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11 pages, 862 KiB

Open AccessArticle

Plant Abandonment by Busseola fusca (Lepidoptera: Noctuidae) Larvae: Do Bt Toxins Have an Effect?

by Andri Visser, Hannalene Du Plessis, Annemie Erasmus and Johnnie Van den Berg

Insects 2020, 11(2), 77; https://doi.org/10.3390/insects11020077 - 22 Jan 2020

Cited by 3 | Viewed by 2711

Abstract

Busseola fusca (Fuller; Lepidoptera: Noctuidae) is an important pest of maize in Africa and can be effectively controlled by Bt maize. However, the sustainability of this technology is threatened by resistance evolution, which necessitates the implementation of the high-dose/refuge insect resistance management (IRM) [...] Read more.

Busseola fusca (Fuller; Lepidoptera: Noctuidae) is an important pest of maize in Africa and can be effectively controlled by Bt maize. However, the sustainability of this technology is threatened by resistance evolution, which necessitates the implementation of the high-dose/refuge insect resistance management (IRM) strategy. Despite the success of this IRM strategy, it is based on several assumptions about insect-hostplant interactions that are not always valid for different pest species. In this study, the plant abandonment behavior of Cry1Ab-resistant and susceptible B. fusca larvae were evaluated on a non-Bt, single toxin (Cry1Ab), and a pyramid event (Cry1.105 + Cry2Ab2) of maize over a four-day period. The aim was to determine if larvae are more likely to abandon maize plants that contain Bt-toxins than conventional non-Bt plants, and if resistance to the Cry1Ab-toxin affects this behavior. This study found that both Bt-resistant and susceptible B. fusca neonate larvae show feeding avoidance behavior and increased plant abandonment rates when exposed to Bt maize leaf tissue. The implications of these findings for the design of IRM strategies and choice of refuge structures are discussed in the context of Bt maize in Africa. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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24 pages, 4048 KiB

Open AccessArticle

Larval Migration Behaviour of Busseola fusca (Lepidoptera: Noctuidae) on Bt and Non-Bt Maize under Semi-Field and Field Conditions

by Andri Visser, Hannalene Du Plessis, Annemie Erasmus and Johnnie van den Berg

Insects 2020, 11(1), 16; https://doi.org/10.3390/insects11010016 - 23 Dec 2019

Cited by 2 | Viewed by 2750

Abstract

Busseola fusca (Fuller) (Lepidoptera: Noctuidae) is a destructive pest of maize throughout the African continent. Bt maize is an effective control measure for this pest, however, selection pressure for resistance evolution is high. This necessitates the implementation of insect resistance management (IRM) strategies [...] Read more.

Busseola fusca (Fuller) (Lepidoptera: Noctuidae) is a destructive pest of maize throughout the African continent. Bt maize is an effective control measure for this pest, however, selection pressure for resistance evolution is high. This necessitates the implementation of insect resistance management (IRM) strategies such as the high-dose/refuge strategy. This IRM strategy relies on the validity of several assumptions about the behaviour of pests during insect-hostplant interactions. In this study, the migration behaviour of B. fusca larvae was evaluated in a semi-field (greenhouse) and field setting. The effect of factors such as different Cry proteins, plant growth stage at infestation, and plant density on the rate and distance of larval migration were investigated over four and five week periods. Migration of the larvae were recorded by using both a leaf feeding damage rating scale and destructive sampling at the end of the trials. Results indicated that B. fusca larval migration success was significantly affected by plant growth stage and plant density—while limited larval migration was recorded in plots inoculated with larvae at a late growth stage (V10), higher plant density facilitated increased interplant migration. The results also suggest that B. fusca larvae do not migrate extensively (rarely further than two plants from the natal plant) and that larval mortality is high. Implications for IRM strategies are discussed. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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10 pages, 1456 KiB

Open AccessArticle

Cold Tolerance and Population Dynamics of Leptoglossus zonatus (Hemiptera: Coreidae)

by Kristen E. Tollerup

Insects 2019, 10(10), 351; https://doi.org/10.3390/insects10100351 - 17 Oct 2019

Cited by 4 | Viewed by 2391

Abstract

In California’s San Joaquin Valley, feeding by the coreid pest, Leptoglossus zonatus, can cause considerable economic loss on almond and pistachio. This research was conducted to improve understanding of how winter temperatures affect mortality of overwintering adult L. zonatus and to develop [...] Read more.

