Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
7.4
5.3
Journals
Nanomaterials
Special Issues
Stem Cells and Nanotechnology
share announcement

Stem Cells and Nanotechnology

A special issue of Nanomaterials (ISSN 2079-4991). This special issue belongs to the section "Biology and Medicines".

Deadline for manuscript submissions: closed (27 December 2020) | Viewed by 17689

Special Issue Editor

Prof. Dr. Claudia Giachino

E-Mail Website
Guest Editor
Department of Clinical and Biological Sciences, University of Turin, Orbassano, Turin, Italy
Interests: bio-nanotechnology; genetic stability; innovative therapy; regenerative medicine; stem cell biology

Special Issue Information

Dear Colleagues,

Stem cells and nanotechnology represent the tools of regenerative medicine, having the potential to provide new therapeutic availability and efficacy over conventional medicine. Indeed, the advent of nanotechnology and the discovery of the complex networks and stimuli that regulate cell fate processes have resulted in rapid advances in the healthcare scenario. The amalgamation of stem cell research with nanotechnology brings new prospects to stem cell therapy: it improves the specificity of the treatment, it controls stem cell proliferation, and it directs stem cell differentiation. Nanotechnology allows for the manipulation of highly advanced surfaces/scaffolds for optimal regulation of cellular behaviour; indeed, the role of nanoscale topography in scaffold design has gained much importance in regenerative medicine. Surely, the combination of stem cells with sophisticated nano-structured materials and scaffolds is going to be increasingly beneficial in all areas of regenerative medicine.

This Special Issue of Nanomaterials will publish high-quality research papers, short communications, and reviews covering the most recent advances from active researchers in the fields of nanomedicine, regenerative medicine, and bio-nanomaterials, concerning not only synthesis, preparation, and characterization, but especially focusing on applications of such innovative approaches in elevated medical-need settings. Techniques such as electrospinning, soft lithography, microfluidics, carbon nanotubes, and nanostructured hydrogel are relevant for this Special Issue, along with their potential usage in regenerative medicine. While the potential applications for nanotechnology in stem cell research are countless, stem cells and nanotechnology topics include, but are not limited to, the following three categories:

  • ŸTracking or labelling (visualizing stem cells using Fluorescent NPs, MRI and SPIO particles, QDs, etc.);
  • Delivery (applying nucleotide delivery for genetic control of stem cell fate, delivering chemicals or drugs, etc.);
  • Scaffold/platforms (optimizing the stem cell environment, etc.).

Prof. Dr. Claudia Giachino
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nanomaterials is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanomaterial
  • drug targeting
  • intelligent nanosystem
  • cell-nanoparticle interaction
  • cell tracking

Published Papers (5 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

11 pages, 3024 KiB  
Article
Behavior of Muscle-Derived Stem Cells on Silica Nanostructured Substrates
by Hyo-Sop Kim, Bit Na Lee, Sangdun Choi, Moon Suk Kim and Jae-Ho Kim
Nanomaterials 2020, 10(9), 1651; https://doi.org/10.3390/nano10091651 - 22 Aug 2020
Cited by 3 | Viewed by 1780
Abstract
The aim of the present work was to evaluate the responses of rat muscle-derived stem cells (rMDSCs) to growth on silica nanostructured substrates (SN) with nanoscale topographic surfaces. SN of different sizes (SN-60, SN-150, SN-300, SN-500, and SN-700) were prepared using silica nanoparticles [...] Read more.
The aim of the present work was to evaluate the responses of rat muscle-derived stem cells (rMDSCs) to growth on silica nanostructured substrates (SN) with nanoscale topographic surfaces. SN of different sizes (SN-60, SN-150, SN-300, SN-500, and SN-700) were prepared using silica nanoparticles with sizes of 60–700 nm. The prepared SN showed roughness at the nanoscale level. The total number of adherent cells on SN increased with increasing nanoscale level and incubation time. The rMDSCs attached to SN-500 and SN-700 were extensively flattened, whereas those grown on SN-60, SN-150, and SN-300 were more rounded. The rank order of the cell length and height of attached rMDSCs at 5 d on different surfaces was SN-60 ≈ SN-150 >> SN-300 > SN-500 > SN-700 > glass. Compared with rMDSCs grown on SN-60, SN-150, or SN-300, those attached to SN-500 and SN-700 exhibited a distinct morphology with filopodial extensions and stronger expression of focal adhesion, integrin, and actin. An evaluation of the gene expression of adhered rMDSCs showed that rMDSCs grown on SN-300 exhibited a higher environmental stress response than those grown on glass or SN-700. Collectively, our data provide fundamental insight into the cellular response and gene expression of rMDSCs grown on nanostructured substrates. Full article
(This article belongs to the Special Issue Stem Cells and Nanotechnology)
►▼ Show Figures

