The Effect of Fermentation on the Chemical Constituents of Gastrodia Tuber Hallimasch Powder (GTHP) Estimated by UHPLC-Q-Orbitrap HRMS and HPLC
Abstract
:1. Introduction
2. Results
2.1. Results of UHPLC-Q-Orbitrap HRMS Analysis
2.1.1. Flavonoid Compounds of GTHP
2.1.2. Organic Acids and Nucleoside Compounds in GTHP
2.1.3. Terpenoid Compounds in GTHP
2.2. Orthogonal Projections to Latent Structures Discriminant Analysis of Pre- and Post-Fermented GTHP
2.3. Establishment of Fingerprinting and Multicomponent Content Determination of GTHP
2.3.1. Establishment of Fingerprints for GTHP
2.3.2. Linear Investigation Results
2.3.3. Methodological Investigations
2.3.4. Content Analysis of GTHP Samples before and after Fermentation
3. Discussion
4. Materials and Methods
4.1. Materials
4.1.1. Experimental
4.1.2. Experimental Reagents and Medicinal Materials
4.2. Methods
4.2.1. Preparation of HRMS Test Solution
4.2.2. Preparation of HPLC Test Solutions
4.2.3. Preparation of Standard Solutions and Standard Curves for 10 Chemical Components
4.3. Analysis Conditions
4.3.1. UHPLC and Mass Spectrometry Conditions
4.3.2. HPLC Fingerprinting and Multicomponent Content Determination Conditions
4.4. Investigation of Linear Relationship
4.4.1. Precision Test
4.4.2. Stability Test
4.4.3. Repeatability Test
4.4.4. Sample Addition Recovery Test
4.4.5. LOD and LOQ Tests
4.5. Data Analysis and Processing
4.5.1. Data Processing UHPLC-Q-Orbitrap HRMS Chromatograms
4.5.2. Processing of GTHP Sample Content Determination Data
4.5.3. SPSS Software Processing of Pre- and Post-Fermentation GTHP Data
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Serial Number | Sort | Compound | Retention Time | Molecular Formula | Ion Mode | Calculated Value (m/z) | Measured Value (m/z) | Error Value/×10−6 | Fragmentation | Identification Method | FJH | FJQ |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Alkaloid | Choline | 1.29 | C5H13NO | [M + H]+ | 104.10699 | 104.10683 | −1.54 | 60.08076, 58.06512 | Database | + | + |
2 | Alkaloid | Trigonelline | 1.38 | C7H7NO2 | [M + H]+ | 138.05496 | 138.0549 | −0.40 | 110.05998, 94.06508 | Database | + | + |
3 | Nucleoside | Cytidine * | 1.41 | C9H13N3O5 | [M + H]+ | 244.09279 | 244.09276 | −0.12 | 112.05054 | Database, literature [14] | + | + |
4 | Nucleoside | Cytosine | 1.44 | C4H5N3O | [M + H]+ | 112.05054 | 112.05040 | −1.24 | 95.02391, 94.03985, 69.04473 | Database, literature [14] | + | + |
5 | Alkaloid | Betaine * | 1.88 | C5H11NO2 | [M + H]+ | 118.08626 | 118.08625 | −0.08 | 58.06511, 59.07307 | Database | + | + |
6 | Vitamin | Nicotinic acid * | 1.94 | C6H5NO2 | [M + H]+ | 124.03931 | 124.03925 | −0.44 | 80.04941, 96.04428, 78.03378 | Database | + | + |