In California’s San Joaquin Valley, feeding by the coreid pest, Leptoglossus zonatus, can cause considerable economic loss on almond and pistachio. This research was conducted to improve understanding of how winter temperatures affect mortality of overwintering adult L. zonatus and to develop a better understanding of the role pomegranate plays in the species’ life-history. We exposed 7410 field-collected adult L. zonatus to temperatures between −2 and −10 °C for a period of three, four, or six hours using insect incubators. At six hours of exposure, the, LD₅₀ and LD₉₅ occur at −5.8 and −9.7 °C, respectively. We classified L. zonatus as chill-intolerant. Temperatures cold enough to affect substantial mortality of overwintering L. zonatus rarely occur in the San Joaquin Valley. Whole aggregation destructive sampling from a pomegranate hedgerow in Fresno County was conducted to determine population dynamics. At late summer to early fall, aggregations consisted of >90% immature stages. By early to mid-winter, mean aggregation size decreased, consisting of only three to 12 late-instars and adults. During years one and two of the experiment, L. zonatus produced a generation on pomegranate, mostly between September and mid-November. Overwintering did not occur on pomegranate, rather the majority of adults emigrated to other overwintering locations by mid-winter. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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13 pages, 3636 KiB

Open AccessArticle

Codling Moth Wing Morphology Changes Due to Insecticide Resistance

by Ivana Pajač Živković, Hugo Alejandro Benitez, Božena Barić, Zrinka Drmić, Martina Kadoić Balaško, Darija Lemic, Jose H. Dominguez Davila, Katarina Maryann Mikac and Renata Bažok

Insects 2019, 10(10), 310; https://doi.org/10.3390/insects10100310 - 21 Sep 2019

Cited by 9 | Viewed by 3711

Abstract

The codling moth (CM) (Cydia pomonella L.) is the most important apple pest in Croatia and Europe. Owing to its economic importance, it is a highly controlled species and the intense selection pressure the species is under has likely caused it to [...] Read more.

The codling moth (CM) (Cydia pomonella L.) is the most important apple pest in Croatia and Europe. Owing to its economic importance, it is a highly controlled species and the intense selection pressure the species is under has likely caused it to change its phenotype in response. Intensive application of chemical-based insecticide treatments for the control of CM has led to resistance development. In this study, the forewing morphologies of 294 CM (11 populations) were investigated using geometric morphometric procedures based on the venation patterns of 18 landmarks. Finite element method (FEM) was also used to further investigate the dispersal capabilities of moths by modelling wing deformation versus wind speed. Three treatments were investigated and comprised populations from integrated and ecological (susceptible) orchards and laboratory-reared non-resistant populations. Forewing shape differences were found among the three treatment populations investigated. Across all three population treatments, the movement of landmarks 1, 7, 8, 9, and 12 drove the wing shape differences found. A reliable pattern of differences in forewing shape as related to control practice type was observed. FEM revealed that as wind speed (m/s⁻¹) increased, so too did wing deformation (mm) for CM from each of the three treatments modelled. CM from the ecological orchards displayed the least deformation followed by integrated then laboratory-reared CM, which had the highest wing deformation at the highest wind speeds. This study presents an affordable and accessible technique that reliably demonstrates wing shape differences, and thus its use as a population biomarker to detect resistance should be further investigated. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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18 pages, 4827 KiB

