International Journal of Molecular Sciences

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Dear Colleagues,

Bone remodeling is a tightly regulated process involving the interplay among osteoclasts, osteoblasts, and osteocytes. Osteoclasts are the bone resorbing cells arising from the fusion of monocyte/macrophage precursors. Osteoclastogenesis is primarily supported by osteoblasts but also by other cells of the bone marrow microenvironment, such as immune cells, adipocytes, and endothelial cells. Osteoclastogenesis is a finely controlled process, and its deregulation is related to different bone diseases; thus, the deepening of the mechanisms regulating osteoclast formation can be useful for identifying new pharmacological targets.

This special issue encourages submissions of full-review or original research papers, and perspectives on the biological, functional, and mechanistic question(s) being addressed.

Dr. Giacomina Brunetti
Guest Editor

Manuscript Submission Information

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Related Special Issue

Published Papers (1 paper)

Research

12 pages, 1698 KiB

Open AccessArticle

Porphyromonas gingivalis Fimbriae Induce Osteoclastogenesis via Toll-like Receptors in RAW264 Cells

by Yuki Suzuki, Takeshi Kikuchi, Hisashi Goto, Yuhei Takayanagi, Shotaro Kawamura, Noritaka Sawada, Yoshikazu Naiki, Hisataka Kondo, Jun-ichiro Hayashi, Yoshiaki Hasegawa and Akio Mitani

Int. J. Mol. Sci. 2022, 23(23), 15293; https://doi.org/10.3390/ijms232315293 - 4 Dec 2022

Cited by 6 | Viewed by 1467

Abstract

The effect of Mfa1 fimbriae of Porphyromonas gingivalis on the progression of bone resorption remains unclear, especially compared with another fimbriae, FimA. We investigated the effect of Mfa1 on osteoclastogenesis together with FimA. We also investigated the role of Toll-like receptors (TLRs) in Mfa1 recognition during osteoclast differentiation. Receptor activator of nuclear factor κβ ligand (RANKL)-prestimulated RAW264 cells were used to examine the effects of purified Mfa1 fimbriae. The number of osteoclasts was examined by tartrate-resistant acid phosphate (TRAP) staining, osteoclast activation was investigated by bone resorption assays, and gene expression of differentiation markers was examined by quantitative real-time PCR. Transfection of Tlr2 and Tlr4 siRNAs into RAW264 cells was also employed and their role in Mfa1 recognition was investigated. Mfa1 effectively induced the formation of TRAP-positive multinucleated cells and activated osteoclasts. Mfa1 also increased gene expression of Acp5, Mmp9, and Ctsk in RANKL-prestimulated RAW264 cells compared with the control. The osteoclastogenesis induced by Mfa1 was significantly decreased in cells transfected with Tlr2 or Tlr4 siRNAs compared with control siRNA. Our results revealed the role of Mfa1 fimbriae in osteoclastogenesis that may contribute to the partial elucidation of the mechanisms of periodontal disease progression and the development of new therapeutic strategies. Full article

(This article belongs to the Special Issue 2nd Edition: Molecular Mechanisms Regulating Osteoclastogenesis)

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