 strain were then characterized according to their diameter and protein composition. Native- and miRFP713-expressing OMVs were found to produce homogenous populations of vesicles. Finally, in vivo and ex vivo fluorescence imaging was used to monitor the distribution of miRFP713-OMVs in mice in various organs whether by intravenous injection or oral gavage. The relative stability of the fluorescence signals up to 3 days post-injection/gavage paves the way to future studies investigating the OMV-based communication established between the different microbiotas and their host. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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12 pages, 4544 KiB  
Article
Human Umbilical Cord Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Reduce Skin Inflammation In Vitro
by Tzou-Yien Lin, Tsong-Min Chang, Wei-Cheng Tsai, Yi-Ju Hsieh, Li-Ting Wang and Huey-Chun Huang
Int. J. Mol. Sci. 2023, 24(23), 17109; https://doi.org/10.3390/ijms242317109 - 4 Dec 2023
Cited by 1 | Viewed by 2196
Abstract
The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h before oxazolone exposure. The [...] Read more.
The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h before oxazolone exposure. The pretreated UVMSC-EVs showed protective activity, elevating cell viability, reducing intracellular ROS, and reducing the changes in the mitochondrial membrane potential compared to the cells with a direct oxazolone treatment alone. The UCMSC-EVs exhibited anti-inflammatory activity via reducing the inflammatory cytokines IL-1β and TNF-α. A mechanism study showed that the UCMSC-EVs increased the protein expression levels of SIRT1 and P53 and reduced P65 protein expression. It was concluded that UVMSC-EVs can induce the antioxidant defense systems of HaCaT cells and that they may have potential as functional ingredients in anti-aging cosmetics for skin care. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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16 pages, 2683 KiB  
Article
RNA Profiles of Tear Fluid Extracellular Vesicles in Patients with Dry Eye-Related Symptoms
by Tanya Cross, Reidun Øvstebø, Berit Sletbakk Brusletto, Anne-Marie Siebke Trøseid, Ole Kristoffer Olstad, Trude Aspelin, Catherine Joan Jackson, Xiangjun Chen, Tor Paaske Utheim and Kari Bente Foss Haug
Int. J. Mol. Sci. 2023, 24(20), 15390; https://doi.org/10.3390/ijms242015390 - 20 Oct 2023
Cited by 4 | Viewed by 2164
Abstract
Currently, diagnosing and stratifying dry eye disease (DED) require multiple tests, motivating interest in a single definitive test. The purpose of this study was to investigate the potential for using tear fluid extracellular vesicle (EV)-RNA in DED diagnostics. With a role in intercellular [...] Read more.
Currently, diagnosing and stratifying dry eye disease (DED) require multiple tests, motivating interest in a single definitive test. The purpose of this study was to investigate the potential for using tear fluid extracellular vesicle (EV)-RNA in DED diagnostics. With a role in intercellular communication, nanosized EVs facilitate the protected transport of diverse bioactive molecules in biofluids, including tears. Schirmer strips were used to collect tears from 10 patients presenting with dry eye-related symptoms at the Norwegian Dry Eye Clinic. The samples comprised two groups, five from patients with a tear film break-up time (TBUT) of 2 s and five from patients with a TBUT of 10 s. Tear fluid EV-RNA was isolated using a Qiagen exoRNeasy Midi Kit, and the RNA was characterized using Affymetrix ClariomTM D microarrays. The mean signal values of the two groups were compared using a one-way ANOVA. A total of 26,639 different RNA transcripts were identified, comprising both mRNA and ncRNA subtypes. Approximately 6% of transcripts showed statistically significant differential abundance between the two groups. The mRNA sodium channel modifier 1 (SCNM1) was detected at a level 3.8 times lower, and the immature microRNA-130b was detected at a level 1.5 times higher in the group with TBUT 2 s compared to the group with TBUT 10 s. This study demonstrates the potential for using tear fluid EV-RNA in DED diagnostics. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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22 pages, 4670 KiB  
Article
Exosomes Derived from Hypoxia-Cultured Human Adipose Stem Cells Alleviate Articular Chondrocyte Inflammaging and Post-Traumatic Osteoarthritis Progression
by Ling-Hua Chang, Shun-Cheng Wu, Chung-Hwan Chen, Jhen-Wei Chen, Wan-Chun Huang, Che-Wei Wu, Yi-Shan Lin, Yu-Ju Chen, Je-Ken Chang and Mei-Ling Ho
Int. J. Mol. Sci. 2023, 24(17), 13414; https://doi.org/10.3390/ijms241713414 - 29 Aug 2023
Cited by 12 | Viewed by 2877
Abstract
Osteoarthritis (OA) is the most common age-related degenerative joint disease. Inflammaging, linking inflammation and aging, is found in senescent cells with the secretions of matrix-degrading proteins and proinflammatory cytokines. The senescence-associated secretory phenotype (SASP) plays a very important role in OA progression. However, [...] Read more.
