Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analysis of Eight Signature Compounds by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry with Multiple Reaction Monitoring
2.2. The Antioxidant Activity, α-Glucosidase Inhibition Activity and Content of Total Polyphenols
3. Materials and Methods
3.1. Reagents
3.2. Plant Collection, Identification and Extraction
3.3. HPLC-ESI-MS Analysis of the Extract
3.4. Method Validation and Quantitation of Phenolic Acid and Water-Soluble Vitamins
3.5. Determination of Total Polyphenol Content
3.6. DPPH Radical Scavenging Activity
3.7. α-Glucosidase Inhibition
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the Eccoilopus formosanus are not available from the authors. |
Compound | MRM Transition (m/z) | DP (V) | CE (eV) | CXP (V) |
---|---|---|---|---|
Thiamine | 265 >122 | 62 | 22 | 18 |
Pyridoxamin | 170 > 152 | 70 | 28 | 7 |
Gallic acid | 169 > 124.8 | −29 | −23 | −27 |
Chlorogenic acid | 355 >163 | 66 | 14 | 10 |
Caffeic acid | 181 >163 | 45 | 17 | 9 |
Riboflavin | 377.2 >180.7 | 50 | 20 | 10 |
Ferulic acid | 193 > 134 | −36 | −20 | −4.5 |
Apigenin | 269 > 117 | −90 | −52 | −15 |
Compound | Retention Time (tR) | Regression Equation | R2 | Range (μg/mL) | LOQ (μg/mL) | Precision (%) | Recovery (%) |
---|---|---|---|---|---|---|---|
Thiamine | 2.32 | y = 9062.1x−2446.1 | 0.995 | 1–50 | 0.3 | 2.3 | 94.1 |
Pyridoxamin | 2.81 | y = 2191.6x−695.89 | 0.995 | 1–50 | 0.2 | 1.7 | 81.5 |
Gallic acid | 4.30 | y= 2915.1x−3240 | 0.996 | 1–50 | 1.2 | 3.5 | 90.5 |
Chlorogenic acid | 14.08 | y = 1058.3x−60.5 | 0.997 | 10–200 | 1.6 | 1.5 | 91.2 |
Caffeic acid | 15.39 | y = 5695.4−1165.5 | 0.999 | 1–50 | 0.9 | 1.4 | 86.7 |
Riboflavin | 15.66 | y =1415.8+1248.6 | 0.995 | 1–50 | 0.1 | 1.2 | 85.5 |
Ferulic acid | 18.34 | y = 934.5−2352.1 | 0.996 | 10–200 | 1.5 | 1.9 | 82.3 |
Apigenin | 23.98 | y = 3276.6x−658.1 | 0.997 | 1–50 | 0.5 | 2.9 | 92.1 |
Sample | Thiamine | Pyridoxamin | Gallic Acid | Chlorogenic Acid | Caffeic Acid | Riboflavin | Ferulic Acid | Apigenin |
---|---|---|---|---|---|---|---|---|
Extract of E. formosanus | 4.6 | 1.5 | 11.0 | 179.1 | 65.2 | 2.1 | 145.3 | 15.2 |
Samples | Yield (%) | Total Phenolic Content (mg GAE/g, DW) |
---|---|---|
30% ethanol | 37.1 ± 0.3 | 2.5 ± 0.3 |
50% ethanol | 45.5 ± 0.2 | 3.1 ± 0.1 |
70% ethanol | 40.4 ± 0.6 | 2.9 ± 0.5 |
Samples | DPPH Radical Scavenging Activity, IC50 Value (µg/mL) | α-Glucosidase Inhibition Activity, IC50 Value (µg/mL) |
---|---|---|
E. formosanus extract | 120.7 ± 0.3 | 136.9 ± 0.5 |
Thiamine | >500 | >500 |
Pyridoxamin | >500 | >500 |
Gallic acid | 21.1 ± 0.3 | 59.2± 0.6 |
Chlorogenic acid | 12.7 ± 0.5 | 9.1 ± 0.5 |
Caffeic acid | 25.3 ± 0.3 | 7.2 ± 0.2 |
Riboflavin | 155.7 ± 0.4 | >500 |
Ferulic acid | 45.2 ± 0.3 | 107.1 ± 0.9 |
Apigenin | 31.6 ± 0.9 | 79.1 ± 0.6 |
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Huang, H.-S.; Yu, H.-S.; Yen, C.-H.; Liaw, E.-T. Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus. Molecules 2020, 25, 3632. https://doi.org/10.3390/molecules25163632
Huang H-S, Yu H-S, Yen C-H, Liaw E-T. Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus. Molecules. 2020; 25(16):3632. https://doi.org/10.3390/molecules25163632
Chicago/Turabian StyleHuang, Ho-Shin, Hsu-Sheng Yu, Chia-Hung Yen, and Ean-Tun Liaw. 2020. "Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus" Molecules 25, no. 16: 3632. https://doi.org/10.3390/molecules25163632