Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro
Abstract
:1. Introduction
2. Material and Methods
2.1. Chemicals and Animals
2.2. SCE Assay
2.3. DPPH Radical Scavenging Activity
2.4. Ferric Thiocyanate Assay
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusion
References
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Compound | RTa | CAS no. | Area (%) |
---|---|---|---|
(E)-β-Farnecene | 38.46 | 28973-97-9 | 28.17 |
Germacrene-d | 39.23 | 23986-74-5 | 2.19 |
Unidentified sesquiterpene | 40.07 | 1.40 | |
Unidentified sesquiterpene | 41.17 | 0.78 | |
(Z,E)-α-Farnecene | 41.35 | 26560-14-5 | 1.59 |
Unidentified sesquiterpene | 48.52 | 0.71 | |
α-Bisabolol oxide A | 54.46 | 22567-36-8 | 41.77 |
α-Bisabolol oxide B | 49.28 | 26184-88-3 | 4.31 |
α-Bisabolol oxide | 50.65 | 22567-38-0 | 5.30 |
α-Bisabolol | 51.18 | 515-69-5 | 2.31 |
Chamazulene | 52.80 | 529-05-5 | 2.39 |
1,6-Dioxaspiro[4,4]non-3-ene,2-(2,4hexadyn-1-ylidene) | 60.73 | 2.19 | |
Hexatriacontane | 67.49 | 630-06-8 | 0.50 |
Agent | Dose (mg/kg) | SCE ± S.D. | Inhibition (%) |
---|---|---|---|
Controla | 0 | * 1.90 ± 0.40 | |
CEO | 500 | * 2.36 ± 0.44 | |
DAU | 10 | 11.21 ± 1.38 | |
CEO+DAU | 5+10 | * 6.78 ± 1.05 | 47.5 |
CEO+DAU | 50+10 | * 5.44 ± 0.33 | 61.9 |
CEO+DAU | 500+10 | * 2.50 ± 0.31 | 93.5 |
Agent | Concentration(mg/mL) | Absorbance OD (517 nm) | Absorbance Inhibition (%) |
---|---|---|---|
DPPH | 0.04 | 1.059 ± 0.001 | |
α-cariophylene | 100 | 1.048 ± 0.009 | 1.0 |
α-tocopherol | 1 | 0.362 ± 0.015* | 65.8 |
CEO | 5 | 1.057 ± 0.003 | 0.001 |
CEO | 10 | 1.013 ± 0.004* | 4.3 |
CEO | 50 | 0.872 ± 0.014* | 17.6 |
CEO | 100 | 0.621 ± 0.022* | 41.3 |
CEO | 500 | 0.208 ± 0.013* | 80.4 |
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Hernández-Ceruelos, A.; Madrigal-Santillán, E.; Morales-González, J.A.; Chamorro-Cevallos, G.; Cassani-Galindo, M.; Madrigal-Bujaidar, E. Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro. Int. J. Mol. Sci. 2010, 11, 3793-3802. https://doi.org/10.3390/ijms11103793
Hernández-Ceruelos A, Madrigal-Santillán E, Morales-González JA, Chamorro-Cevallos G, Cassani-Galindo M, Madrigal-Bujaidar E. Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro. International Journal of Molecular Sciences. 2010; 11(10):3793-3802. https://doi.org/10.3390/ijms11103793
Chicago/Turabian StyleHernández-Ceruelos, Alejandra, Eduardo Madrigal-Santillán, José Antonio Morales-González, Germán Chamorro-Cevallos, Martha Cassani-Galindo, and Eduardo Madrigal-Bujaidar. 2010. "Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro" International Journal of Molecular Sciences 11, no. 10: 3793-3802. https://doi.org/10.3390/ijms11103793