Open AccessArticle

Tolerance of High Oral Doses of Nonradioactive and Radioactive Caesium Chloride in the Pale Grass Blue Butterfly Zizeeria maha

by Raj D. Gurung, Wataru Taira, Ko Sakauchi, Masaki Iwata, Atsuki Hiyama and Joji M. Otaki

Insects 2019, 10(9), 290; https://doi.org/10.3390/insects10090290 - 9 Sep 2019

Cited by 11 | Viewed by 3448

Abstract

The biological effects of the Fukushima nuclear accident have been examined in the pale grass blue butterfly, Zizeeria maha (Lepidoptera: Lycaenidae). In previous internal exposure experiments, larvae were given field-collected contaminated host plant leaves that contained up to 43.5 kBq/kg (leaf) of radioactive [...] Read more.

The biological effects of the Fukushima nuclear accident have been examined in the pale grass blue butterfly, Zizeeria maha (Lepidoptera: Lycaenidae). In previous internal exposure experiments, larvae were given field-collected contaminated host plant leaves that contained up to 43.5 kBq/kg (leaf) of radioactive caesium. Larvae ingested up to 480 kBq/kg (larva), resulting in high mortality and abnormality rates. However, these results need to be compared with the toxicological data of caesium. Here, we examined the toxicity of both nonradioactive and radioactive caesium chloride on the pale grass blue butterfly. Larvae were fed a caesium-containing artificial diet, ingesting up to 149 MBq/kg (larva) of radioactive caesium (¹³⁷Cs) or a much higher amount of nonradioactive caesium. We examined the pupation rate, eclosion rate, survival rate up to the adult stage, and the forewing size. In contrast to previous internal exposure experiments using field-collected contaminated leaves, we could not detect any effect. We conclude that the butterfly is tolerant to ionising radiation from ¹³⁷Cs in the range tested but is vulnerable to radioactive contamination in the field. These results suggest that the biological effects in the field may be mediated through ecological systems and cannot be estimated solely based on radiation doses. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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16 pages, 639 KiB

Open AccessArticle

Investigation of Relative Development and Reproductivity Fitness Cost in Three Insecticide-Resistant Strains of Aedes aegypti from Thailand

by Jassada Saingamsook, Jintana Yanola, Nongkran Lumjuan, Catherine Walton and Pradya Somboon

Insects 2019, 10(9), 265; https://doi.org/10.3390/insects10090265 - 22 Aug 2019

Cited by 25 | Viewed by 3688

Abstract

Knockdown resistance (kdr) and detoxification enzymes are major resistance mechanisms in insecticide-resistant Aedes aegypti throughout the world. Persistence of the resistance phenotype is associated with high fitness of resistance alleles in the absence of insecticide pressure. This study determined the relative [...] Read more.

Knockdown resistance (kdr) and detoxification enzymes are major resistance mechanisms in insecticide-resistant Aedes aegypti throughout the world. Persistence of the resistance phenotype is associated with high fitness of resistance alleles in the absence of insecticide pressure. This study determined the relative fitness cost of three insecticide-resistant strains of Aedes aegypti—PMD, PMD-R, and UPK-R—and a hybrid under similar laboratory conditions in the absence of insecticide. The PMD strain is resistant to DDT with no kdr alleles; the PMD-R is resistant to DDT and permethrin with 1534C homozygous kdr alleles; and UPK-R is resistant to DDT, permethrin, and deltamethrin with 989P + 1016G homozygous alleles. The DDT-resistant PMD strain had the highest fitness compared with the two DDT/pyrethroid-resistant strains (PMD-R and UPK-R) and hybrid. Consistent fitness costs were observed in the DDT/pyrethroid-resistant strains and hybrid, including shorter wing length, reduced egg hatchability, shorter female lifespan, and shorter viability of eggs after storage, whereas no effect was observed on blood feeding rate. In addition, reduced egg production was observed in the PMD-R strain and prolonged developmental time was seen in the UPK-R strain. The corresponding hybrid that is heterozygous for kdr alleles was fitter than either of the homozygous mutant genotypes. This is in accordance with the high frequency of heterozygous genotypes observed in natural populations of Ae. aegypti in Chiang Mai city. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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10 pages, 1119 KiB

