Dear Colleagues,

The area of optical microscopy and imaging technology is ever-evolving to extend the ability of researchers to elucidate intricate biological structures and functions. This is a key area in biology, biophysics, and medicine. There are continued and unmet needs for deeper, faster, and higher-resolution imaging across a multitude of biological specimens. Technology advances in photonics, optical systems, fluorescence labeling methods, and sample preparations continue to drive many important discoveries.

This Special Issue, entitled “Advanced Optical Microscopy and Imaging Technology”, aims to compile significant research and review articles on a wide range of optical microscopy technologies and imaging applications that reflect the current development and future directions of the field. We are pleased to invite you to submit your manuscripts to this Special Issue. Topics include, but are not limited to, the following:

• Advances in super-resolution microscopy: STED, single-molecule localization microscopy, structured illumination microscopy, MINSTED, MINFLUX, etc.;
• Developments in nonlinear optical microscopy: two-photon, three-photon fluorescence microscopy, second harmonic and third harmonic generation microscopy, stimulated Raman scattering microscopy;
• Technological advances and applications of light-sheet microscopy;
• Novel fluorescence labeling and sample preparation methods or applications: e.g.,: expansion microscopy, pan-expansion microscopy, exchangeable fluorescent labeling;
• Applications of adaptive optics in optical microscopy aberration correction;
• Progress and developments in confocal microscopy, computational imaging, and phase contrast imaging;
• Deep learning-aided microscopy implementation technology, for example, super-resolution microscopy, denoise, and aberration correction technology;
• Developments in multimodal optical correlative imaging.

We look forward to receiving your contributions.

Dr. Yang Li
Dr. Tong Ye
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• super-resolution microscopy
• expansion microscopy
• exchangeable fluorescent labeling
• light-sheet microscopy
• computational imaging
• structural illumination microscopy
• nonlinear optical microscopy
• deep learning