HPLC or Other Chromatographic-Based Methods for Analysis of Bioactive Compounds in Plant, Food, and Pharmaceutical Products

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: 10 May 2024 | Viewed by 1485

Special Issue Editor


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Guest Editor
Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea
Interests: isolation; separation; purification of bioactive compounds

Special Issue Information

Dear Colleagues,

Bioactive compounds are substances that enhance or inhibit the functions of living organisms, playing a role in correcting abnormal conditions due to deficiencies in or the excessive secretion of substances regulating functions in the living body. Therefore, the creation of physiologically active substances is very important for helping modern people to lead a better and healthier life, and extensive research has been conducted on this subject recently. New biologically active substances can be obtained from natural products such as animals and plants, extracted and purified from metabolites of microorganisms and animal and plant cell lines, or obtained through chemical synthesis. There are various pretreatment methods available to separate and purify these bioactive substances, and HPLC is a representative analytical method.

In this Issue, we would like to introduce various pretreatment methods for extraction and purification of bioactive substances from plant, food or pharmaceuticals and analysis methods using HPLC and other chromatography.

Prof. Dr. Yang-Bong Lee
Guest Editor

Manuscript Submission Information

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Keywords

  • bioactive compounds
  • physiologically active substances
  • medicinal substances
  • natural products
  • metabolites
  • pretreatment methods
  • extraction, isolation, separation and purification
  • plant, food and pharmaceutical compounds
  • analytical methods
  • HPLC
  • other chromatographic technologies

Published Papers (1 paper)

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Research

14 pages, 2306 KiB  
Article
HPLC/DAD Analysis and Antioxidant Activity of Adlay Sprouts and Seeds
by Gia-Han Tran, Neil-Patrick Uy, Shi-Heon Kang, Wonseok Heo, Eun-Song Lee, Seong-Soo Roh and Sanghyun Lee
Separations 2024, 11(1), 32; https://doi.org/10.3390/separations11010032 - 13 Jan 2024
Cited by 2 | Viewed by 1312
Abstract
Adlay is an annual plant known for its abundant bioactive compounds and diverse pharmacological activities. Coixol, a key component found in various parts of adlay, significantly contributes to its biological activity. This study was conducted to extract adlay sprouts and seeds using different [...] Read more.
Adlay is an annual plant known for its abundant bioactive compounds and diverse pharmacological activities. Coixol, a key component found in various parts of adlay, significantly contributes to its biological activity. This study was conducted to extract adlay sprouts and seeds using different solvents (methanol and ethanol) and extraction methods (reflux and ultrasonic extraction). The extracts were then evaluated for their total polyphenol and flavonoid contents, as well as antioxidant ability (DPPH and ABTS+). Additionally, the coixol content of these extracts was analyzed using HPLC/DAD analysis. The results showed that the extraction methods and solvents used impacted the bioactive compounds and their activities in the samples. Adlay sprouts exhibited a higher compound content and stronger antioxidant capacity than adlay seeds. Moreover, a substantial amount of coixol was found in the sprouts, while it was not detected in the seeds. This study emphasizes the importance of selecting appropriate extraction methods to optimize the biological activities of adlay sprouts and seeds. Adlay sprouts, with their enriched phytochemical compounds and enhanced antioxidant ability, could serve as a valuable material for health product applications. Full article
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