Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
Abstract
:1. Introduction
2. Results and Discussion
2.1. Castor Accession Line 337 Has Less Lupeol Content on Its Hypocotyl Compared to the Stem
2.2. RNA-Sequencing, Transcript Assembly, and Annotation
2.3. Comparative Transcriptome Enrichment Analysis between Upper Hypocotyl and First Internode of the Stem of Accession Line 337
2.4. Genes Closely Related with Lupeol Biosynthesis in Castor Epidermis
2.5. Auxin Signaling or Early Response Pathway Is Highly Enriched in the Upregulated DEGs
2.6. Auxin Treatment Enhanced Lupeol Accumulation in the Epidermis of Castor Leaves
3. Materials and Methods
3.1. Plant Materials
3.2. Cuticular Wax Analysis
3.3. Scanning Electron Microscopy (SEM)
3.4. RNA Extraction, cDNA Library Preparation, and Transcriptome Sequencing
3.5. Transcriptome Assembly, Functional Annotation, and DEG Analysis
3.6. Quantitative Reverse Transcription-PCR (qRT-PCR)
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene-ID | log2FC * | p-Value | Gene Description |
---|---|---|---|
LOC8280179 | 2.297 | 0.0002 | auxin-responsive protein IAA7 |
LOC8271341 | 1.957 | 0.0005 | auxin-induced protein AUX22 |
LOC8285710 | 2.956 | 0.0002 | indole-3-acetic acid-amido synthetase GH3.17 |
LOC107262116 | 3.171 | 0.0001 | auxin-induced protein 15A-like |
LOC107260799 | 4.896 | 0.0001 | auxin-induced protein 15A-like |
LOC107262113 | 6.189 | 0.0002 | auxin-induced protein 15A-like |
LOC107262111 | 3.754 | 0.0002 | auxin-induced protein 15A-like |
LOC107262117 | 5.028 | 0.0008 | auxin-induced protein 15A-like |
LOC107261577 | 4.484 | 0.0001 | auxin-responsive protein SAUR21-like |
LOC107261578 | 6.831 | 0.0000 | auxin-responsive protein SAUR21-like |
LOC107261581 | 7.976 | 0.0000 | auxin-responsive protein SAUR21-like |
LOC107261586 | 8.247 | 0.0000 | auxin-responsive protein SAUR21-like |
LOC107261587 | 7.936 | 0.0000 | auxin-responsive protein SAUR21-like |
LOC8283211 | 3.697 | 0.0003 | auxin-responsive protein SAUR62-like |
LOC8283214 | 7.806 | 0.0000 | auxin-responsive protein SAUR63-like |
LOC8283212 | 6.678 | 0.0000 | auxin-responsive protein SAUR63-like |
LOC8266878 | 8.459 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8266881 | 8.433 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8266894 | 8.010 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8266888 | 2.562 | 0.0003 | auxin-responsive protein SAUR68-like |
LOC8266882 | 6.531 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8266895 | 7.277 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8266875 | 6.060 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8266880 | 4.946 | 0.0004 | auxin-responsive protein SAUR68-like |
LOC8266887 | 6.710 | 0.0000 | auxin-responsive protein SAUR68-like |
LOC8283213 | 5.393 | 0.0003 | auxin-responsive protein SAUR68-like |
LOC8266876 | 6.118 | 0.0003 | auxin-responsive protein SAUR68-like |
LOC8266884 | 5.706 | 0.0008 | auxin-responsive protein SAUR68-like |
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Li, D.; Pan, C.; Lu, J.; Zaman, W.; Zhao, H.; Zhang, J.; Lü, S. Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor. Molecules 2021, 26, 2978. https://doi.org/10.3390/molecules26102978
Li D, Pan C, Lu J, Zaman W, Zhao H, Zhang J, Lü S. Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor. Molecules. 2021; 26(10):2978. https://doi.org/10.3390/molecules26102978
Chicago/Turabian StyleLi, Donghai, Cheng Pan, Jianjun Lu, Wajid Zaman, Huayan Zhao, Jixing Zhang, and Shiyou Lü. 2021. "Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor" Molecules 26, no. 10: 2978. https://doi.org/10.3390/molecules26102978