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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

D-Limonene Inhibits Pichia kluyveri Y-11519 in Sichuan Pickles by Disrupting Metabolism

by
Chaoyi Zeng
1,2,3,*,
Yue Sun
1,3,
Haoran Lin
1,3,
Ziyu Li
1,3,
Qing Zhang
1,3,
Ting Cai
1,3,
Wenliang Xiang
1,3,
Jie Tang
1,2,3 and
Patchanee Yasurin
2
1
College of Food and Bioengineering, Xihua University, Chengdu 610039, China
2
Department of Food Biotechnology, Faculty of Biotechnology, Assumption University, Bangkok 10240, Thailand
3
Food Microbiology Key Laboratory of Sichuan Province, Xihua University, Chengdu 610039, China
*
Author to whom correspondence should be addressed.
Molecules 2024, 29(15), 3561; https://doi.org/10.3390/molecules29153561 (registering DOI)
Submission received: 4 June 2024 / Revised: 13 July 2024 / Accepted: 26 July 2024 / Published: 28 July 2024
Versions Notes

Abstract

The Pichia kluyveri, a proliferation commonly found in Sichuan pickles (SCPs), can accelerate the growth and reproduction of spoilage bacteria, causing off-odor development and decay. Although D-limonene, a common natural preservative, effectively restricts P. kluyveri, its inhibitory mechanism remains unclear. This study aimed to elucidate this molecular mechanism by investigating the impact on basic P. kluyveri metabolism. The findings revealed that D-limonene inhibited P. kluyveri growth and disrupted the transcription of the genes responsible for encoding the enzymes involved in cell wall and membrane synthesis, oxidative phosphorylation, glycolysis, and the tricarboxylic acid (TCA) cycle pathway. The results indicated that these events disrupted crucial metabolism such as cell wall and membrane integrity, adenosine triphosphate (ATP) synthesis, and reactive oxygen species (ROS) balance. These insights provided a comprehensive understanding of the inhibitory effect of D-limonene on the growth and reproduction of P. kluyveri while highlighting its potential application in the SCP industry.
Keywords: Pichia kluyveri; D-limonene; cell wall and membrane synthesis; oxidative stress; glycolysis and tricarboxylic acid cycle Pichia kluyveri; D-limonene; cell wall and membrane synthesis; oxidative stress; glycolysis and tricarboxylic acid cycle

Share and Cite

MDPI and ACS Style

Zeng, C.; Sun, Y.; Lin, H.; Li, Z.; Zhang, Q.; Cai, T.; Xiang, W.; Tang, J.; Yasurin, P. D-Limonene Inhibits Pichia kluyveri Y-11519 in Sichuan Pickles by Disrupting Metabolism. Molecules 2024, 29, 3561. https://doi.org/10.3390/molecules29153561

AMA Style

Zeng C, Sun Y, Lin H, Li Z, Zhang Q, Cai T, Xiang W, Tang J, Yasurin P. D-Limonene Inhibits Pichia kluyveri Y-11519 in Sichuan Pickles by Disrupting Metabolism. Molecules. 2024; 29(15):3561. https://doi.org/10.3390/molecules29153561

Chicago/Turabian Style

Zeng, Chaoyi, Yue Sun, Haoran Lin, Ziyu Li, Qing Zhang, Ting Cai, Wenliang Xiang, Jie Tang, and Patchanee Yasurin. 2024. "D-Limonene Inhibits Pichia kluyveri Y-11519 in Sichuan Pickles by Disrupting Metabolism" Molecules 29, no. 15: 3561. https://doi.org/10.3390/molecules29153561

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