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Journals
Plants
Special Issues
In Vitro Techniques on Plant Propagation and Genetic Improvement
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In Vitro Techniques on Plant Propagation and Genetic Improvement

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 10 June 2024 | Viewed by 3113

Special Issue Editors

Dr. Hasan Mehraj

E-Mail Website
Guest Editor
Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan
Interests: heterosis; epigenetics; genotype to phenotype; brassica; plant tissue culture
Special Issues, Collections and Topics in MDPI journals
Dr. Monika Tuleja

E-Mail Website
Guest Editor
Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland
Interests: in vitro plant cultures; aspects of in vitro morphogenesis of selected species; including apomictic species; stress in vitro; the role of ethylene and other regulators of plant growth and development
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In vitro propagation is widely applied in various plants. It is a suitable way to ensure the genetic conservation of any endangered plant species or any other plant genetic resources. In vitro propagation in plants is highly species-specific and has been influenced by various factors such as carbon sources, plant growth regulators, light quality, and light intensity. Techniques such as callus culture, protoplast culture, organogenesis, and regeneration of protocorms like body (PLB), anther culture, ovary culture, and somatic embryogenesis are commonly used for in vitro plant propagation. In vitro propagation has also been extensively used for plant improvements through the application of embryo rescue, protoplast fusion, in vitro fertilization, and ploidy manipulation. Additionally, it has been used for genetic transformation and bioactive compounds or secondary metabolites production.

We aim to accumulate current updates on in vitro techniques in plants in this Special Issue. Therefore, we welcome manuscripts on in vitro plant propagation, crop improvement, bioactive compounds, genetic conservation, genetic and genomic studies, epigenetic and epigenomic studies, transcriptomics, etc.

Dr. Hasan Mehraj
Dr. Monika Tuleja
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • callus
  • somatic embryogenesis
  • PLB
  • plant growth regulators
  • light
  • morphogenetic response
  • secondary metabolites
  • stress reaction
  • plant improvement
  • transformation
  • DNA markers
  • genetics and genomics
  • epigenetics and epigenomics
  • transcriptomics

Published Papers (2 papers)

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Research

17 pages, 2633 KiB  
Article
In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower
by Marzena Nowakowska, Zaklina Pavlovic, Marcin Nowicki, Sarah L. Boggess and Robert N. Trigiano
Plants 2024, 13(2), 285; https://doi.org/10.3390/plants13020285 - 18 Jan 2024
Viewed by 824
Abstract
Helianthus verticillatus (Asteraceae), a whorled sunflower, is a perennial species restricted to a few locations in the southeastern United States and is now considered endangered. Therefore, restoring and protecting H. verticillatus as a species is a priority. This study introduces a highly efficient [...] Read more.
Helianthus verticillatus (Asteraceae), a whorled sunflower, is a perennial species restricted to a few locations in the southeastern United States and is now considered endangered. Therefore, restoring and protecting H. verticillatus as a species is a priority. This study introduces a highly efficient in vitro adventitious plant regeneration system from leaf explants, utilizing five diverse specimens of H. verticillatus, each representing distinct genotypes with phenotypic variations in leaf and stem morphology. Key factors influencing in vitro morphogenesis, including genetic constitution, explant source, and plant growth regulators (PGRs), were identified. The study revealed a remarkably strong genotype-dependent impact on the regeneration efficiency of the investigated H. verticillatus genotypes, ranging from a lack of regeneration to highly effective regeneration. The selection of two genotypes with varying regeneration abilities provides valuable models for genetic analyses, offering insights into factors influencing the regeneration potential of this endangered species. Optimum adventitious shoot regeneration results were achieved using Murashige and Skoog basal media (MS) supplemented with 8.8 µM N6-benzyladenine (BA) and 1.08 µM α-naphthalene acetic acid (NAA). This combination yielded the highest adventitious shoot production. Subsequent successful rooting on ½ MS medium without PGRs further solidified the efficiency of the developed protocol. Regenerated plantlets, demonstrating robust shoots and roots, were successfully acclimatized to greenhouse conditions with a 95% survival rate. The protocol developed in this study is the first such report for this endangered species and is expected to contribute to future genetic manipulation and modification studies. Full article
(This article belongs to the Special Issue In Vitro Techniques on Plant Propagation and Genetic Improvement)
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16 pages, 17481 KiB  
Article
A Large-Scale Validation of an Improved Embryo-Rescue Protocol for the Obtainment of New Table-Grape Seedless Genotypes
by Emanuele Chiaromonte, Giovanna Bottalico, Pierfederico Lanotte, Antonia Campanale, Vito Montilon, Massimo Morano, Antonia Saponari, Costantino Silvio Pirolo, Donato Gerin, Francesco Faretra, Stefania Pollastro and Vito Nicola Savino
Plants 2023, 12(19), 3469; https://doi.org/10.3390/plants12193469 - 3 Oct 2023
Viewed by 1450
Abstract
The new trends in the consumption of table grapes and the growing interest in the environmental impact of this crop have pushed breeders toward the development of seedless cultivars endowed with resistance, through crossbreeding programs. To obtain seedless grapes, the use of embryo-rescue [...] Read more.
The new trends in the consumption of table grapes and the growing interest in the environmental impact of this crop have pushed breeders toward the development of seedless cultivars endowed with resistance, through crossbreeding programs. To obtain seedless grapes, the use of embryo-rescue techniques is fundamental. In this research, a grape embryo-culture protocol was optimized and validated by using 39 cultivars and 41 cross-combinations carried out in the framework of a large private table grape program of the private network Italian Variety Club in the period 2017–2021 evaluating several factors, such as the improvement in embryo formation, germination and growth, and plantlet development. The embryo culture attitude of crosses between different combinations of seedless parents was assessed, and the rates of embryo development from the extracted ovules mostly ranged from 3.5 to 35.5% with 5 out of 43 genotypes outliers. Experiments conducted at different sampling times, in a range of 43–62 days after pollination (DAP), did not show significant differences between the samples analyzed, while the rate of embryos developed with the applied protocol proved its employability on multiple genotypes, although the grapevine genotype significantly influenced the technique efficiency. Full article
(This article belongs to the Special Issue In Vitro Techniques on Plant Propagation and Genetic Improvement)
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