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Biomanufacturing of Natural Bioactive Compounds

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 30 May 2024 | Viewed by 1180

Special Issue Editor


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Guest Editor
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China
Interests: bioactive compounds; sulfur metabolism; biosynthesis; podophylotoxin

Special Issue Information

Dear Colleagues,

Natural bioactive compounds are natural products with pharmacological activity produced by animal, plants, and microorganisms, such as podophyllotoxin, paclitaxel, and (-)-β-elemene. These compounds are commonly secondary metabolic products with complex structures and low yields, which make it difficult to obtain them as well as synthesize them chemically.

Biosynthesis, with the use enzymatic reactions, shows prominent advantage in the synthesis of natural bioactive products. Using cell factory or engineering enzyme to synthesize and catalyze the production of highly active natural products has broad prospects. The cellular uptake of special substrates, biosynthesis using enzymatic reactions with cloned gene (clusters) to improve yields or activity, and improved final products extraction methods are widely studied by many groups.

This Special Issue aims to provide a broad overview of the biosynthesis of natural bioactive compounds. Original research articles or reviews that discuss the transport of complicated substrates, expression of related genes, biosynthesis from simple substrate or derivation from prodrugs, and improved extraction methodologies of natural bioactive compounds as well as other related articles are welcome.

Dr. Honglei Liu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioactive compounds
  • natural products
  • biosynthesis
  • derivation
  • cell factory

Published Papers (1 paper)

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Research

23 pages, 1786 KiB  
Article
UHPLC-HRMS/MS Chemical Fingerprinting of the Bioactive Partition from Cultivated Piper aduncum L.
by Adélia Viviane de Luna, Thayssa da Silva Ferreira Fagundes, Ygor Jessé Ramos, Marlon Heggdorne de Araújo, Michelle Frazão Muzitano, Sanderson Dias Calixto, Thatiana Lopes Biá Ventura Simão, George Azevedo de Queiroz, Elsie Franklin Guimarães, André Mesquita Marques and Davyson de Lima Moreira
Molecules 2024, 29(8), 1690; https://doi.org/10.3390/molecules29081690 - 09 Apr 2024
Viewed by 870
Abstract
Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, flavonoids, and prenylated compounds. [...] Read more.
Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, flavonoids, and prenylated compounds. Extracts from P. aduncum have shown antibacterial, antifungal, and larvicidal activities. Our study explores the activity of extracts and partitions against Mycobacterium tuberculosis H37Rv, as well as the chemical diversity of the bioactive partition. This marks the first investigation of the bioactive partition of P. aduncum from agroecological cultivation. The ethyl acetate partition from the ethanolic leaf extract (PAEPL) was found to be the most active. PAEPL was subjected to column chromatography using Sephadex LH-20 and the obtained fractions were analyzed using UHPLC-HRMS/MS. The MS/MS data from the fractions were submitted to the online GNPS platform for the generation of the molecular network, which displayed 1714 nodes and 167 clusters. Compounds were identified via manual inspection and different libraries, allowing the annotation of 83 compounds, including flavonoids, benzoic acid derivatives, glycosides, free fatty acids, and glycerol-esterified fatty acids. This study provides the first chemical fingerprint of an antimycobacterial sample from P. aduncum cultivated in an agroecological system. Full article
(This article belongs to the Special Issue Biomanufacturing of Natural Bioactive Compounds)
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