Osteoarthritis (OA) is the most common age-related degenerative joint disease. Inflammaging, linking inflammation and aging, is found in senescent cells with the secretions of matrix-degrading proteins and proinflammatory cytokines. The senescence-associated secretory phenotype (SASP) plays a very important role in OA progression. However, there remains no effective way to suppress OA progression, especially by suppressing inflammaging and/or the chondrocyte SASP. Recent studies have shown that exosomes derived from hypoxia-cultured BMSCs can regenerate cartilage in OA animal models. Some reports have further indicated that exosomes secreted from MSCs contribute to the efficacy of MSC therapy in OA. However, whether hypoxia-cultured ADSC-secreted exosomes (hypoxia-ADSC-Exos) can alleviate the chondrocyte SASP or OA progression remains unclear. Accordingly, we hypothesized that hypoxia-ADSC-Exos have a beneficial effect on the normal functions of human articular chondrocytes (HACs), can attenuate the SASP of OA-like HACs in vitro, and further suppress OA progression in rats. Hypoxia-ADSC-Exos were derived from ADSCs cultured in 1% O2 and 10% de-Exo-FBS for 48 h. The molecular and cell biological effects of hypoxia-ADSC-Exos were tested on IL1-β-induced HACs as OA-like HACs in vitro, and the efficacy of OA treatment was tested in ACLT-induced OA rats. The results showed that hypoxia-ADSC-Exos had the best effect on GAG formation in normal HACs rather than those cultured in normoxia or hypoxia plus 2% de-Exo-FBS. We further found that hypoxia-ADSC-Exos alleviated the harmful effect in OA-like HACs by decreasing markers of normal cartilage (GAG and type II collagen) and increasing markers of fibrous or degenerative cartilage (type I or X collagen), matrix degradation enzymes (MMP13 and ADAMT5), and inflammatory cytokines (TNFα and IL-6). More importantly, intra-articular treatment with hypoxia-ADSC-Exos suppressed OA progression, as evidenced by the weight-bearing function test and cartilage GAG quantification in ACLT rats. Moreover, through NGS and bioinformatic analysis, seven potential miRNAs were found in hypoxia-ADSC-Exos, which may contribute to regulating cellular oxidative stress and attenuating cell senescence. In summary, we demonstrated that hypoxia-ADSC-Exos, carrying potent miRNAs, not only improve normal HAC function but also alleviate HAC inflammaging and OA progression. The results suggest that hypoxia-ADSC-Exo treatment may offer another strategy for future OA therapy. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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Review

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15 pages, 1309 KiB  
Review
Progress of Exosomal LncRNAs in Pancreatic Cancer
by Chengyan Wei, Chunwei Zhang, Yuanzhi Zhou, Jingjing Wang and Yong Jin
Int. J. Mol. Sci. 2024, 25(16), 8665; https://doi.org/10.3390/ijms25168665 - 8 Aug 2024
Viewed by 888
Abstract
Pancreatic cancer is a prevalent malignant tumor with rising medication resistance and mortality. Due to a dearth of specific and trustworthy biomarkers and therapeutic targets, pancreatic cancer early detection and treatment are still not at their best. Exosomal LncRNAs have been found to [...] Read more.
Pancreatic cancer is a prevalent malignant tumor with rising medication resistance and mortality. Due to a dearth of specific and trustworthy biomarkers and therapeutic targets, pancreatic cancer early detection and treatment are still not at their best. Exosomal LncRNAs have been found to be plentiful and persistent within exosomes, and they are capable of functioning whether the exosomes are traveling to close or distant cells. Furthermore, increasing evidence suggests that exosomal LncRNA, identified as an oncogene or tumor suppressor-control the growth, metastasis, and susceptibility of pancreatic cancer to chemotherapy and radiation therapy. Promising prospects for both antitumor targets and diagnostic biomarkers are exosomal LncRNAs. The primary features of exosomal LncRNAs, their biological roles in the onset and progression of pancreatic cancer, and their potential as therapeutic targets and diagnostic molecular markers are outlined in this review. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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24 pages, 2089 KiB  
Review
Emerging Roles of Exosomes in Stroke Therapy
by Anthony Larson, Dilmareth E. Natera-Rodriguez, Andrew Crane, Dana Larocca, Walter C. Low, Andrew W. Grande and Jieun Lee
Int. J. Mol. Sci. 2024, 25(12), 6507; https://doi.org/10.3390/ijms25126507 - 13 Jun 2024
Viewed by 1466
Abstract
Stroke is the number one cause of morbidity in the United States and number two cause of death worldwide. There is a critical unmet medical need for more effective treatments of ischemic stroke, and this need is increasing with the shift in demographics [...] Read more.