Figure 1

16 pages, 6123 KiB  
Article
Toxicity and Functional Impairment in Human Adipose Tissue-Derived Stromal Cells (hASCs) Following Long-Term Exposure to Very Small Iron Oxide Particles (VSOPs)
by Katrin Radeloff, Andreas Radeloff, Mario Ramos Tirado, Agmal Scherzad, Rudolf Hagen, Norbert H. Kleinsasser and Stephan Hackenberg
Nanomaterials 2020, 10(4), 741; https://doi.org/10.3390/nano10040741 - 13 Apr 2020
Cited by 7 | Viewed by 2395
Abstract
Magnetic nanoparticles (NPs), such as very small iron oxide NPs (VSOPs) can be used for targeted drug delivery, cancer treatment or tissue engineering. Another important field of application is the labelling of mesenchymal stem cells to allow in vivo tracking and visualization of [...] Read more.
Magnetic nanoparticles (NPs), such as very small iron oxide NPs (VSOPs) can be used for targeted drug delivery, cancer treatment or tissue engineering. Another important field of application is the labelling of mesenchymal stem cells to allow in vivo tracking and visualization of transplanted cells using magnetic resonance imaging (MRI). For these NPs, however, various toxic effects, as well as functional impairment of the exposed cells, are described. The present study evaluates the influence of VSOPs on the multilineage differentiation ability and cytokine secretion of human adipose tissue derived stromal cells (hASCs) after long-term exposure. Human ASCs were labelled with VSOPs, and the efficacy of the labelling was documented over 4 weeks in vitro cultivation of the labelled cells. Unlabelled hASCs served as negative controls. Four weeks after labelling, adipogenic and osteogenic differentiation was histologically evaluated and quantified by polymerase chain reaction (PCR). Changes in gene expression of IL-6, IL-8, VEGF and caspase 3 were determined over 4 weeks. Four weeks after the labelling procedure, labelled and unlabelled hASCs did not differ in the gene expression of IL-6, IL-8, VEGF and caspase 3. Furthermore, the labelling procedure had no influence on the multidifferentiation ability of hASC. The percentage of labelled cells decreased during in vitro expansion over 4 weeks. Labelling with VSOPs and long-term intracellular disposition probably have no influence on the physiological functions of hASCs. This could be important for the future in vivo use of iron oxide NPs. Full article
(This article belongs to the Special Issue Stem Cells and Nanotechnology)
►▼ Show Figures

Figure 1

8 pages, 3216 KiB  
Communication
Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
by Deepak Kumar, Stuart A. Cain and Lucy A. Bosworth
Nanomaterials 2019, 9(4), 522; https://doi.org/10.3390/nano9040522 - 3 Apr 2019
Cited by 6 | Viewed by 3195
Abstract
This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected [...] Read more.
This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected by both stimuli over a seven-day period. Cells were orientated parallel to the underlying fibre direction irrespective of environment (electrospun 2D fibre sheet or laminate 2D sheet with collagen gel layer). Application of cyclical tensile force (5% strain, 1 Hz, 1 h per day) encouraged hMSCs to remain at the fibre/gel interface, whereas cells cultured in static conditions migrated from the interface to the upper hydrogel layer. Depending on the stimulus applied, hMSCs presented an up-regulation in gene expression, indicative of several cell lineages, with those cultured at the interface and physically stimulated expressing markers indicative of angiogenesis, osteogenesis, and tenogenesis. This study highlights the importance of developing biomaterial scaffolds with environmental cues to specifically drive cells towards the tissue intended for bioengineering. Full article
(This article belongs to the Special Issue Stem Cells and Nanotechnology)
►▼ Show Figures

