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Research Progress in Electrochemical Aptasensors and Biosensors
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Research Progress in Electrochemical Aptasensors and Biosensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 3835

Special Issue Editors

Dr. Luisa Pilan

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Guest Editor
Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 7 Gheorghe Polizu, 011061 Bucharest, Romania
Interests: electrochemical biosensors; electrochemical functionalization; carbon nanomaterials; conductive polymers; supercapacitors; corrosion
Special Issues, Collections and Topics in MDPI journals
Dr. Matei D. Raicopol

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Guest Editor
Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 7 Gheorghe Polizu, 011061 Bucharest, Romania
Interests: electrochemical biosensors; electrochemical functionalization; carbon nanomaterials; conductive polymers; supercapacitors; corrosion

Special Issue Information

Dear Colleagues,

The need for rapid and precise monitoring and detection of various analytes, such as clinical biomarkers or food and environmental contaminants, has considerably sped up the development of biosensor technology. In this context, electrochemical transduction is currently one of the main strategies applied in biosensing, because electrochemical biosensors can combine the sensitivity of electroanalytical detection with the high specificity that biorecognition elements display for their targets. Furthermore, recent improvements in sequencing methodologies and computational methods provide important support in developing effective engineered bioreceptors, such as aptamers.

This Special Issue, "Research Progress in Electrochemical Aptasensors and Biosensors", welcomes original research papers and reviews addressing recent advances in electrochemical biosensing technology, including, but not limited to: synthesis of new electrode materials and engineering of their functional properties, integration of nanomaterials towards electrochemical signal amplification in biosensors, and fabrication and performance evaluation of new biosensing devices based on natural or synthetic biorecognition elements.

Dr. Luisa Pilan
Dr. Matei D. Raicopol
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemical biosensors
  • aptasensors
  • DNA biosensors
  • immunosensors
  • enzyme biosensors
  • nanotechnologies for biosensors
  • functional electrode materials
  • molecular-imprinted polymers
  • healthcare
  • food control
  • environmental monitoring
  • lab-on-a-chip
  • point-of-care

Published Papers (3 papers)

