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Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice

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A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Surgery".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 14217

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Special Issue Editors

Dr. Gerardo Fatone

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Guest Editor

Department of Veterinary Medicine and Animal Production, University of Naples “Federico II”, Via Federico Delpino 1, 80137 Naples, Italy
Interests: general surgery; orthopedic and neurosurgery; small animal and ruminants
Special Issues, Collections and Topics in MDPI journals

Dr. Giovanni Della Valle

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Guest Editor

Special Issue Information

Dear Colleagues,

Regenerative medicine is an interdisciplinary field of clinical research and therapy focused on the repair, replacement, or regeneration of cells, tissues, and organs, with the goal of restoring the structure and function of the affected tissue or organ.

The clinical goals of regenerative medicine include reducing inflammation and pain, improving the healing of injured tissues, and, finally, returning function. Methods to achieve these goals vary widely among the various regenerative medicine treatment modalities. This Special Issue, "Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice", aims to address wound management at each stage of wound healing, with a primary focus on new strategies aiming to alleviate inflammation and pain, reduce debridement and products stimulating granulation tissue, and improve epithelialization and healing of injured tissues both in small and large animals. The secondary focus is addressing the state of the art in wound management in large animals, and the development of new plans of action to reduce the use of systemic or topical antibiotics in the field.

Several well-known scientists in this area have expressed their interest in participating in this project. We are pleased to invite you to contribute to this Special Issue of Veterinary Sciences.

Original research articles, case report, case series and reviews are welcome. Research areas may include (but are not limited to): small animal and large animal wound management; stem cell therapy; tissue engineering; gene therapy; and the use of autologous patient-derived bioscaffolds.

We look forward to receiving your contributions.

Dr. Gerardo Fatone
Dr. Giovanni Della Valle
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

canine cutaneous wounds
regenerative medicine
ruminant cutaneous wound
skin lesion
bioscaffold
platelet derivates
stem cells
wound management
wound healing
granulation
debridement technique
epithelialization
interdigital lesions
soft tissue repair

Published Papers (6 papers)

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Research

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35 pages, 12154 KiB

Open AccessArticle

First-Intention Incisional Wound Healing in Dogs and Cats: A Controlled Trial of Dermapliq and Manuka Honey

by Pagona G. Gouletsou, Theodora Zacharopoulou, Vassilis Skampardonis, Stefanos G. Georgiou, Dimitrios Doukas, Apostolos D. Galatos, Eugenia Flouraki, Eleftheria Dermisiadou, Chryssoula Margeti, Mariana Barbagianni, Aikaterini Sideri and Vassiliki Tsioli

Vet. Sci. 2024, 11(2), 64; https://doi.org/10.3390/vetsci11020064 - 1 Feb 2024

Viewed by 1817

Abstract

This study aimed to compare incisional wound healing in cats and dogs after the topical application of Μanuka honey and a new medical device, Dermapliq. Comparisons were made between each treatment and control, between the two treatments, and between dogs and cats. Twelve cats and twelve dogs were included in this study, and the impact of the two substances was examined through cosmetic, clinical, ultrasonographical, and histological evaluation. The use of Dermapliq in first-intention wound healing achieved a significantly better cosmetic evaluation score and better total clinical score at days 20–41, compared to the control, in both dogs and cats. The ultrasonographically estimated wound area was smaller with Dermapliq compared to the control. Wounds treated with Dermapliq showed histologically less inflammation compared to the control. The use of Manuka honey did not show a significantly better cosmetic score compared to the control. Skin thickening was significantly higher after using Manuka honey compared to the control and so was the total clinical score. However, the median wound area, as was evaluated ultrasonographically, was significantly smaller when wounds were treated with Manuka honey, the difference being more apparent in dogs. Dermapliq was proven to be a better choice in achieving favorable wound healing than Manuka honey in dogs and cats in first-intention healing. In our study, cats had a statistically better cosmetic score and less skin thickening and scar width compared to dogs. Histologically, cats showed significantly less edema, higher inflammation and angiogenesis scores, and lower fibroblast and epidermis thickening scores when compared to dogs. Full article

(This article belongs to the Special Issue Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice)

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15 pages, 6852 KiB

Open AccessArticle

Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft

by Radina Vasileva and Tzvetan Chaprazov

Vet. Sci. 2023, 10(3), 196; https://doi.org/10.3390/vetsci10030196 - 5 Mar 2023

Cited by 1 | Viewed by 1745

Abstract

Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone. Full article

(This article belongs to the Special Issue Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice)

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26 pages, 29275 KiB

Open AccessArticle

Comparison of Absorbable and Nonabsorbable Sutures for Intradermal Skin Closure in Dogs

by Dimitrios B. Balomenos, Pagona G. Gοuletsοu and Apostolos D. Galatos

Vet. Sci. 2023, 10(2), 105; https://doi.org/10.3390/vetsci10020105 - 1 Feb 2023

Cited by 1 | Viewed by 4349

Abstract

The study aimed to compare incisional wound healing with intradermal suture patterns performed with (a) absorbable suture with burying of the knots and (b) nonabsorbable suture anchored with clips. Ten dogs were included in the study. Surgically created skin incisions were apposed with continuous intradermal suture pattern with 4/0 poliglecaprone 25 with burying of the knots and continuous intradermal pattern with 4/0 polypropylene with clips. Cosmetic, clinical, ultrasonographic and histological scores were evaluated. The intradermal pattern with clips was easier to perform and required significantly less time to complete than the intradermal suture with burying of the knots. Cosmetic, clinical, ultrasonographic and histological evaluation scores did not differ significantly between the techniques. Irrespective of the technique used, the cosmetic, ultrasonographic, clinical and histological appearances of the incisions improved over time. In conclusion, polypropylene was found to be a safe and effective suture material for use with intradermal suture pattern with clips in dogs and to have an easy and quick application. However, in our sample, its earlier removal from wounds than poliglecaprone 25 was not found to be associated with a supposedly beneficial effect on wound healing and scar appearance. Both suture materials can be useful in intradermal suture techniques in dogs. Full article