Open AccessArticle

A Re-Evaluation of Olive Fruit Fly Organophosphate-Resistant Ace Alleles in Iberia, and Field-Testing Population Effects after in-Practice Dimethoate Use

by Tânia Nobre, Luis Gomes and Fernando Trindade Rei

Insects 2019, 10(8), 232; https://doi.org/10.3390/insects10080232 - 1 Aug 2019

Cited by 5 | Viewed by 3337

Abstract

The management of the olive fruit fly (Bactrocera oleae) is traditionally based upon the use of organophosphate insecticides, mainly dimethoate. In this evolutionary arms race between man and pest, the flies have adapted a pesticide resistance, implying two point-mutations of the [...] Read more.

The management of the olive fruit fly (Bactrocera oleae) is traditionally based upon the use of organophosphate insecticides, mainly dimethoate. In this evolutionary arms race between man and pest, the flies have adapted a pesticide resistance, implying two point-mutations of the Ace gene -I214V and G488S- and a 9bp deletion -Δ3Q. We revisited 11 Iberian locations to evaluate this adaptation of organophosphate (OP)-resistant alleles through amplicon sequencing. Screening for populations where the wild type is prevalent allows an identification of hotspots for targeted mitigation measures; we have hence refined the scale to the region with the lowest OP-resistant alleles frequency 71 locations were sampled and individuals checked using a fast and low-cost allele-specific-primer polymerase chain reaction (ASP-PCR) method]. An increase in Ace gene point-mutations was observed, and the Δ3Q mutation remains undetected. The lowest frequencies of the OP-resistant alleles remain in the west, underlining the hypothesis of an introduction of resistance from eastern Mediterranean areas. A field test was performed by sampling the fly population before and after in-practice dimethoate application. A clear reduction in olive fruit fly numbers was observed, with no relevant changes in the genotypic frequencies of the resistance alleles. The findings are discussed in frame of the type and intensity of the selection pressure that has led to the adaptation to resistance and its consequences from the producer perspective. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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Review

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22 pages, 534 KiB

Open AccessReview

Pest Management Challenges and Control Practices in Codling Moth: A Review

by Martina Kadoić Balaško, Renata Bažok, Katarina M. Mikac, Darija Lemic and Ivana Pajač Živković

Insects 2020, 11(1), 38; https://doi.org/10.3390/insects11010038 - 3 Jan 2020

Cited by 58 | Viewed by 7826

Abstract

The codling moth, Cydia pomonella L., is a serious insect pest in pome fruit production worldwide with a preference for apple. The pest is known for having developed resistance to several chemical groups of insecticides, making its control difficult. The control and management [...] Read more.

The codling moth, Cydia pomonella L., is a serious insect pest in pome fruit production worldwide with a preference for apple. The pest is known for having developed resistance to several chemical groups of insecticides, making its control difficult. The control and management of the codling moth is often hindered by a lack of understanding about its biology and ecology, including aspects of its population genetics. This review summarizes the information about the origin and biology of the codling moth, describes the mechanisms of resistance in this pest, and provides an overview of current research of resistant pest populations and genetic research both in Europe and globally. The main focus of this review is on non-pesticide control measures and anti-resistance strategies which help to reduce the number of chemical pesticides used and their residues on food and the local environment. Regular monitoring for insecticide resistance is essential for proactive management to mitigate potential insecticide resistance. Here we describe techniques for the detection of resistant variants and possibilities for monitoring resistance populations. Also, we present our present work on developing new methods to maintain effective control using appropriate integrated resistance management (IRM) strategies for this economically important perennial pest. Full article

(This article belongs to the Special Issue Monitoring and Detection of Insect Resistance)

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