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Separations
Special Issues
Mass Spectrometry Development and Its Application in Bioanalysis
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Mass Spectrometry Development and Its Application in Bioanalysis

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Bioanalysis/Clinical Analysis".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 3121

Special Issue Editors

Prof. Dr. Athula Attygalle

E-Mail Website
Guest Editor
Department of Chemistry and Chemical Biology, Stevens Institute of Technology, Hoboken, NJ 07030, USA
Interests: mass spectrometry; mass spectrometric ion fragmentations; bioanalytical chemistry; microchemical techniques; chromatography; ion mobility separations
Dr. Julius Pavlov

E-Mail Website
Guest Editor
Department of Chemistry and Chemical Biology, Stevens Institute of Technology, Hoboken, NJ 07030, USA
Interests: mass spectrometry

Special Issue Information

Dear Colleagues,

Mass spectrometry-based techniques are among the leading methods applied to characterize and quantify various biologically relevant small and large biomolecules such as sugars, lipids, proteins, and oligonucleotides. It is a rapidly developing, highly dynamic field. Novel developments are continuously added. Particularly, when combined with chromatographic and ion-mobility separation, mass spectrometry provides a method unrivaled by any other analytical method in terms of versatility, speed of sample analysis, and limits of detectability. This Special Issue aims to collect original research papers and review articles on novel developments in mass spectrometry-based techniques for biological analysis.

Prof. Dr. Athula Attygalle
Dr. Julius Pavlov
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • mass spectrometry
  • biomolecules
  • chromatographic separation
  • ion-mobility separation
  • biological analysis

Published Papers (1 paper)

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Research

17 pages, 1944 KiB  
Article
Salting-Out Assisted Liquid-Liquid Extraction for UPLC-MS/MS Determination of Thyroxine and Steroid Hormones in Human Serum and Fish Plasma
by Alemnesh Yirda Urge, Daniela Maria Pampanin, Maria Elena Martino, David Lausten Knudsen and Cato Brede
Separations 2023, 10(4), 240; https://doi.org/10.3390/separations10040240 - 5 Apr 2023
Cited by 2 | Viewed by 2852
Abstract
Measuring the level of steroid and thyroxine hormones is key to understanding organism health conditions. Liquid chromatography coupled with tandem mass spectrometry has become the method of choice for such hormone analyses in clinical laboratories. Detection of hormones at low levels typically requires [...] Read more.
Measuring the level of steroid and thyroxine hormones is key to understanding organism health conditions. Liquid chromatography coupled with tandem mass spectrometry has become the method of choice for such hormone analyses in clinical laboratories. Detection of hormones at low levels typically requires a time-consuming sample preparation, such as liquid-liquid extraction followed by solvent evaporation and re-solubilization of the sample extract. Instead, we applied salting-out assisted liquid-liquid extraction (SALLE) for the extraction of thyroxine, testosterone, cortisone, and cortisol from human serum and fish plasma samples. SALLE allowed direct injection of sample extracts. Sodium chloride and ammonium sulfate were evaluated as salting-out reagents together with four different organic solvents. High extraction recovery and reduced matrix interference were achieved by using ammonium sulfate together with 10% methanol in acetonitrile. Limits of quantification were in the range of 0.1–0.2 ng/mL and signal responses were linear (R2 > 0.997) up to at least 100 ng/mL for all hormones. The method was applied for hormone measurements in fish plasma. In conclusion, SALLE combines the simplicity of crude protein precipitation with the high analyte enrichment of a liquid-liquid extraction. Here we have presented it as a novel sample preparation method for clinical laboratories where mass spectrometry is utilized in the field of endocrinology. Full article
(This article belongs to the Special Issue Mass Spectrometry Development and Its Application in Bioanalysis)
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