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Liquid Chromatography in the Analysis of Bioactive Compounds for Pharmaceutical...
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Liquid Chromatography in the Analysis of Bioactive Compounds for Pharmaceutical Interest—3rd Edition

A special issue of Pharmaceuticals (ISSN 1424-8247). This special issue belongs to the section "Natural Products".

Deadline for manuscript submissions: closed (15 July 2024) | Viewed by 2090

Special Issue Editors

Prof. Dr. Jan Oszmianski

E-Mail Website
Guest Editor
Retired Professor, Wrocław, Poland
Interests: phenolic compounds; isolation and qualitative and quantitative determination of polyphenols; bioactive compounds; UV-VIS HPLC-MS-MS and SPE
Special Issues, Collections and Topics in MDPI journals
Dr. Sabina Lachowicz-Wiśniewska

E-Mail Website
Guest Editor
Faculty of Health Sciences, Department Food and Nutrition, Calisia University, 4 Nowy Świat Street, 62-800 Kalisz, Poland
Interests: nutraceuticals and functional foods; medicinal plants; bioactive compounds; HPLC; LC-MS; nutrition; antioxidant agent; prebiotics; probiotics; symbiotics; bioavailability in vitro
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Bioactive compounds, as natural primary and secondary metabolites, are used for the production of natural pharmaceuticals due to their pro-health properties. In order to confirm their identity, modern chromatographic techniques are used to allow for accurate quantitative and qualitative identification of the above-mentioned phytochemicals and their natural products. Liquid chromatography is one of the most efficient and robust specific techniques, due to the advantages of convenience and strong separation ability, as well as a wide range of material applications for identification. Liquid chromatography is widely used for the analysis of plants, nutraceuticals, pharmaceuticals, natural product quality control, or quantitative determination of bioactive compounds and others. The most commonly used methods for the identification of different plant materials and pharmaceuticals are ultra- and high-performance liquid chromatography with UV–VIS, fluorescence, and diode array, and are equipped with mass spectrometry or tandem mass spectrometry detection methods. It is important to mention that the latest scientific reports, also indicated in the 2nd edition of this Special Issue, indicate the significant analytical progress in pharmaceutical fields and that the application of liquid chromatography–mass spectrometry platforms for data acquisition is now common practice across the world. Therefore, for this Special Issue, we will publish studies concerning the latest scientific news, insights, and advances in the field of innovation of liquid chromatography for the analysis of bioactive compounds for pharmaceutical interest. Original research articles, short communications, and critical review articles dealing with the extraction and identification of bioactive compounds from natural matrices with biological and pharmaceutical interest are welcome. The information presented will arouse considerable interest among a large group of readers from different disciplines and research fields.

Prof. Dr. Jan Oszmianski
Dr. Sabina Lachowicz-Wiśniewska
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pharmaceuticals is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant materials
  • pharmaceuticals
  • product quality
  • innovative methods
  • polyphenolic compounds
  • isoprenoids
  • bioactive compounds
  • food chemistry
  • natural products
  • mass spectrometry
  • extraction
  • LC-MS/MS techniques
  • HPLC-DAD-MS

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Published Papers (2 papers)