7 | Nucleoside | 2,6-Dihydroxypurine * | 1.99 | C5H4N4O2 | [M − H]− | 151.02615 | 151.02625 | 0.66 | 108.02042 | Database, literature [15] | + | + |
8 | Nucleoside | Uridine * | 2.00 | C9H12N2O6 | [M + H]+ | 245.07681 | 245.07684 | 0.12 | 113.03448 | Database, literature [14] | + | + |
9 | Other | Fumaric acid | 2.03 | C4H4O4 | [M − H]− | 115.00368 | 115.00382 | 1.20 | 71.01396 | Database | + | − |
10 | Other | 2-Furoic acid | 2.09 | C5H4O3 | [M − H]− | 111.00877 | 111.00905 | 2.55 | 67.01904 | Database | + | + |
11 | Other | 2-Deoxypentose | 2.20 | C5H10O4 | [M − H]− | 133.05063 | 133.05081 | 1.34 | 115.00375, 71.01393 | Database | + | − |
12 | Nucleoside | Adenosine * | 2.44 | C10H13N5O4 | [M + H]+ | 268.10403 | 268.10406 | 0.11 | 136.06174, 119.03510 | Database, literature [14,16] | + | + |
13 | Nucleoside | Guanine | 2.49 | C5H5N5O | [M + H]+ | 152.05669 | 152.05663 | −0.37 | 135.03009, 128.04570, 110.03478 | Database | + | + |
14 | Nucleoside | Guanosine * | 2.60 | C10H13N5O5 | [M + H]+ | 284.09894 | 284.09885 | −0.32 | 152.05670 | Database, literature [14] | + | + |
15 | Nucleoside | Uracil * | 2.64 | C4H4N2O2 | [M + H]+ | 113.03455 | 113.03448 | −0.62 | 96.00786 | Database, literature [14] | + | + |
16 | Vitamin | Ascorbic acid * | 3.30 | C6H8O6 | [M − H]− | 175.02481 | 175.0253 | 2.79 | 115.00316, 71.01390 | Database | + | − |
17 | Furfurals | 5-Methyl-2-furaldehyde * | 4.63 | C6H6O2 | [M + H]+ | 111.04406 | 111.04408 | 0.22 | 83.04917, 55.05423 | Database | + | − |
18 | Other | Indoline | 4.81 | C8H9N | [M + H]+ | 120.08078 | 120.08072 | −0.47 | 103.05418, 93.06983 | Database | + | + |
19 | Furfurals | 5-hydroxymethylfurfural * | 4.91 | C6H6O3 | [M + H]+ | 127.03898 | 127.03900 | 0.23 | 109.02830 | Database, literature [17] | + | − |
20 | Amino acids | D-phenylalanine * | 5.04 | C9H11NO2 | [M − H]− | 164.07170 | 164.07182 | 0.73 | 147.04529 | Database | + | + |
21 | Amino acids | 3-Hydroxy-3-methylglutaricacid | 5.18 | C6H10O5 | [M − H]− | 161.04561 | 161.04591 | 1.86 | 59.01392, 99.04560 | Database | + | + |
22 | Organic acids | Methylsuccinic acid | 5.44 | C5H8O4 | [M − H]− | 131.03498 | 131.03531 | 2.52 | 87.04517 | Database | + | + |
23 | Other | Porphobilinogen | 5.59 | C10H14N2O4 | [M − H]− | 225.08808 | 225.08815 | 0.31 | 71.01408, 59.01390 | Database | + | + |
24 | Organic acids | Pantothenic acid | 5.78 | C9H17NO5 | [M + H]+ | 220.11795 | 220.11795 | 0.00 | 202.10733, 184.09677 | Database | + | + |
25 | Organic acids | 3,4-Dihydroxyphenylacetic acid | 5.81 | C8H8O4 | [M − H]− | 167.03498 | 167.03497 | −0.06 | 123.04532 | Database | + | + |
