Location, Location, Location: Establishing Design Principles for New Antibacterials from Ferric Siderophore Transport Systems
Abstract
:1. Antibiotic Development and Microbial Iron Assimilation
2. Bacterial Infrastructure of Iron Siderophore Transport
2.1. Acinetobacter baumannii
2.1.1. Preacinetobactin and Acinetobactin
2.1.2. Fimsbactins
2.2. Escherichia coli
2.2.1. Ferrichrome and Related Hydroxamates
2.2.2. Enterobactin and Related Catecholates
2.3. Pseudomonas aeruginosa
2.3.1. Pyoverdine and Pyochelin
2.3.2. Enterobactin
3. Siderophore Conjugate Studies
3.1. Albomycin and Hydroxamate Siderophore Conjugates
3.2. Fimsbactin conjugates
3.3. Enterobactin Conjugates and Derivatives
3.4. Catecholate Siderophore Conjugates
3.5. Pyochelin conjugates
4. Cefiderocol
5. Conclusions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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A. baumannii | E. coli | P. aeruginosa | ||||
---|---|---|---|---|---|---|
(Pre)acinetobactin (Pre)Acb | Ferrichrome Fch | Enterobactin Ent | Catecholate Siderophores | Pyochelin Pch | Enterobactin Ent | |
Outer membrane (TBDT) | BauA | FhuA | FepA IroN | Fiu CirA | FptA | PfeA PirA |
Periplasm (PBP) | BauB | FhuD | FepB | Unknown ? | FepB ? | FepB ? |
Inner membrane (ABC) | BauCDE | FhuCB | FepDGC | Unknown ? | FptX FepDGC PchHI | FepDGC ? |
Cytoplasm | BauF * | FhuF * | Fes # YgjH * | Unknown ? | Unknown ? | Unknown ? |
Entry | Strain | W6 | S1a-W6 | S1b-W6 | S2a-W6 | S2b-W6 |
---|---|---|---|---|---|---|
1 a | A. baumannii ATCC 17961 | 15 b | - | - | 18 | 20 |
2 | E. coli X580 | 31 c | 34 | 32 | 21 | 27 |
3 | P. aeruginosa K799/WT | 21 d | 27 | 18 | 14 | 19 |
4 | P. aeruginosa K799/61 | 24 d | 31 | 32 | 0 | 19 |
Entry | Strain | W3 | S2a-W3 |
---|---|---|---|
1 | A. baumannii ATCC 17961 | >128 | 0.125 |
2 | E. coli ATCC 25922 | 2 | 8 |
3 | P. aeruginosa ATCC 27853 | >128 | >128 |
Entry | Strain | W9 | S2a-W9 | S4-W9 |
---|---|---|---|---|
1 | A. baumannii ATCC 17961 | >100 | 0.4 | 0.2 |
2 | A. baumannii ATCC 17978 | - | - | 0.8 |
3 | A. baumannii BAA 1710 | >100 | 0.8 | - |
4 | A. baumannii BAA 1793 | >100 | 0.8 | - |
5 | A. baumannii BAA 1797 | >100 | 0.8 | - |
6 | A. baumannii BAA 1800 | >100 | 0.8 | - |
7 | A. baumannii ARC 3484 | >100 | 0.4 | 3 |
8 | A. baumannii ARC 3486 | >100 | 0.4 | 3 |
9 | A. baumannii ARC 5079 | >100 | 0.8 | 12.5 |
10 | A. baumannii ARC 5081 | >100 | 0.4 | 12.5 |
11 | A. baumannii ATCC 19606 | - | 0.8 | - |
12 | E. coli DCO | >100 | >100 | >50 |
13 | P. aeruginosa PAO1 | >100 | >100 | - |
14 | P. aeruginosa KW799/WT | >50 | - | >50 |
15 | P. aeruginosa ARC 3502 | >50 | - | >50 |
Entry | Strain | W1 | W2 | S3a-W1 | S3a-W2 | S4-W1 | S4-W2 | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
+Fe | −Fe | +Fe | −Fe | +Fe | −Fe | +Fe | −Fe | +Fe | −Fe | +Fe | −Fe | ||
1 | P. aeruginosa PAO1 | n.a. | n.a. | 10 | 10 | 10 | 10 | 50 | 0.39 | 50 | 0.39 | ||
2 | E. coli ATCC 25922 | 16.7 | 12.5 | 4.17 | 4.17 | 10 | 0.1 | 10 | 0.1 | 150 | 1.56 | 100 | 6.15 |
3 | E. coli UTI89 | 10 | 10 | 10 | 10 | 1 | 0.1 | 10 | 0.1 | - | - | - | - |
4 | E. coli CFT073 | 10 | 10 | 10 | 10 | 0.1 | 0.01 | 0.1 | 0.01 | - | - | - | - |
5 | E. coli H9049 | 10 | 10 | 10 | 10 | 10 | 0.1 | 10 | 0.1 | - | - | - | - |
6 | E. coli 35401 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | - | - | - | - |
7 | E. coli 43895 | 10 | 10 | 10 | 10 | 10 | 1 | 10 | 1 | - | - | - | - |