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Research

14 pages, 2115 KiB  
Article
Phytochemical Profile, Antioxidant, Anti-Atopic, and Anti-Inflammatory Activities of Filipendula glaberrima Nakai at Different Growth Stages
by Hak-Dong Lee, Genevieve Tonog, Neil Patrick Uy, Yunji Lee, Ki-Young Kim, Hangeun Kim and Sanghyun Lee
Pharmaceuticals 2024, 17(7), 928; https://doi.org/10.3390/ph17070928 - 11 Jul 2024
Viewed by 960
Abstract
Since atopic dermatitis is an inflammatory skin disease, natural remedies, such as Filipendula glaberrima Nakai (FG), with anti-inflammatory properties are possible promising therapeutic options. This study aimed to investigate the therapeutic potential of FG extracts at different growth stages. Seven compounds were isolated [...] Read more.
Since atopic dermatitis is an inflammatory skin disease, natural remedies, such as Filipendula glaberrima Nakai (FG), with anti-inflammatory properties are possible promising therapeutic options. This study aimed to investigate the therapeutic potential of FG extracts at different growth stages. Seven compounds were isolated from the FG leaf extracts using open-column chromatography, and they were analyzed using HPLC. The extracts were further evaluated for their total polyphenol and flavonoid content (TPC and TFC). The in vitro antioxidant properties of the FG extracts were evaluated using radical scavenging assays, whereas their anti-inflammatory activities were assessed by evaluating their ability to inhibit the production of inflammation-associated biomarkers using the Griess assay and ELISA, respectively. The MTT assay was used to evaluate the viability and cytotoxicity of the FG extracts in keratinocyte cell lines. The results showed that the full-flowering stage exhibited the highest TPC, TFC, and antioxidant activities, thus suggesting a positive correlation between these properties. All FG extracts showed significant anti-inflammatory activity by inhibiting the production of pro-inflammatory biomarkers in lipopolysaccharide-stimulated macrophages. Additionally, the FG extracts suppressed the production of cytokines and chemokines in keratinocytes, indicating their anti-atopic potential. HPLC analysis revealed that the full-flowering stage had the highest content of all the analyzed phytochemicals (gallic acid, (+)-catechin, hyperin, miquelianin, astragalin, afzelin, and quercetin). These results suggest that the full-flowering stage of FG is the most promising source for therapeutic applications owing to its superior phytochemical profile and biological activities. This study highlights the potential of FG extracts, particularly in its full-flowering stage, as a natural therapeutic agent for the management of inflammation-related diseases, and it can also serve as a reference for further research on FG. Full article
(This article belongs to the Special Issue Liquid Chromatography in the Analysis of Bioactive Compounds for Pharmaceutical Interest—3rd Edition)
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20 pages, 1178 KiB  
Article
Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects
by Chang-Seob Seo, So-Yeon Kim and Dong-Seon Kim
Pharmaceuticals 2024, 17(6), 727; https://doi.org/10.3390/ph17060727 - 4 Jun 2024
Viewed by 624
Abstract
Yeokwisan (YWS) is an herbal medicine prescription consisting of six oriental herbal medicines, developed to treat reflux esophagitis. We focused on developing an analytical method capable of simultaneously quantifying 13 compounds in YWS samples using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) and ultra-performance [...] Read more.
Yeokwisan (YWS) is an herbal medicine prescription consisting of six oriental herbal medicines, developed to treat reflux esophagitis. We focused on developing an analytical method capable of simultaneously quantifying 13 compounds in YWS samples using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) and exploring their antioxidant effects. All compounds examined in both analytical systems were chromatographically separated on a SunFireTM C18 (4.6 × 250 mm, 5 μm) column and an Acquity UPLC BEH C18 (2.1 × 100 mm, 1.7 μm) column using gradient elution of a water–acetonitrile mobile phase. Antioxidant effects were evaluated based on radical scavenging activity (DPPH and ABTS tests) and ferrous ion chelating activity. In two analytical methods, the coefficient of determination of the regression equation was ≥0.9965, the recovery range was 81.11–108.21% (relative standard deviation (RSD) ≤ 9.33%), and the precision was RSD ≤ 11.10%. Application of the optimized analysis conditions gave quantitative analysis results for YWS samples of 0.02–100.36 mg/g. Evaluation of the antioxidant effects revealed that baicalein and baicalin exhibit significant antioxidant activity, suggesting that they play an important role in the antioxidant effects of YWS. Full article
(This article belongs to the Special Issue Liquid Chromatography in the Analysis of Bioactive Compounds for Pharmaceutical Interest—3rd Edition)
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