26 | Organic acids | 3-Hydroxy-3-methylbutanoic acid | 6.46 | C5H10O3 | [M − H]− | 117.05572 | 117.05588 | 1.37 | 115.04015 | Database | + | + |
27 | Other | DL-Mandelic acid | 6.81 | C8H8O3 | [M − H]− | 151.04007 | 151.04005 | −0.12 | 107.05027 | Database | + | − |
28 | Organic acids | 5-Hydroxyindole-3-acetic acid | 6.89 | C10H9NO3 | [M + H]+ | 192.06552 | 192.06572 | 1.04 | 146.06006, 147.06815 | Database | + | − |
29 | Other | Levetiracetam * | 7.44 | C8H14N2O2 | [M + H]+ | 171.11280 | 171.11287 | 0.41 | 126.09128, 89.07086 | Database | + | + |
30 | Other | Salicylic acid | 7.73 | C7H6O3 | [M − H]− | 137.02442 | 137.02446 | 0.29 | 93.03464 | Database | + | + |
31 | Other | 2-Isopropylmalic acid | 7.91 | C7H12O5 | [M − H]− | 175.06120 | 175.06129 | 0.51 | 115.04014, 85.06591 | Database | + | + |
32 | Organic acids | Terephthalic acid | 8.56 | C8H6O4 | [M − H]− | 165.01930 | 165.01939 | 0.55 | 121.02962 | Database | + | + |
33 | Other | L-Iditol | 8.79 | C6H14O6 | [M − H]− | 181.07176 | 181.07173 | −0.17 | 71.01383, 101.02444, 89.02440 | Database | + | + |
34 | Organic acids | benzoic acid * | 9.18 | C7H6O2 | [M − H]− | 121.02950 | 121.02961 | 0.91 | 93.03453 | Database | + | + |
35 | flavonoid | Daidzin * | 9.86 | C21H20O9 | [M − H]− | 415.10346 | 415.10333 | −0.30 | 253.05060 | Database | + | + |
36 | flavonoid | Glycitin | 10.09 | C22H22O10 | [M + H]+ | 447.12857 | 447.12881 | 0.53 | 285.07568 | Database | + | − |
37 | Amide | Phenacetin * | 10.33 | C10H13NO2 | [M + H]+ | 180.10191 | 180.10208 | 0.94 | 162.09142 | Database | + | − |
38 | flavonoid | Puerarin | 10.67 | C21H20O9 | [M − H]− | 415.10345 | 415.10315 | −0.74 | 267.07166 | Database | + | − |
39 | Organic acids | Mesaconicacid | 10.78 | C5H6O4 | [M − H]− | 129.01933 | 129.01918 | −1.18 | 57.03464 | Database | + | + |
40 | flavonoid | Genistin * | 11.23 | C21H20O10 | [M − H]− | 431.09837 | 431.09869 | 0.74 | 268.03806 | Database | + | + |
41 | Other | Azelaic acid | 12.03 | C9H16O4 | [M − H]− | 187.09758 | 187.09756 | −0.12 | 125.09720, 97.06583 | Database | + | + |
42 | flavonoid | Daidzein * | 12.48 | C15H10O4 | [M + H]+ | 255.06519 | 255.06508 | −0.43 | 227.07021, 199.07533, 137.02328, 91.05417 | Database, literature [18] | + | + |
43 | flavonoid | Chrysin | 12.54 | C15H10O4 | [M − H]− | 253.05063 | 253.05075 | 0.47 | 209.06131 | Database | + | + |
44 | flavonoid | Glycitein * | 12.68 | C16H12O5 | [M − H]− | 283.06119 | 283.06122 | 0.08 | 268.03796 | Database | + | + |
45 | flavonoid | 4’,7-Dihydroxyflavanone | 12.70 | C15H12O4 | [M + H]+ | 257.08084 | 257.08072 | −0.47 | 91.05438, 81.03360 | Database | + | + |