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Research

12 pages, 3287 KiB  
Article
Hydrogen Peroxide and Dopamine Sensors Based on Electrodeposition of Reduced Graphene Oxide/Silver Nanoparticles
by Yuhang Zhang, Na Li, Bo Liu and Hangyu Zhang
Sensors 2024, 24(2), 355; https://doi.org/10.3390/s24020355 - 07 Jan 2024
Viewed by 773
Abstract
In this work, silver nanoparticles (AgNPs)/reduced graphene oxide (rGO) nanocomposites were electrodeposited on glassy carbon electrodes (GCE) to construct electrochemical sensors for the detection of hydrogen peroxide (H2O2) and dopamine (DA). The AgNPs were synthesized on graphene oxide (GO) [...] Read more.
In this work, silver nanoparticles (AgNPs)/reduced graphene oxide (rGO) nanocomposites were electrodeposited on glassy carbon electrodes (GCE) to construct electrochemical sensors for the detection of hydrogen peroxide (H2O2) and dopamine (DA). The AgNPs were synthesized on graphene oxide (GO) by the hydrothermal method, followed by the reduction of the GO during the electrodeposition process, resulting in the formation of the nanocomposites on the surface of the electrodes. The generation of AgNPs on the graphene sheets was verified by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The AgNPs/rGO/GCE showed a linear response to H2O2 in the range of 5 μM to 620 μM, with a sensitivity of 49 μA mM−1cm−2 and a limit of detection (LOD) of 3.19 μA. The linear response of the AgNPs/rGO/GCE to DA ranged from 1 μM to 276 μM, the sensitivity was 7.86 μA mM−1cm−2, and the LOD was 0.18 μM. Furthermore, DA and H2O2 were detected simultaneously in the same solution without interferences, and the sensors displayed good stability over time. The preparation method for the sensors is relatively eco-friendly, convenient, and efficient, exhibiting great potential for sensitive detection of DA and H2O2. Full article
(This article belongs to the Special Issue Research Progress in Electrochemical Aptasensors and Biosensors)
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17 pages, 7422 KiB  
Article
Effect of Ambient Environment on Laser Reduction of Graphene Oxide for Applications in Electrochemical Sensing
by Abdullah A. Faqihi, Neil Keegan, Lidija Šiller and John Hedley
Sensors 2023, 23(18), 8002; https://doi.org/10.3390/s23188002 - 21 Sep 2023
Viewed by 928
Abstract
Electrochemical sensors play an important role in a variety of applications. With the potential for enhanced performance, much of the focus has been on developing nanomaterials, in particular graphene, for such sensors. Recent work has looked towards laser scribing technology for the reduction [...] Read more.
Electrochemical sensors play an important role in a variety of applications. With the potential for enhanced performance, much of the focus has been on developing nanomaterials, in particular graphene, for such sensors. Recent work has looked towards laser scribing technology for the reduction of graphene oxide as an easy and cost-effective option for sensor fabrication. This work looks to develop this approach by assessing the quality of sensors produced with the effect of different ambient atmospheres during the laser scribing process. The graphene oxide was reduced using a laser writing system in a range of atmospheres and sensors characterised with Raman spectroscopy, XPS and cyclic voltammetry. Although providing a slightly higher defect density, sensors fabricated under argon and nitrogen atmospheres exhibited the highest average electron transfer rates of approximately 2 × 10−3 cms−1. Issues of sensor reproducibility using this approach are discussed. Full article
(This article belongs to the Special Issue Research Progress in Electrochemical Aptasensors and Biosensors)
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13 pages, 3120 KiB  
Article
Disposable Sensor Chips with Molecularly Imprinted Carbon Paste Electrodes for Monitoring Anti-Epileptic Drugs
by Aaryashree, Ashish Kumar Choudhary and Yasuo Yoshimi
Sensors 2023, 23(6), 3271; https://doi.org/10.3390/s23063271 - 20 Mar 2023
Cited by 1 | Viewed by 1756
Abstract
Epilepsy is a neurological disorder that affects millions of people worldwide. Anti-epileptic drugs (AEDs) are critical for their management. However, the therapeutic window is narrow, and traditional laboratory-based therapeutic drug monitoring (TDM) methods can be time consuming and unsuitable for point-of-care testing. To [...] Read more.
Epilepsy is a neurological disorder that affects millions of people worldwide. Anti-epileptic drugs (AEDs) are critical for their management. However, the therapeutic window is narrow, and traditional laboratory-based therapeutic drug monitoring (TDM) methods can be time consuming and unsuitable for point-of-care testing. To address this issue, we developed a disposable sensor chip based on molecularly imprinted polymer-modified carbon paste electrodes (MIP-CPs) for the TDM of AEDs such as phenobarbital (PB), carbamazepine (CBZ), and levetiracetam (LEV). In this work, functional monomers (methacrylic acid) and crosslinking monomers (methylene bisacrylamide and ethylene glycol dimethacrylate) were copolymerized in the presence of the AED template and grafted on the graphite particles by simple radical photopolymerization. The grafted particles were mixed with silicon oil, dissolving ferrocene as a redox marker to make the MIP-carbon paste (CP). Disposable sensor chips were fabricated by packing the MIP-CP into the base made of poly (ethylene glycol terephthalate) (PET) film. The sensor’s sensitivity was determined using differential pulse voltammetry (DPV), carried out on a single sensor chip for each operation. Linearity was obtained from 0–60 μg/mL in PB and LEV and 0–12 μg/mL in CBZ, covering their respective therapeutic range. The time taken for each measurement was around 2 min. The experiment using whole bovine blood and bovine plasma indicated that the existence of species that interfered had a negligible effect on the test’s sensitivity. This disposable MIP sensor provides a promising approach for point-of-care testing and facilitating the management of epilepsy. Compared with existing tests, this sensor offers a faster and more accurate way to monitor AEDs, which is crucial for optimizing therapy and improving patient outcomes. Overall, the proposed disposable sensor chip based on MIP-CPs represents a significant advancement in AED monitoring, with the potential for rapid, accurate, and convenient point-of-care testing. Full article
(This article belongs to the Special Issue Research Progress in Electrochemical Aptasensors and Biosensors)
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