Alpha-1 Antitrypsin Augmentation Inhibits Proteolysis of Neutrophil Membrane Voltage-Gated Proton Channel-1 in Alpha-1 Deficient Individuals
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Study Design
2.3. Neutrophil Isolation and Assays
2.4. SDS-Polyacrylamide Gel Electrophoresis and Western Blotting
2.5. Flow Cytometry Experiments
2.6. Statistical Analysis
3. Results
3.1. Cell Membrane Expression of HVCN1 in Neutrophils of AATD Is Decreased
3.2. HVCN1 Is Proteolytically Cleaved from Neutrophil Plasma Membranes in AATD
3.3. HVCN1 Is Proteolytically Cleaved by Neutrophil Elastase
3.4. AAT Augmentation Therapy Increases HVCN1 Expression on AATD Neutrophils
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Hawkins, P.; Sya, J.; Hup, N.K.; Murphy, M.P.; McElvaney, N.G.; Reeves, E.P. Alpha-1 Antitrypsin Augmentation Inhibits Proteolysis of Neutrophil Membrane Voltage-Gated Proton Channel-1 in Alpha-1 Deficient Individuals. Medicina 2021, 57, 814. https://doi.org/10.3390/medicina57080814
Hawkins P, Sya J, Hup NK, Murphy MP, McElvaney NG, Reeves EP. Alpha-1 Antitrypsin Augmentation Inhibits Proteolysis of Neutrophil Membrane Voltage-Gated Proton Channel-1 in Alpha-1 Deficient Individuals. Medicina. 2021; 57(8):814. https://doi.org/10.3390/medicina57080814
Chicago/Turabian StyleHawkins, Padraig, Julian Sya, Nee Kee Hup, Mark P. Murphy, Noel G. McElvaney, and Emer P. Reeves. 2021. "Alpha-1 Antitrypsin Augmentation Inhibits Proteolysis of Neutrophil Membrane Voltage-Gated Proton Channel-1 in Alpha-1 Deficient Individuals" Medicina 57, no. 8: 814. https://doi.org/10.3390/medicina57080814