46 | flavonoid | Genistein * | 13.43 | C15H10O5 | [M + H]+ | 271.06009 | 271.06030 | 0.74 | 253.04997, 243.06535, 215.07024, 197.05974, 169.06479, 153.01862 | Database, literature [19] | + | + |
47 | flavonoid | Naringenin * | 13.48 | C15H12O5 | [M − H]− | 271.06120 | 271.06152 | 1.18 | 151.00377, 119.05029, 107.01385 | Database | + | + |
48 | flavonoid | Fisetin | 13.49 | C15H10O6 | [M − H]− | 285.04046 | 285.04071 | 0.88 | 135.00868 | Database | + | + |
49 | flavonoid | Kaempferide * | 13.52 | C16H12O6 | [M − H]− | 299.05611 | 299.05618 | 0.23 | 285.09622 | Database | + | + |
50 | Other | Cycluron | 14.12 | C11H22N2O | [M + H]+ | 199.18049 | 199.18042 | −0.35 | 72.04436 | Database | + | + |
51 | Terpenoids | Armillarinin | 14.23 | C24H29O7Cl | [M + H]+ | 465.16746 | 465.16757 | 0.24 | 199.01570 | Database, literature [20] | + | − |
52 | Terpenoids | Soyasaponin I | 14.45 | C48H78O18 | [M + H]+ | 943.52609 | 943.526 | −0.10 | 441.37283, 599.39441, 797.46838 | Database | + | + |
53 | Sphingolipid | 2-Amino-1,3,4-octadecanetriol | 14.70 | C18H39NO3 | [M + H]+ | 318.30027 | 318.30026 | −0.03 | 300.29114 | Database | + | + |
54 | Terpenoids | Armillarilin | 14.89 | C24H30O7 | [M + H]+ | 431.20643 | 431.20645 | 0.05 | 165.05458 | Database, literature [20] | + | − |
55 | Steroid | Estriol | 15.4 | C18H24O3 | [M + H]+ | 289.17982 | 289.17978 | −0.14 | 159.08081 | Database | + | − |
56 | Terpenoids | Armillarin | 15.83 | C24H30O6 | [M + H]+ | 415.21152 | 415.21149 | −0.07 | 165.05475 | Database, literature [21] | + | + |
57 | Terpenoids | Dehydroeburicoic acid | 16.23 | C31H48O3 | [M + H]+ | 469.36762 | 469.36755 | −0.15 | 451.35825 | Database | + | − |
58 | Terpenoids | Armillaribin | 16.72 | C24H28O5 | [M + H]+ | 397.20095 | 397.20105 | 0.25 | 215.14302, 165.05461 | Database, literature [22] | + | + |
59 | Other | Piptamine | 16.81 | C23H41N | [M + H]+ | 332.33118 | 332.33112 | −0.18 | 240.26860, 91.05413 | Database | + | + |
60 | flavonoid | ar-Turmerone | 16.87 | C15H20O | [M + H]+ | 217.15869 | 217.15857 | −0.55 | 91.05415 | Database | + | − |
61 | Terpenoids | Armillaricin | 16.92 | C24H27O5Cl | [M + H]+ | 431.16198 | 431.16202 | 0.09 | 215.14302, 199.01567, 187.14812 | Database, literature [22] | + | − |
62 | Other | Cetrimonium | 16.97 | C19H41N | [M + H]+ | 284.33118 | 284.33118 | 0.01 | 60.08073 | Database | + | − |
63 | Other | Coriolic acid | 17.22 | C18H32O3 | [M − H]− | 295.22787 | 295.22809 | 0.75 | 277.21747, 195.13911 | Database | + | + |
64 | Terpenoids | Melleolide | 17.24 | C23H28O6 | [M + H]+ | 401.19587 | 401.1958 | −0.17 | 233.15363 | Database, literature [22] | + | − |
