Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Selection of Immobilization Conditions
2.2. Stability of Immobilized Lipase
2.3. Characterization of Immobilized Enzyme During Repeated Use
2.4. Immobilized Enzyme Catalyzed Glycerolysis for DAG Preparation
3. Materials and Methods
3.1. Materials
3.2. Pretreatment of Resin
3.3. Lipase Immobilization
3.4. Determination of Enzyme Activity
3.5. Protein Content of Lipase
3.6. Analysis of the Acylglycerol Compositions
3.7. Isomer Analysis
3.8. Characterization of the Immobilized Lipase
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CN-TL | TL-PEGDGE-LX | |||||
---|---|---|---|---|---|---|
sn-1,2 DAG | sn-1,3 DAG | sn-1,3 DAG/sn-1,2 DAG | sn-1,2 DAG | sn-1,3 DAG | sn-1,3 DAG/sn-1,2 DAG | |
Olive Oil | 1.75 ± 0.12 | 8.25 ± 0.12 | 4.72 ± 0.25 a | 1.96 ± 0.12 | 8.04 ± 0.12 | 4.20 ± 0.23 b |
Canola Oil | 2.72 ± 0.13 | 7.28 ± 0.13 | 2.69 ± 0.26 a | 2.77 ± 0.18 | 7.23 ± 0.18 | 2.62 ± 0.36 a |
Peanut Oil | 2.34 ± 0.09 | 7.66 ± 0.09 | 3.34 ± 0.19 a | 2.33 ± 0.14 | 7.67 ± 0.14 | 3.30 ± 0.26 a |
Soybean oil | 2.21 ± 0.15 | 7.79 ± 0.15 | 3.57 ± 0.28 a | 2.34 ± 0.23 | 7.66 ± 0.23 | 3.36 ± 0.44 a |
Corn Oil | 2.09 ± 0.08 | 7.91 ± 0.08 | 3.78 ± 0.18 a | 2.18 ± 0.14 | 7.82 ± 0.14 | 3.60 ± 0.29 a |
Palm Oil | 2.43 ± 0.15 | 7.57 ± 0.15 | 3.11 ± 0.21 a | 2.51 ± 0.11 | 7.49 ± 0.11 | 2.98 ± 0.16 a |
Almond Oil | 2.67 ± 0.09 | 7.33 ± 0.09 | 2.74 ± 0.28 a | 2.69 ± 0.23 | 7.31 ± 0.23 | 2.71 ± 0.44 a |
Sample | Olive Oil | Canola Oil | Peanut Oil | Corn Oil | Soybean Oil | Palm Oil | Almond Oil |
---|---|---|---|---|---|---|---|
Initial DAG | 1.98 ± 0.11 | 2.01 ± 0.05 | 1.40 ± 0.01 | 2.38 ± 0.06 | 1.57 ± 0.09 | 2.95 ± 0.05 | 1.78 ± 0.11 |
DAG | 38.98 ± 0.35 | 32.24 ± 0.88 | 39.76 ± 0.70 | 41.65 ± 0.13 | 34.57 ± 0.89 | 36.18 ± 1.07 | 37.31 ± 0.35 |
TAG Conversion | 56.45 ± 0.86 | 47.97 ± 0.65 | 60.87 ± 1.12 | 62.09 ± 0.97 | 52.43 ± 0.54 | 55.86 ± 0.77 | 56.01 ± 0.86 |
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Xie, R.; Lee, Y.-Y.; Xie, P.; Tan, C.-P.; Wang, Y.; Zhang, Z. Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation. Molecules 2024, 29, 4141. https://doi.org/10.3390/molecules29174141
Xie R, Lee Y-Y, Xie P, Tan C-P, Wang Y, Zhang Z. Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation. Molecules. 2024; 29(17):4141. https://doi.org/10.3390/molecules29174141
Chicago/Turabian StyleXie, Rui, Yee-Ying Lee, Pengkai Xie, Chin-Ping Tan, Yong Wang, and Zhen Zhang. 2024. "Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation" Molecules 29, no. 17: 4141. https://doi.org/10.3390/molecules29174141