ijms-logo

Journal Browser

Journal Browser

Updates & New Concepts in Collagen 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 2022

Special Issue Editor


E-Mail Website
Guest Editor
Nippi Research Institute of Biomatrix, 520-11 Kuwabara, Toride 302-0017, Japan
Interests: collagen; cells
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, environmental issues have been attracting considerable attention on a global scale. In the microscopic world of living organisms, it is the extracellular matrix that provides an environment for cellular activities. Among the many extracellular matrix proteins, collagen is the most abundant, and various types of collagen support organs and tissues of the body (such as fibers and basement membranes). In addition, collagen has been shown to function as a cell adhesion protein and a reservoir for various liquid factors. Collagen is expected to be applied to 3D culture substrates for organoids and regenerative medicine. Furthermore, it is becoming clear that collagen peptides, which are degraded products of collagen, are taken into the body and work to control cell functions. In this Special Issue, we would like to showcase review articles and recent original papers that serve as a platform to help gain a comprehensive understanding of the diversity of collagen—a macromolecule with a supramolecular structure and microscopic functions—and to explore new ways of utilizing collagen.

Dr. Shunji Hattori
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • collagen
  • regenerative medicine
  • cell adhesion
  • collagen peptide

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

17 pages, 29339 KiB  
Article
Optimization of Sirius Red-Based Microplate Assay to Investigate Collagen Production In Vitro
by Csenge Szász, Domonkos Pap, Beáta Szebeni, Péter Bokrossy, László Őrfi, Attila J. Szabó, Ádám Vannay and Apor Veres-Székely
Int. J. Mol. Sci. 2023, 24(24), 17435; https://doi.org/10.3390/ijms242417435 - 13 Dec 2023
Cited by 1 | Viewed by 1678
Abstract
Tissue fibrosis is characterized by chronic fibroblast activation and consequently excessive accumulation of collagen-rich extracellular matrix. In vitro microplate-based assays are essential to investigate the underlying mechanism and the effect of antifibrotic drugs. In this study, in the absence of a gold-standard method, [...] Read more.
Tissue fibrosis is characterized by chronic fibroblast activation and consequently excessive accumulation of collagen-rich extracellular matrix. In vitro microplate-based assays are essential to investigate the underlying mechanism and the effect of antifibrotic drugs. In this study, in the absence of a gold-standard method, we optimized a simple, cost-effective, Sirius Red-based colorimetric measurement to determine the collagen production of fibroblasts grown on 96-well tissue culture plates. Based on our findings, the use of a serum-free medium is recommended to avoid aspecific signals, while ascorbate supplementation increases the collagen production of fibroblasts. The cell-associated collagens can be quantified by Sirius Red staining in acidic conditions followed by alkaline elution. Immature collagens can be precipitated from the culture medium by acidic Sirius Red solution, and after subsequent centrifugation and washing steps, their amount can be also measured. Increased attention has been paid to optimizing the assay procedure, including incubation time, temperature, and solution concentrations. The resulting assay shows high linearity and sensitivity and could serve as a useful tool in fibrosis-related basic research as well as in preclinical drug screening. Full article
(This article belongs to the Special Issue Updates & New Concepts in Collagen 2.0)
Show Figures

Figure 1

Back to TopTop