65 | Other | Phthalic anhydride | 18.29 | C8H4O3 | [M + H]+ | 149.02332 | 149.02328 | −0.27 | 121.02829 | Database | + | − |
66 | Esters | Dioctyl phthalate | 19.81 | C24H38O4 | [M + H]+ | 391.28427 | 391.28436 | 0.19 | 71.08540 | Database | + | + |
67 | Amide | Linoleamide | 19.96 | C18H33NO | [M + H]+ | 280.26349 | 280.26355 | 0.21 | 263.23700, 245.22643 | Database, literature [23] | + | + |
68 | Sphingolipid | D-Sphingosine | 20.09 | C18H37NO2 | [M + H]+ | 300.28971 | 300.28976 | 0.17 | 282.27960, 283.26321 | Database | + | + |
69 | Esters | 1-Linoleoyl glycerol | 20.51 | C21H38O4 | [M + H]+ | 355.28429 | 355.284 | −0.81 | 91.05740 | Database | + | + |
70 | Amide | Palmitamide | 21.14 | C16H33NO | [M + H]+ | 256.26349 | 256.26324 | −0.98 | 74.06001, 69.06985, 57.06992, 55.05424 | Database | + | + |
71 | Organic acids | Linoleic acid | 21.47 | C18H32O2 | [M − H]− | 279.23295 | 279.23306 | 0.38 | 261.22192 | Database, literature [24] | + | + |
72 | Amide | Oleamide | 21.49 | C18H35NO | [M + H]+ | 282.27914 | 282.279 | −0.50 | 265.25250 | Database, literature [23,25] | + | + |
73 | Organic acids | Palmitoleic Acid | 21.65 | C16H30O2 | [M + H]+ | 255.23186 | 255.23196 | 0.40 | 237.22130 | Database | + | + |
74 | Steroid | Ergosterol * | 21.85 | C28H44O | [M + H]+ | 397.34649 | 397.34674 | 0.63 | 379.33572 | Database | + | − |
75 | Steroid | Ergosterol endoperoxide | 21.97 | C28H44O3 | [M + H]+ | 429.33632 | 429.33643 | 0.25 | 411.32599, 393.31552 | Database, literature [26] | + | − |
76 | Organic acids | Palmitic acid * | 22.58 | C16H32O2 | [M − H]− | 255.23295 | 255.23296 | 0.03 | 256.23624 | Database, literature [25] | + | + |
77 | Organic acids | Oleic acid | 22.89 | C18H34O2 | [M − H]− | 281.24860 | 281.24869 | 0.31 | 282.25192 | Database | + | + |
78 | Esters | Linolenic acid ethyl ester | 23.57 | C20H34O2 | [M + H]+ | 307.26316 | 307.26355 | 1.28 | 123.11696 | Database | + | − |
79 | Esters | Ethyl oleate | 26.10 | C20H38O2 | [M + H]+ | 311.29446 | 311.29456 | 0.33 | 265.25259 | Database | + | + |
ID | Similarity | ID | Similarity |
---|---|---|---|
S1 | 0.971 | S6 | 0.984 |
S2 | 0.992 | S7 | 0.975 |
S3 | 0.986 | S8 | 0.999 |
S4 | 0.978 | S9 | 0.999 |
S5 | 0.998 | S10 | 0.996 |
Compound | Regression Equation | R2 | Linear Range (µg/mL) |
---|---|---|---|
Uracil | Y = 9,189,836.9096 X − 8756.4263 | 0.9998 | 0.99–32.84 |
Guanosine | Y = 6,907,785.2972 X − 6209.2408 | 0.9998 | 1.03–34.34 |
Adenosine | Y = 6,569,226.0327 X − 10,826.0629 | 0.9996 | 1.77–58.82 |
5-HMF | Y = 5,677,358.0948 X − 12,014.4694 | 0.9996 | 2.22–74.12 |
Daidzin | Y = 9,104,442.8249 X − 2582.8260 | 0.9997 | 0.30–10.15 |
