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Separations
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Advances in the Chromatographic Separation and Analysis of Natural Products/Medicines
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Advances in the Chromatographic Separation and Analysis of Natural Products/Medicines

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 2893

Special Issue Editors

Dr. Xingchu Gong

E-Mail Website
Guest Editor
1. Pharmaceutical Informatics Institute, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China
2. Jinhua Institute of Zhejiang University, Jinhua 321016, China
Interests: traditional Chinese medicine quality control; pharmaceutical engineering
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Shengqiang Tong

E-Mail Website
Guest Editor
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China
Interests: chromatography; pharmaceutical analysis
Dr. Guanglei Zuo

E-Mail Website
Guest Editor
Jinhua Institute of Zhejiang University, Jinhua 321016, China
Interests: natural product chemistry; high-speed countercurrent chromatography; separation and identification; enzyme inhibitors

Special Issue Information

Dear Colleagues,

Natural products have been used for centuries as a source of medicine, and modern analytical techniques have enabled the identification and characterization of their active ingredients. Chromatography is a key technique used for the separation and purification of these compounds, and recent advances in instrumentation and methodology have improved the efficiency and sensitivity of these processes.

This Special Issue will focus on the latest developments in the chromatographic separation and analysis of natural products and medicines, which will cover a range of topics related to the chromatographic separation and analysis of natural products and medicines. Articles will explore the use of various chromatographic techniques, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and high-speed counter-current chromatography (HSCCC), and supercritical fluid chromatography (SFC), for the separation and purification of natural compounds. Additionally, the Special Issue will highlight the latest developments in sample preparation, detection methods, and separation processes.

The articles in this Special Issue will be of interest to researchers and practitioners in the fields of natural product chemistry, pharmaceuticals, and analytical chemistry. They will provide valuable insights into the latest advances in chromatographic separation and analysis techniques for natural products and medicines, with potential applications in drug discovery, quality control, and clinical research.

Dr. Xingchu Gong
Prof. Dr. Shengqiang Tong
Dr. Guanglei Zuo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography separation process
  • natural products
  • medicinal plants
  • phytochemical analysis
  • bioactive compounds

Published Papers (2 papers)

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Research

12 pages, 1596 KiB  
Article
Unveiling Novel Chaotropic Chromatography Method for Determination of Pralidoxime in Nerve Agent Antidote Autoinjectors
by Bohyun Shin, Hyung-seung Kim, Ji-Youn Lee, Sumin Seo, Cho Hee Jeong, Eunbin Bae, Jiyu Kim, Hyojeong Lee, Donghee Lee, Dong-Kyu Lee and Sang Beom Han
Separations 2024, 11(3), 82; https://doi.org/10.3390/separations11030082 - 7 Mar 2024
Viewed by 992
Abstract
Pralidoxime chloride, a highly hydrophilic antidote, cannot be effectively separated by reverse-phase high-performance liquid chromatography (RP-HPLC), unless the mobile-phase composition is varied. However, the use of ion-pairing reagents for pralidoxime separation is hindered by the persistent contamination of the stationary phase or chromatography [...] Read more.
Pralidoxime chloride, a highly hydrophilic antidote, cannot be effectively separated by reverse-phase high-performance liquid chromatography (RP-HPLC), unless the mobile-phase composition is varied. However, the use of ion-pairing reagents for pralidoxime separation is hindered by the persistent contamination of the stationary phase or chromatography system inside the HPLC system. Thus, this study aimed to develop a simple, rapid, and robust method based on RP-HPLC to determine pralidoxime chloride in antidote autoinjectors using a chaotropic salt as the mobile-phase additive. The use of UV detection at 270 nm allowed for the simultaneous detection of pralidoxime chloride and the internal standard, pyridine-2-aldoxime. The addition of chaotropic salts (NaPF6, NaBF4, and NaClO4) and an ionic liquid ([EMIM]PF6) increased the retention time of pralidoxime chloride. Among them, NaPF6 exhibited the highest capacity factor in the reverse-phase C18 column. Increasing the salt concentration increased the capacity factor and the number of theoretical plates. Analytical method validation was performed to assess the linearity, accuracy, precision, recovery, and repeatability, according to the Ministry of Food and Drug Safety guidelines. Additionally, this newly developed method exhibits an adequate separation capability, making it a potential substitute for the current method employed in the United States/Korean Pharmacopoeia, and it ensures the necessary durability to maintain the robustness and reliability of the analytical system. Full article
(This article belongs to the Special Issue Advances in the Chromatographic Separation and Analysis of Natural Products/Medicines)
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13 pages, 2364 KiB  
Article
Analysis of Volatile Components and Antibacterial Activity of Silver Wormwood Essential Oils from Different Habitats by E-Nose Combined with GC-MS
by Yiqin Yang, Dongyun Guo, Shujie Yang, Huiquan Hu, Xiaorong Luo and Na Wan
Separations 2023, 10(11), 553; https://doi.org/10.3390/separations10110553 - 31 Oct 2023
Viewed by 1566
Abstract
Electronic nose (E-nose) combined with gas chromatography–mass spectrometry (GC-MS) was used to analyze the volatile components of silver wormwood from different habitats, and the antibacterial activity of essential oils was also studied, to provide a scientific basis for quality control of silver wormwood [...] Read more.
Electronic nose (E-nose) combined with gas chromatography–mass spectrometry (GC-MS) was used to analyze the volatile components of silver wormwood from different habitats, and the antibacterial activity of essential oils was also studied, to provide a scientific basis for quality control of silver wormwood and rational utilization of their essential oils. In this study, the total content of essential oils in silver wormwood was determined by steam distillation; the volatile components were conducted in an overall analysis by E-nose combined with chemometrics; the volatile components were analyzed and identified by GC-MS; and two G-negative bacteria and one Gram-positive bacteria were used as test bacteria to determine the antibacterial activity of the essential oils from silver wormwood. The results showed that principal component analysis (PCA) and linear discriminant analysis (LDA) of E-nose could distinguish the essential oils of silver wormwood from different habitats, and the odor difference of essential oils was obvious. A total of 87 volatile components were identified by GC-MS, and there were significant differences in components and contents in silver wormwood from different habitats; PCA and hierarchical cluster analysis (HCA) could effectively distinguish silver wormwood from different habitats. The essential oils from silver wormwood from different habitats all had a certain inhibitory effect on Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. Therefore, the combination of E-nose and GC-MS could quickly distinguish silver wormwood from different habitats and provide a reference for quality control, drug selection, and comprehensive utilization of silver wormwood. Full article
(This article belongs to the Special Issue Advances in the Chromatographic Separation and Analysis of Natural Products/Medicines)
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