Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo
Abstract
:1. Introduction
2. Results
2.1. Effects of Renifolin F on Total Serum IgE and Allergen-Specific IgE Production in Mice Serum
2.2. Effects of Renifolin F on Eotaxin, IL-4, IL-5, IL-13, IL-9 and IL-6 Levels in Mice BALF
2.3. Effects of Renifolin F on Symptoms and Muc5ac in OVA-Induced Allergic Asthma Mice
2.4. Effects of Renifolin F on ILC2s Cells and Its Upstream Cytokines in the Lung
2.5. Effects of Renifolin F on the Expression of microRNA-155 in the Lung of Allergic Asthma Mice
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Renifolin F Analysis
4.2. Mice
4.3. OVA-Induced Allergic Asthma Model and Treatment
4.4. Collection and Management of Serum
4.5. Collection and Management of the Mice Bronchoalveloar Lavage Fluid (BALF)
4.6. Measurement of Cytokine Production Levels by ELISA
4.7. Assessment of Airway Hyperresponsiveness (AHR)
4.8. Histological Analysis of Lung Tissue
4.9. Flow Cytometric Analysis
4.10. RNA Isolation and microRNA-155 Expression
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Yang, Z.; Li, X.; Fu, R.; Hu, M.; Wei, Y.; Hu, X.; Tan, W.; Tong, X.; Huang, F. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo. Molecules 2022, 27, 3789. https://doi.org/10.3390/molecules27123789
Yang Z, Li X, Fu R, Hu M, Wei Y, Hu X, Tan W, Tong X, Huang F. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo. Molecules. 2022; 27(12):3789. https://doi.org/10.3390/molecules27123789
Chicago/Turabian StyleYang, Zhuya, Xiaohong Li, Rongbing Fu, Min Hu, Yijie Wei, Xuhong Hu, Wenhong Tan, Xiaoyun Tong, and Feng Huang. 2022. "Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo" Molecules 27, no. 12: 3789. https://doi.org/10.3390/molecules27123789