Genistin | Y = 18,562,283.4133 X − 5566.5236 | 0.9997 | 0.27–8.92 |
Glycitein | Y = 13,180,712.1644 X − 5008.3407 | 0.9997 | 0.40–13.24 |
Daidzein | Y = 18,397,395.8876 X − 17,212.7445 | 0.9998 | 1.08–36.03 |
Genistein | Y = 29,448,803.7592 X − 3572.4452 | 0.9997 | 0.76–25.29 |
Ergosterol | Y = 2,134,505.6341 X − 2630.5954 | 0.9999 | 1.91–63.73 |
Compound | Sampling Volume/g | Sample Content/mg | Addition/mg | Measured Amount/mg | Recovery Rate/% | Average Recovery Rate/% | RSD/% |
---|---|---|---|---|---|---|---|
Uracil | 0.5001 | 0.1754 | 0.1620 | 0.3335 | 97.59 | 99.34 | 1.48 |
0.5004 | 0.1755 | 0.1620 | 0.3382 | 100.43 | |||
0.5003 | 0.1755 | 0.1620 | 0.3389 | 100.86 | |||
0.5001 | 0.1754 | 0.1620 | 0.3381 | 100.43 | |||
0.5002 | 0.1755 | 0.1620 | 0.3336 | 97.59 | |||
0.5001 | 0.1754 | 0.1620 | 0.3360 | 99.14 | |||
Guanosine | 0.5001 | 0.1093 | 0.0997 | 0.2066 | 97.59 | 98.06 | 2.12 |
0.5004 | 0.1093 | 0.0997 | 0.2071 | 98.09 | |||
0.5003 | 0.1093 | 0.0997 | 0.2085 | 99.50 | |||
0.5001 | 0.1093 | 0.0997 | 0.2088 | 99.80 | |||
0.5002 | 0.1093 | 0.0997 | 0.2032 | 94.18 | |||
0.5001 | 0.1093 | 0.0997 | 0.2082 | 99.20 | |||
Adenosine | 0.5001 | 0.2323 | 0.2085 | 0.4367 | 98.03 | 98.77 | 1.81 |
0.5004 | 0.2325 | 0.2085 | 0.4421 | 100.53 | |||
0.5003 | 0.2324 | 0.2085 | 0.4386 | 98.90 | |||
0.5001 | 0.2323 | 0.2085 | 0.4321 | 95.83 | |||
0.5002 | 0.2324 | 0.2085 | 0.4423 | 100.67 | |||
0.5001 | 0.2323 | 0.2085 | 0.4380 | 98.66 | |||
5-HMF | 0.5001 | 0.2062 | 0.2230 | 0.4252 | 98.21 | 98.26 | 2.87 |
0.5004 | 0.2063 | 0.2230 | 0.4365 | 103.23 | |||
0.5003 | 0.2063 | 0.2230 | 0.4219 | 96.68 | |||
0.5001 | 0.2062 | 0.2230 | 0.4227 | 97.09 | |||
0.5002 | 0.2062 | 0.2230 | 0.4276 | 99.28 | |||
0.5001 | 0.2062 | 0.2230 | 0.4182 | 95.07 | |||
Daidzin | 0.5001 | 0.0033 | 0.0055 | 0.0086 | 96.36 | 98.18 | 2.34 |
0.5004 | 0.0033 | 0.0055 | 0.0088 | 100.00 | |||
0.5003 | 0.0033 | 0.0055 | 0.0087 | 98.18 | |||
0.5001 | 0.0033 | 0.0055 | 0.0089 | 101.82 | |||
0.5002 | 0.0033 | 0.0055 | 0.0086 | 96.36 | |||
0.5001 | 0.0033 | 0.0055 | 0.0086 | 96.36 | |||
Genistin | 0.5001 | 0.0022 | 0.0035 | 0.0056 | 97.14 | 96.67 | 1.21 |
0.5004 | 0.0022 | 0.0035 | 0.0055 | 94.29 | |||
0.5003 | 0.0022 | 0.0035 | 0.0056 | 97.14 | |||
0.5001 | 0.0022 | 0.0035 | 0.0056 | 97.14 | |||
0.5002 | 0.0022 | 0.0035 | 0.0056 | 97.14 | |||
0.5001 | 0.0022 | 0.0035 | 0.0056 | 97.14 | |||
Glycitein | 0.5001 | 0.0152 | 0.0311 | 0.0462 | 99.68 | 98.34 | 2.09 |
0.5004 | 0.0152 | 0.0311 | 0.0465 | 100.64 | |||
0.5003 | 0.0152 | 0.0311 | 0.0453 | 96.78 | |||
0.5001 | 0.0152 | 0.0311 | 0.0449 | 95.50 | |||
0.5002 | 0.0152 | 0.0311 | 0.0455 | 97.43 | |||