Stroke is the number one cause of morbidity in the United States and number two cause of death worldwide. There is a critical unmet medical need for more effective treatments of ischemic stroke, and this need is increasing with the shift in demographics to an older population. Recently, several studies have reported the therapeutic potential of stem cell-derived exosomes as new candidates for cell-free treatment in stoke. This review focuses on the use of stem cell-derived exosomes as a potential treatment tool for stroke patients. Therapy using exosomes can have a clear clinical advantage over stem cell transplantation in terms of safety, cost, and convenience, as well as reducing bench-to-bed latency due to fewer regulatory milestones. In this review article, we focus on (1) the therapeutic potential of exosomes in stroke treatment, (2) the optimization process of upstream and downstream production, and (3) preclinical application in a stroke animal model. Finally, we discuss the limitations and challenges faced by exosome therapy in future clinical applications. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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20 pages, 995 KiB  
Review
The Role of Extracellular Vesicles in Allergic Sensitization: A Systematic Review
by Davis Tucis, Georgina Hopkins, William Browne, Victoria James, David Onion and Lucy C. Fairclough
Int. J. Mol. Sci. 2024, 25(8), 4492; https://doi.org/10.3390/ijms25084492 - 19 Apr 2024
Viewed by 1124
Abstract
Allergies affect approximately 10–30% of people worldwide, with an increasing number of cases each year; however, the underlying mechanisms are still poorly understood. In recent years, extracellular vesicles (EVs) have been suggested to play a role in allergic sensitization and skew to a [...] Read more.
Allergies affect approximately 10–30% of people worldwide, with an increasing number of cases each year; however, the underlying mechanisms are still poorly understood. In recent years, extracellular vesicles (EVs) have been suggested to play a role in allergic sensitization and skew to a T helper type 2 (Th2) response. The aim of this review is to highlight the existing evidence of EV involvement in allergies. A total of 22 studies were reviewed; 12 studies showed EVs can influence a Th2 response, while 10 studies found EVs promoted a Th1 or Treg response. EVs can drive allergic sensitization through up-regulation of pro-Th2 cytokines, such as IL-4 and IL-13. In addition, EVs from MRSA can induce IgE hypersensitivity in mice towards MRSA. On the other hand, EVs can induce tolerance in the immune system; for example, pre-exposing OVA-loaded EVs prevented OVA sensitization in mice. The current literature thus suggests that EVs play an essential role in allergy. Further research utilizing human in vitro models and clinical studies is needed to give a reliable account of the role of EVs in allergy. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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25 pages, 1831 KiB  
Review
The Regulation of Exosome Generation and Function in Physiological and Pathological Processes
by Ying Wang, Tong Xiao, Chaoran Zhao and Guiying Li
Int. J. Mol. Sci. 2024, 25(1), 255; https://doi.org/10.3390/ijms25010255 - 23 Dec 2023
Cited by 8 | Viewed by 2516
Abstract
Exosomes, a type of extracellular vesicle with a diameter of approximately 100 nm that is secreted by all cells, regulate the phenotype and function of recipient cells by carrying molecules such as proteins, nucleic acids, and lipids and are important mediators of intercellular [...] Read more.
Exosomes, a type of extracellular vesicle with a diameter of approximately 100 nm that is secreted by all cells, regulate the phenotype and function of recipient cells by carrying molecules such as proteins, nucleic acids, and lipids and are important mediators of intercellular communication. Exosomes are involved in various physiological and pathological processes such as immunomodulation, angiogenesis, tumorigenesis, metastasis, and chemoresistance. Due to their excellent properties, exosomes have shown their potential application in the clinical diagnosis and treatment of disease. The functions of exosomes depend on their biogenesis, uptake, and composition. Thus, a deeper understanding of these processes and regulatory mechanisms can help to find new targets for disease diagnosis and therapy. Therefore, this review summarizes and integrates the recent advances in the regulatory mechanisms of the entire biological process of exosomes, starting from the formation of early-sorting endosomes (ESCs) by plasma membrane invagination to the release of exosomes by fusion of multivesicular bodies (MVBs) with the plasma membrane, as well as the regulatory process of the interactions between exosomes and recipient cells. We also describe and discuss the regulatory mechanisms of exosome production in tumor cells and the potential of exosomes used in cancer diagnosis and therapy. Full article
(This article belongs to the Special Issue The Molecular Basis of Extracellular Vesicles in Health and Diseases)
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