(This article belongs to the Special Issue Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice)

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10 pages, 5147 KiB

Open AccessArticle

Diagnostic Imaging Studies on Local and Systemic Erythropoietin Application for Promoting Bone Regeneration in Rat Calvarial Defects

by Tsvetan Chaprazov, Radina Vasileva, Kiril Atliev and Elena Firkova

Vet. Sci. 2022, 9(10), 578; https://doi.org/10.3390/vetsci9100578 - 18 Oct 2022

Cited by 1 | Viewed by 1668

Abstract

The purpose of this study was to compare the effects of local and systemic application of recombinant human erythropoietin (rhEPO) on the healing of rat calvarial defects. Twenty-four male skeletally-mature Wistar rats were used. Two bone 5 mm critical size defects were created in calvarial bones of each rat. In rats from experimental group I (n = 12), EPO was applied locally on a collagen cone in left defects, whereas a collagen cone soaked with physiological saline was placed in right defects. The rats from experimental group II were injected once intraperitoneally with 4900 IU/kg EPO; a collagen cone was only placed in left defects, whereas the right defects were left empty. The systemic effect of EPO treatment was monitored by haematological analyses on days 0, 30 and 90. Bone healing was monitored via radiography and computed tomography on the same time intervals. The results demonstrated that local EPO application had no significant effect on haemopoiesis, unlike the systemic application. At the same time, it resulted in new bone formation and therefore, could be successfully used as a means of promoting bone regeneration. Full article

(This article belongs to the Special Issue Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice)

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13 pages, 1312 KiB

Open AccessArticle

In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing

by Ravisa Warin, Preeyanat Vongchan, Witaya Suriyasathaporn, Ratchadaporn Boripun and Wanna Suriyasathaporn

Vet. Sci. 2022, 9(10), 566; https://doi.org/10.3390/vetsci9100566 - 15 Oct 2022

Cited by 1 | Viewed by 1976

Abstract

Advanced platelet-rich fibrin (A-PRF) induces more proliferation and migration of fibroblasts compared with standard PRF, but it being freshly prepared prior to it being applied is necessary. Therefore, this study aimed to determine the effect of lyophilized A-PRF on growth factor release and cell biological activity. Blood samples were collected from six dogs and processed for fresh and lyophilized A-PRF. The growth factors released included transforming growth factor beta-1 (TGF-β1), vascular endothelial growth factor-A (VEGFA), and platelet-derived growth factor-BB (PDGF-BB), and the fibroblast proliferation as well as wound closure enhancement of both products were compared. The results showed that TGF-β1, PDGF-BB, and VEGFA were continually released from lyophilized A-PRF for over 72 h. Lyophilized A-PRF released significantly more accumulated VEGEA and a tendency to release more TGF-β1 at 72 h as well as VEGFA at 24 h and 72 h than fresh A-PRF. Moreover, lyophilized A-PRF increased fibroblast proliferation and induced a significantly faster wound closure than the control, while no significant difference between fresh and lyophilized A-PRF was found. In conclusion, the lyophilization of canine A-PRF can preserve the release of growth factors and has similar biological activities to a fresh preparation. This encourages the substitution of lyophilized A-PRF instead of fresh A-PRF in regenerative treatments in which the stability of the product is concerned. Full article

(This article belongs to the Special Issue Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice)

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Review

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14 pages, 1027 KiB

Open AccessReview

Clinical Application of Platelet Concentrates in Bovine Practice: A Systematic Review

by Chiara Caterino, Giovanni Della Valle, Federica Aragosa, Stefano Cavalli, Jacopo Guccione, Francesco Lamagna and Gerardo Fatone

Vet. Sci. 2023, 10(12), 686; https://doi.org/10.3390/vetsci10120686 - 1 Dec 2023

Viewed by 1605

Abstract

Platelet concentrates (PCs) have become widely used in veterinary and human medicine. The PCs consist mainly of supraphysiological concentrations of platelets and, therefore, growth factors (GFs) which are stored within platelet α-granules. Among PCs, Platelet-Rich Plasma (PRP) is characterised by low-density fibrin. Research on the effect of PCs in cattle has surged in recent years; in particular, evidence has shown the positive use of PRP for treating reproductive problems, in vitro production of bovine embryos, sole ulcers and udder diseases. The aim of this report is to critically review, in accordance with the PRISMA guidelines, the available literature reporting clinical application in the bovine practice of PRP. Three bibliographic databases PubMed, Web of Science and Scopus were used for a broad search of “platelet concentrates” OR “PRP” OR “platelet-rich plasma” OR “PRF” OR “platelet-rich fibrin” AND “cows” OR “cattle”. From 1196 papers, only six met the inclusion criteria. Two papers described the use of PRP in mastitis, two papers in uterine dysfunction and two papers in ovarian dysfunction. PRP offered a low-cost, easily obtained therapeutic option and showed positive results for these patients. However, given the different pathologies and definitions involved, further studies are necessary to assess its full clinical potential. Full article

(This article belongs to the Special Issue Advancement in Wound Management: Regenerative Medicine, Cell Therapies, Skin Reconstruction in Small and Large Animal Practice)

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