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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Functional Identification of the Terpene Synthase Family Involved in Biosynthesis in Paeonia lactiflora

by
Yufeng Zhao
1,
Guanghong Cui
2,
Jian Wang
2,
Ying Ma
2,
Yang Han
2,
Ping Su
2,
Juan Guo
2,
Jiyu Zhang
1,* and
Luqi Huang
1,2,*
1
State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
2
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16 Dongzhimennei Nanxiao Street, Dongcheng District, Beijing 100700, China
*
Authors to whom correspondence should be addressed.
Molecules 2024, 29(19), 4662; https://doi.org/10.3390/molecules29194662
Submission received: 21 August 2024 / Revised: 14 September 2024 / Accepted: 28 September 2024 / Published: 30 September 2024
Versions Notes

Abstract

The root of Paeonia lactiflora pall. is a significant component of traditional Chinese medicine, with terpenoids and their glycosides, such as paeoniflorins, serving as key active ingredients known for their anti-inflammatory, hepatoprotective, and analgesic properties. By generating a transcriptome and functionally characterizing 32 terpene synthases (TPSs) from P. lactiflora, we successfully constructed 24 pESC-Trp-PlTPS expression vectors. Through expression in Saccharomyces cerevisiae engineered strains, we identified four mono-TPSs and five sesqui-TPSs that produce 18 compounds, including eight monoterpenes and ten sesquiterpenes in vitro. This includes a bifunctional enzyme (PlTPS22). Additionally, PlTPS21 was characterized as a pinene synthase with α-pinene as its main product. The expression pattern of PlTPS21 aligns closely with the accumulation patterns of paeoniflorins and α-pinene in the plant, suggesting that PlTPS21 is a key enzyme in the biosynthesis of paeoniflorin.
Keywords: Paeonia lactiflora; ·paeoniflorin; ·terpene synthases; ·monoterpene; sesquiterpene Paeonia lactiflora; ·paeoniflorin; ·terpene synthases; ·monoterpene; sesquiterpene

Share and Cite

MDPI and ACS Style

Zhao, Y.; Cui, G.; Wang, J.; Ma, Y.; Han, Y.; Su, P.; Guo, J.; Zhang, J.; Huang, L. Functional Identification of the Terpene Synthase Family Involved in Biosynthesis in Paeonia lactiflora. Molecules 2024, 29, 4662. https://doi.org/10.3390/molecules29194662

AMA Style

Zhao Y, Cui G, Wang J, Ma Y, Han Y, Su P, Guo J, Zhang J, Huang L. Functional Identification of the Terpene Synthase Family Involved in Biosynthesis in Paeonia lactiflora. Molecules. 2024; 29(19):4662. https://doi.org/10.3390/molecules29194662

Chicago/Turabian Style

Zhao, Yufeng, Guanghong Cui, Jian Wang, Ying Ma, Yang Han, Ping Su, Juan Guo, Jiyu Zhang, and Luqi Huang. 2024. "Functional Identification of the Terpene Synthase Family Involved in Biosynthesis in Paeonia lactiflora" Molecules 29, no. 19: 4662. https://doi.org/10.3390/molecules29194662

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