8 | E. coli K-12 | 10 | 10 | 10 | 10 | 10 | 0.1 | 10 | 0.1 | - | - | - | - |
9 | E. coli K-12 fepA- | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | - | - | - | - |
10 | E. coli K-12 fepC- | 10 | 10 | 10 | 10 | 1 | 1 | 10 | 0.1 | - | - | - | - |
11 | E. coli K-12 fes- | 10 | 10 | 10 | 10 | 1 | 0.1 | 1 | 0.1 | - | - | - | - |
12 | P. aeruginosa K799/WT | n.a. | n.a. | - | - | - | - | 33 | 0.05 | 25 | 0.05 | ||
13 | P. aeruginosa K799/61 | 0.52 | 0.78 | 0.46 | 0.39 | - | - | - | - | 12.5 | 0.067 | 12.5 | 0.083 |
14 | P. aeruginosa Pa4 | n.a. | n.a. | - | - | - | - | 25 | 0.39 | 25 | 0.21 | ||
15 | P. aeruginosa Pa6 | n.a. | n.a. | - | - | - | - | n.a. | n.a. | n.a. | n.a. |
Entry | Strain | W6 (Cipro) | S3b-W6 | S3c-W6 * | |
---|---|---|---|---|---|
+Fe | −Fe | ||||
1 | E. coli K-12 | 0.1 | n.a. | n.a. | n.a. |
2 | E. coli B | 0.1 | n.a. | n.a. | n.a. |
3 | E. coli UTI89 | 0.1 | n.a. | 0.1 | 0.1 |
4 | E. coli CFT073 | 0.1 | n.a. | 1 | 0.1 |
5 | E. coli CFT073 fepA- | - | - | 0.1 | |
6 | E. coli CFT073 iroN- | - | - | 0.1 | |
7 | E. coli CFT073 fepA- iroN- | - | - | n.a. | |
8 | E. coli CFT073 fepC- | - | - | n.a. | |
9 | E. coli CFT073 fepDG- | - | - | n.a. | |
10 | E. coli CFT073 fes- | - | - | 1 | |
11 | E. coli CFT073 iroD- | - | - | n.a. |
Entry | Strain | W1 | W5 | S5-W1 | S6-W1 | S7-W1 | S8-W1 | S9-W1 | S10-W4 | S11-W4 | S12-W1 | S13-W1 | S13-W2 | S13-W5 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | E. coli ATCC 25922 | 6.26 | 12.5 | 0.78 | 0.4 | 0.2 | 0.4 | 0.1 | n.a. | 100 | 50 | 6.25 | 3.12 | 1.56 |
2 | P. aeruginosa ATCC 27853 | n.a. | 100 | 0.4 | 0.4 | 0.78 | 0.4 | 0.2 | 3.12 | 25 | 6.25 | 0.78 | 6.25 | 50 |
3 | P. aeruginosa SG 137 | n.a. | 100 | <0.05 | <0.05 | 0.05 | <0.05 | 0.01 | <0.05 | 12.5 | 0.4 | 0.2 | 0.78 | 50 |
Entry | Strain | W10 | S14a-W10 | S14b-W10 |
---|---|---|---|---|
1 | A. baumannii ATCC 17961 | n.a. | n.a. | 0.8 |
2 | A. baumannii ATCC BAA 1793 | n.a. | n.a. | 0.8–0.16 |
3 | A. baumannii ATCC BAA 1797 | n.a. | n.a. | 6.25 |
4 | A. baumannii ATCC BAA 1800 | n.a. | n.a. | 0.8 |
5 | E. coli DC0 | n.a. | n.a. | <0.025 |
6 | P. aeruginosa KW799/WT | n.a. | n.a. | 0.2–0.4 |
Entry | Strain | W6 | W7 | W8 | S15f-W6 | S15f-W7 | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
+Fe | −Fe | +Fe | −Fe | +Fe | −Fe | +Fe | −Fe | +Fe | −Fe | ||
1 | P. aeruginosa PAO1 | 0.060 | 0.040 | 0.200 | 0.110 | 0.350 | 0.200 | 0.700 | 0.600 | 1.000 | n.a. |
2 | P. aeruginosa PAD07 (pvd- pch-) | 0.045 | 0.060 | 0.190 | 0.200 | 0.360 | 0.350 | 0.600 | 0.700 | 1.000 | 1.000 |
3 | P. aeruginosa PAD14 (tonB-) | 0.040 | 0.035 | 0.120 | 0.120 | 0.210 | 0.180 | 0.200 | 0.170 | 0.550 | 0.450 |
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Luo, V.C.; Peczuh, M.W. Location, Location, Location: Establishing Design Principles for New Antibacterials from Ferric Siderophore Transport Systems. Molecules 2024, 29, 3889. https://doi.org/10.3390/molecules29163889
Luo VC, Peczuh MW. Location, Location, Location: Establishing Design Principles for New Antibacterials from Ferric Siderophore Transport Systems. Molecules. 2024; 29(16):3889. https://doi.org/10.3390/molecules29163889
Chicago/Turabian StyleLuo, Vivien Canran, and Mark W. Peczuh. 2024. "Location, Location, Location: Establishing Design Principles for New Antibacterials from Ferric Siderophore Transport Systems" Molecules 29, no. 16: 3889. https://doi.org/10.3390/molecules29163889