Acetylharpagide Protects Mice from Staphylococcus Aureus-Induced Acute Lung Injury by Inhibiting NF-κB Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Acetylharpagide Protects Mice from S. aureus-Induced ALI
2.2. Acetylharpagide Protects the Pulmonary Blood-Gas Barrier in S. aureus-Induced ALI
2.3. Acetylharpagide Attenuates S. aureus-Induced Inflammation
2.4. Acetylharpagide Inhibits S. aureus-Induced Pro-Inflammatory Cytokines in Macrophages
2.5. Acetylharpagide Suppresses S. aureus-Induced NF-κB Activity
2.6. Acetylharpagide Inhibits the Interaction between Polyubiquitinated RIP1 and NEMO upon S. aureus Infection
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Animals
4.3. Western Blot
4.4. Pulmonary Vascular Permeability and Edema
4.5. Cytokines and Neutrophils in BAL Fluid
4.6. Bacterial Counts
4.7. Coimmunoprecipitation Assay
4.8. Dual Luciferase Reporter Assay
4.9. Quantitative Real-Time PCR
4.10. Extraction of Cytoplasm and Nuclear Proteins
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zhang, Z.; Wang, Y.; Shan, Y.; Yin, W. Acetylharpagide Protects Mice from Staphylococcus Aureus-Induced Acute Lung Injury by Inhibiting NF-κB Signaling Pathway. Molecules 2020, 25, 5523. https://doi.org/10.3390/molecules25235523
Zhang Z, Wang Y, Shan Y, Yin W. Acetylharpagide Protects Mice from Staphylococcus Aureus-Induced Acute Lung Injury by Inhibiting NF-κB Signaling Pathway. Molecules. 2020; 25(23):5523. https://doi.org/10.3390/molecules25235523
Chicago/Turabian StyleZhang, Zhaoxin, Yun Wang, Yating Shan, and Wu Yin. 2020. "Acetylharpagide Protects Mice from Staphylococcus Aureus-Induced Acute Lung Injury by Inhibiting NF-κB Signaling Pathway" Molecules 25, no. 23: 5523. https://doi.org/10.3390/molecules25235523