Graphical abstract

Review

Jump to: Research

28 pages, 2270 KiB  
Review
Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward
by Caterina Cristallini, Emanuela Vitale, Claudia Giachino and Raffaella Rastaldo
Nanomaterials 2020, 10(8), 1587; https://doi.org/10.3390/nano10081587 - 12 Aug 2020
Cited by 16 | Viewed by 3608
Abstract
To deliver on the promise of cardiac regeneration, an integration process between an emerging field, nanomedicine, and a more consolidated one, tissue engineering, has begun. Our work aims at summarizing some of the most relevant prevailing cases of nanotechnological approaches applied to tissue [...] Read more.
To deliver on the promise of cardiac regeneration, an integration process between an emerging field, nanomedicine, and a more consolidated one, tissue engineering, has begun. Our work aims at summarizing some of the most relevant prevailing cases of nanotechnological approaches applied to tissue engineering with a specific interest in cardiac regenerative medicine, as well as delineating some of the most compelling forthcoming orientations. Specifically, this review starts with a brief statement on the relevant clinical need, and then debates how nanotechnology can be combined with tissue engineering in the scope of mimicking a complex tissue like the myocardium and its natural extracellular matrix (ECM). The interaction of relevant stem, precursor, and differentiated cardiac cells with nanoengineered scaffolds is thoroughly presented. Another correspondingly relevant area of experimental study enclosing both nanotechnology and cardiac regeneration, e.g., nanoparticle applications in cardiac tissue engineering, is also discussed. Full article
(This article belongs to the Special Issue Stem Cells and Nanotechnology)
►▼ Show Figures

Graphical abstract

29 pages, 1781 KiB  
Review
Nanocarriers as Magic Bullets in the Treatment of Leukemia
by Mohammad Houshmand, Francesca Garello, Paola Circosta, Rachele Stefania, Silvio Aime, Giuseppe Saglio and Claudia Giachino
Nanomaterials 2020, 10(2), 276; https://doi.org/10.3390/nano10020276 - 6 Feb 2020
Cited by 40 | Viewed by 5962
Abstract
Leukemia is a type of hematopoietic stem/progenitor cell malignancy characterized by the accumulation of immature cells in the blood and bone marrow. Treatment strategies mainly rely on the administration of chemotherapeutic agents, which, unfortunately, are known for their high toxicity and side effects. [...] Read more.
Leukemia is a type of hematopoietic stem/progenitor cell malignancy characterized by the accumulation of immature cells in the blood and bone marrow. Treatment strategies mainly rely on the administration of chemotherapeutic agents, which, unfortunately, are known for their high toxicity and side effects. The concept of targeted therapy as magic bullet was introduced by Paul Erlich about 100 years ago, to inspire new therapies able to tackle the disadvantages of chemotherapeutic agents. Currently, nanoparticles are considered viable options in the treatment of different types of cancer, including leukemia. The main advantages associated with the use of these nanocarriers summarized as follows: i) they may be designed to target leukemic cells selectively; ii) they invariably enhance bioavailability and blood circulation half-life; iii) their mode of action is expected to reduce side effects. FDA approval of many nanocarriers for treatment of relapsed or refractory leukemia and the desired results extend their application in clinics. In the present review, different types of nanocarriers, their capability in targeting leukemic cells, and the latest preclinical and clinical data are discussed. Full article
(This article belongs to the Special Issue Stem Cells and Nanotechnology)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-5
Back to TopTop