0.5001 | 0.0152 | 0.0311 | 0.0463 | 100.00 | |||
Daidzein | 0.5001 | 0.0617 | 0.0757 | 0.1364 | 98.68 | 99.10 | 1.79 |
0.5004 | 0.0617 | 0.0757 | 0.1368 | 99.21 | |||
0.5003 | 0.0617 | 0.0757 | 0.1342 | 95.77 | |||
0.5001 | 0.0617 | 0.0757 | 0.1377 | 100.40 | |||
0.5002 | 0.0617 | 0.0757 | 0.1376 | 100.26 | |||
0.5001 | 0.0617 | 0.0757 | 0.1376 | 100.26 | |||
Genistein | 0.5001 | 0.0683 | 0.0841 | 0.1497 | 96.79 | 99.07 | 1.66 |
0.5004 | 0.0683 | 0.0841 | 0.1532 | 100.95 | |||
0.5003 | 0.0683 | 0.0841 | 0.1524 | 100.00 | |||
0.5001 | 0.0683 | 0.0841 | 0.1528 | 100.48 | |||
0.5002 | 0.0683 | 0.0841 | 0.1507 | 97.98 | |||
0.5001 | 0.0683 | 0.0841 | 0.1509 | 98.22 | |||
Ergosterol | 0.5001 | 0.2453 | 0.2313 | 0.4677 | 96.15 | 99.65 | 2.60 |
0.5004 | 0.2455 | 0.2313 | 0.4748 | 99.14 | |||
0.5003 | 0.2454 | 0.2313 | 0.4857 | 103.89 | |||
0.5001 | 0.2453 | 0.2313 | 0.4759 | 99.70 | |||
0.5002 | 0.2454 | 0.2313 | 0.4727 | 98.27 | |||
0.5001 | 0.2453 | 0.2313 | 0.4784 | 100.78 |
Sample | Uracil | Guanosine | Adenosine | 5-HMF | Daidzin | Genistin | Glycitein | Daidzein | Genistein | Ergosterol |
---|---|---|---|---|---|---|---|---|---|---|
S1 | 0.3304 | 0.2040 | 0.4271 | 0.4582 | 0.0085 | 0.0061 | 0.0434 | 0.1560 | 0.1697 | 0.4910 |
S2 | 0.3018 | 0.1987 | 0.4512 | 0.4015 | 0.0087 | 0.0061 | 0.0462 | 0.1731 | 0.1838 | 0.4917 |
S3 | 0.2935 | 0.2095 | 0.4741 | 0.4140 | 0.0092 | 0.0063 | 0.0466 | 0.1773 | 0.1859 | 0.4841 |
S4 | 0.3369 | 0.1445 | 0.3113 | 0.4427 | 0.0069 | 0.0053 | 0.0336 | 0.1125 | 0.1262 | 0.4504 |
S5 | 0.3417 | 0.1806 | 0.3569 | 0.4508 | 0.0075 | 0.0057 | 0.0369 | 0.1285 | 0.1397 | 0.4523 |
S6 | 0.3151 | 0.1948 | 0.4415 | 0.4418 | 0.0086 | 0.0063 | 0.0447 | 0.1631 | 0.1728 | 0.4956 |
S7 | 0.3508 | 0.2185 | 0.4646 | 0.4123 | 0.0066 | 0.0043 | 0.0304 | 0.1234 | 0.1365 | 0.4906 |
S8 | 0.2187 | 0.2547 | 0.6121 | 0.3363 | 0.0112 | 0.0088 | 0.0613 | 0.2439 | 0.2288 | 0.4980 |
S9 | 0.2400 | 0.2158 | 0.5083 | 0.3465 | 0.0096 | 0.0071 | 0.0526 | 0.2023 | 0.1971 | 0.4666 |
S10 | 0.1814 | 0.2617 | 0.6307 | 0.2788 | 0.0117 | 0.0080 | 0.0625 | 0.2570 | 0.2375 | 0.4878 |
S11 | 0.0327 | 0.0244 | 0.0308 | / | 0.0524 | 0.0394 | 0.0156 | 0.0237 | 0.0190 | / |
Control | Batch Number | Manufacturing Company |
---|---|---|
Cytidine | B20073 | Shanghai Yuanye Biotechnology Co., Shanghai, China |
Betaine | PS012048 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Nicotinic acid | PS020097 | Chengdu Pusi Biotechnology Co., Chengdu, China |
2,6-Dihydroxypurine | PS020191 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Uridine | 887-200202 | China National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China |
