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Molecules
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State-of-the-Art Analytical Technologies for Food Safety, Quality and Authenticity Assessment
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State-of-the-Art Analytical Technologies for Food Safety, Quality and Authenticity Assessment

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 675

Special Issue Editors

Dr. Marilena Dasenaki

E-Mail Website
Guest Editor
Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece
Interests: food analysis; food safety; chemical contaminants; mass spectrometry; food authenticity; bioactive molecules
Special Issues, Collections and Topics in MDPI journals
Dr. NIki Maragou

E-Mail Website
Guest Editor
Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece
Interests: food analysis; chromatography; mass spectrometry; food safety; quality control; regulatory legislation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Food safety, quality and authenticity are emerging topics of great interest for all  those involved in the food supply chain, starting from the food industry and producers to consumers, legal authorities and food science researchers. As it is well known, there are diverse factors that may affect the chemical composition of foods and the presence of contaminants such as the conditions of cultivation and breeding, geographical origin, variety, type of feed, processing, packaging, transportation and storage. Therefore, the implementation of sensitive, accurate, robust and efficient high-throughput analytical methodologies is needed to secure their safety and assess their quality and authenticity in terms of nutritional, organoleptic and bioactive characteristics. To address this imperative need, advanced analytical tools can be employed such as chromatographic techniques coupled to mass spectrometry, spectroscopic techniques, DNA-based methods and state-of-the-art omics approaches. In this context, this Special Issue will extensively cover the topics of application of state-of-the-art analytical techniques for food safety, quality and authenticity assessment. Scientists are warmly invited to submit their original contributions (reviews, original research papers and short communication) to this Special Issue, which will be of interest to a wide range of readers. In the case of review articles, an additional brief (1–2 pages) description of the topic including a draft index is required. This preliminary step is essential to avoid the overlap of topics.

Dr. Marilena Dasenaki
Dr. NIki Maragou
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food analysis
  • chemical contaminants
  • bioactive compounds
  • omics technologies
  • food characterization
  • mass spectrometry
  • NMR
  • spectroscopic techniques

Published Papers (1 paper)

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Research

11 pages, 2444 KiB  
Article
Novel and Sensitive Touchdown Polymerase Chain Reaction Assays for the Detection of Goat and Sheep Milk Adulteration with Cow Milk
by Ariadni Kourkouli, Nikolaos Thomaidis, Marilena Dasenaki and Athina Markou
Molecules 2024, 29(8), 1820; https://doi.org/10.3390/molecules29081820 - 17 Apr 2024
Viewed by 522
Abstract
Milk is the most consumed liquid food in the world due to its high nutritional value and relatively low cost, characteristics that make it vulnerable to adulteration. One of the most common types of milk adulteration involves the undeclared addition of cow’s milk [...] Read more.
Milk is the most consumed liquid food in the world due to its high nutritional value and relatively low cost, characteristics that make it vulnerable to adulteration. One of the most common types of milk adulteration involves the undeclared addition of cow’s milk to milk from other mammalian species, such as goats, sheep, buffalo or donkeys. The incidence of such adulteration not only causes a crisis in terms of commercial market and consumer uncertainty but also poses a risk to public health, as allergies can be triggered by proteins in undeclared cow’s milk. In this study, a specific qualitative touchdown (TD) PCR method was developed to detect the undeclared addition of cow’s milk in goat and sheep milk based on the discrimination of the peak areas of the melting curves after the modification of bovine-specific primers. The developed methodology has high specificity for the DNA templates of other species, such as buffalos and donkeys, and is able to identify the presence of cow’s milk down to 1%. Repeatability was tested at low bovine concentrations of 5% and 1% and resulted in %RSD values of 1.53–2.04 for the goat–cow assay and 2.49–7.16 for the sheep–cow assay, respectively. The application of this method to commercial goat milk samples indicated a high percentage of noncompliance in terms of labeling (50%), while a comparison of the results to rapid immunochromatographic and ELISA kits validated the excellent sensitivity and applicability of the proposed PCR methodology that was able to trace more adulterated samples. The developed assays offer the advantage of multiple detection in a single run, resulting in a cost- and time-efficient method. Future studies will focus on the applicability of these assays in dairy products such as cheese and yogurt. Full article
(This article belongs to the Special Issue State-of-the-Art Analytical Technologies for Food Safety, Quality and Authenticity Assessment)
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