Metabolite Differences of Polyphenols in Different Litchi Cultivars (Litchi chinensis Sonn.) Based on Extensive Targeted Metabonomics
Abstract
:1. Introduction
2. Results
2.1. Qualitative/Quantitative Analysis of Metabolites and Quality Control Analysis
2.2. UPLC-MS/MS Analysis
2.3. Multivariate Statistical Analysis of Metabonomics
2.3.1. Principal Component Analysis (PCA)
2.3.2. Cluster Analysis
2.3.3. Orthogonal Projections to Latent Structures-Discriminant Analysis (OPLS-DA)
2.4. Screening and Identification of Different Metabolites
3. Discussion
3.1. Identification of Polyphenol Metabolites in Litchi
3.2. Differences of Polyphenol Metabolites in Different Litchi Cultivars
4. Materials and Methods
4.1. Materials and Instruments
4.2. Treatment and Method
4.3. Collection Conditions for Chromatography-Mass Spectrometry
4.4. Quality Control Samples
4.5. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Group Name | All Sig Diff | Down Regulated | Up Regulated |
---|---|---|---|
BTY vs. FZX | 44 | 37 | 7 |
FC vs. FZX | 39 | 25 | 14 |
YJQ vs. FZX | 50 | 44 | 6 |
BYL vs. FZX | 65 | 49 | 16 |
No | Cultivars | Abbreviation | Maturity Type | Harvest Time | Harvest Site | Longitude and Latitude |
---|---|---|---|---|---|---|
1 | Feizhixiao | FZX | Early-maturing | 2 June 2018 | Tianhe, Guangzhou | 23°9′13″ N, 113°9′13″ E |
2 | Baitangyin | BTY | 6 June 2018 | Tianhe, Guangzhou | 23°9′13″ N, 113°9′13″ E | |
3 | Feicui | FC | Medium-maturating | 24 June 2018 | Gaozhou, Maoming | 21°51′25″ N, 110°53′50″ E |
4 | Yujinqiu | YJQ | 28 June 2018 | Doumen, Zhuhai | 22°13′10″ N,113°15′29″ E | |
5 | Beiyuanlv | BYL | Late-maturating | 5 July 2018 | Zengcheng, Guangzhou | 23°20′30″ N,113°48′49″ E |
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Jiang, N.; Zhu, H.; Liu, W.; Fan, C.; Jin, F.; Xiang, X. Metabolite Differences of Polyphenols in Different Litchi Cultivars (Litchi chinensis Sonn.) Based on Extensive Targeted Metabonomics. Molecules 2021, 26, 1181. https://doi.org/10.3390/molecules26041181
Jiang N, Zhu H, Liu W, Fan C, Jin F, Xiang X. Metabolite Differences of Polyphenols in Different Litchi Cultivars (Litchi chinensis Sonn.) Based on Extensive Targeted Metabonomics. Molecules. 2021; 26(4):1181. https://doi.org/10.3390/molecules26041181
Chicago/Turabian StyleJiang, Nonghui, Huili Zhu, Wei Liu, Chao Fan, Feng Jin, and Xu Xiang. 2021. "Metabolite Differences of Polyphenols in Different Litchi Cultivars (Litchi chinensis Sonn.) Based on Extensive Targeted Metabonomics" Molecules 26, no. 4: 1181. https://doi.org/10.3390/molecules26041181