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Article

The Overexpression of YALI0B07117g Results in Enhanced Erythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica

by
Mateusz Szczepańczyk
,
Dorota A. Rzechonek
,
Adam Dobrowolski
and
Aleksandra M. Mirończuk
*
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, 51-630 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(24), 7549; https://doi.org/10.3390/molecules26247549
Submission received: 19 November 2021 / Revised: 6 December 2021 / Accepted: 8 December 2021 / Published: 13 December 2021
(This article belongs to the Topic Applied Sciences in Functional Foods)
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Abstract

The unconventional yeast Yarrowia lipolytica is used to produce erythritol from glycerol. In this study, the role of the erythrose reductase (ER) homolog YALI0B07117g in erythritol synthesis was analyzed. The deletion of the gene resulted in an increased production of mannitol (308%) and arabitol (204%) before the utilization of these polyols began. The strain overexpressing the YALI0B07117g gene was used to increase the erythritol yield from glycerol as a sole carbon source in batch cultures, resulting in a yield of 0.4 g/g. The specific consumption rate (qs) increased from 5.83 g/g/L for the WT strain to 8.49 g/g/L for the modified strain and the productivity of erythritol increased from 0.28 g/(L h) for the A101 strain to 0.41 g/(L h) for the modified strain. The application of the research may prove positive for shortening the cultivation time due to the increased rate of consumption of the substrate combined with the increased parameters of erythritol synthesis.
Keywords: Yarrowia lipolytica; erythritol; glycerol; erythrose reductase; sweetener Yarrowia lipolytica; erythritol; glycerol; erythrose reductase; sweetener

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MDPI and ACS Style

Szczepańczyk, M.; Rzechonek, D.A.; Dobrowolski, A.; Mirończuk, A.M. The Overexpression of YALI0B07117g Results in Enhanced Erythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica. Molecules 2021, 26, 7549. https://doi.org/10.3390/molecules26247549

AMA Style

Szczepańczyk M, Rzechonek DA, Dobrowolski A, Mirończuk AM. The Overexpression of YALI0B07117g Results in Enhanced Erythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica. Molecules. 2021; 26(24):7549. https://doi.org/10.3390/molecules26247549

Chicago/Turabian Style

Szczepańczyk, Mateusz, Dorota A. Rzechonek, Adam Dobrowolski, and Aleksandra M. Mirończuk. 2021. "The Overexpression of YALI0B07117g Results in Enhanced Erythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica" Molecules 26, no. 24: 7549. https://doi.org/10.3390/molecules26247549

APA Style

Szczepańczyk, M., Rzechonek, D. A., Dobrowolski, A., & Mirończuk, A. M. (2021). The Overexpression of YALI0B07117g Results in Enhanced Erythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica. Molecules, 26(24), 7549. https://doi.org/10.3390/molecules26247549

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