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Cutting-Edge Insights into Oral Health and Disease

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (30 July 2024) | Viewed by 1907

Special Issue Editor


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Guest Editor
Department of Human Sciences, Innovation and Territory, School of Dental Hygiene, Postgraduate School of Orthodontics, University of Insubria, 22100 Como, Italy
Interests: oral health prevention; orthodontics;dentistry
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Special Issue Information

Dear Colleague,

Oral microbial communities are now seen as the fundamental etiological agent in oral diseases through their interface with host inflammatory responses. Oral infections occur frequently and often lead to chronic inflammations affecting the teeth (i.e., caries), the gingival tissues surrounding the teeth (i.e., gingivitis and endodontic lesions), and the tooth-supporting structures (i.e., periodontitis). Research in biology and microbiology is essential to promote improvements in molecule diagnosis and therapies in oral diseases.

Furthermore, it is critical to re-evaluate the molecule mechanisms behind the development and maintenance of oral health and to analyse the findings of studies on the anatomical, functional, and molecular changes in masticatory function. In order to sustain a long-term, healthy population, it is necessary to have a deeper knowledge of the biological and chemical processes of the muscles, salivary glands, and maxillofacial structure. This Special Issue has the aim of promoting research on the pathophysiology and microbiology of oral diseases, in particular masticatory muscle physiology, periodontal disease, and oral inflammatory diseases.

Prof. Dr. Luca Levrini
Guest Editor

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Keywords

  • oral diseases
  • masticatory muscle
  • periodontal disease
  • inflammatory responses

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Published Papers (1 paper)

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Research

20 pages, 28331 KiB  
Article
Revealing Genetic Dynamics: scRNA-seq Unravels Modifications in Human PDL Cells across In Vivo and In Vitro Environments
by Ali T. Abdallah, Michael Peitz and Anna Konermann
Int. J. Mol. Sci. 2024, 25(9), 4731; https://doi.org/10.3390/ijms25094731 - 26 Apr 2024
Viewed by 1072
Abstract
The periodontal ligament (PDL) is a highly specialized fibrous tissue comprising heterogeneous cell populations of an intricate nature. These complexities, along with challenges due to cell culture, impede a comprehensive understanding of periodontal pathophysiology. This study aims to address this gap, employing single-cell [...] Read more.
The periodontal ligament (PDL) is a highly specialized fibrous tissue comprising heterogeneous cell populations of an intricate nature. These complexities, along with challenges due to cell culture, impede a comprehensive understanding of periodontal pathophysiology. This study aims to address this gap, employing single-cell RNA sequencing (scRNA-seq) technology to analyze the genetic intricacies of PDL both in vivo and in vitro. Primary human PDL samples (n = 7) were split for direct in vivo analysis and cell culture under serum-containing and serum-free conditions. Cell hashing and sorting, scRNA-seq library preparation using the 10x Genomics protocol, and Illumina sequencing were conducted. Primary analysis was performed using Cellranger, with downstream analysis via the R packages Seurat and SCORPIUS. Seven distinct PDL cell clusters were identified comprising different cellular subsets, each characterized by unique genetic profiles, with some showing donor-specific patterns in representation and distribution. Formation of these cellular clusters was influenced by culture conditions, particularly serum presence. Furthermore, certain cell populations were found to be inherent to the PDL tissue, while others exhibited variability across donors. This study elucidates specific genes and cell clusters within the PDL, revealing both inherent and context-driven subpopulations. The impact of culture conditions—notably the presence of serum—on cell cluster formation highlights the critical need for refining culture protocols, as comprehending these influences can drive the creation of superior culture systems vital for advancing research in PDL biology and regenerative therapies. These discoveries not only deepen our comprehension of PDL biology but also open avenues for future investigations into uncovering underlying mechanisms. Full article
(This article belongs to the Special Issue Cutting-Edge Insights into Oral Health and Disease)
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