Adenosine | 110879-200202 | China National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China |
Guanosine | PS010291 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Uracil | U13135C30 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Ascorbic acid | J04A10R84808 | Shanghai Yuanye Biotechnology Co., Shanghai, China |
5-Methyl-2-furaldehyde | J23S6X3622 | Shanghai Yuanye Biotechnology Co., Shanghai, China |
5-hydroxymethylfurfural | H81835D5F | Shanghai Jiji Biochemical Technology Co., Shanghai, China |
D-phenylalanine | H06A8H33287 | Shanghai Yuanye Biotechnology Co., Shanghai, China |
Levetiracetam | PS230518-15 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Benzoic acid | PS161011-06 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Daidzin | PS011899 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Phenacetin | PS230518-16 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Genistin | 111709-200501 | China National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China |
Daidzein | B20227 | Shanghai Yuanye Biotechnology Co., Shanghai, China |
Glycitein | PS011931 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Genistein | 111704-200501 | China National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China |
Naringenin | PS010355 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Kaempferide | PS011599 | Chengdu Pusi Biotechnology Co., Chengdu, China |
Ergosterol | 111845-202105 | China National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China |
Palmitic acid | PS001166 | Chengdu Pusi Biotechnology Co., Chengdu, China |
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Wu, Y.; Zhang, H.; Zhu, J.; Zhang, Z.; Ma, S.; Zhao, Y.; Wang, Y.; Yuan, J.; Guo, X.; Li, Y.; et al. The Effect of Fermentation on the Chemical Constituents of Gastrodia Tuber Hallimasch Powder (GTHP) Estimated by UHPLC-Q-Orbitrap HRMS and HPLC. Molecules 2024, 29, 1663. https://doi.org/10.3390/molecules29071663
Wu Y, Zhang H, Zhu J, Zhang Z, Ma S, Zhao Y, Wang Y, Yuan J, Guo X, Li Y, et al. The Effect of Fermentation on the Chemical Constituents of Gastrodia Tuber Hallimasch Powder (GTHP) Estimated by UHPLC-Q-Orbitrap HRMS and HPLC. Molecules. 2024; 29(7):1663. https://doi.org/10.3390/molecules29071663
Chicago/Turabian StyleWu, Yaning, Hongwei Zhang, Jianguang Zhu, Zhenling Zhang, Songbo Ma, Yongqi Zhao, Yiming Wang, Jun Yuan, Xing Guo, Yajing Li, and et al. 2024. "The Effect of Fermentation on the Chemical Constituents of Gastrodia Tuber Hallimasch Powder (GTHP) Estimated by UHPLC-Q-Orbitrap HRMS and HPLC" Molecules 29, no. 7: 1663. https://doi.org/10.3390/molecules29071663